DHTKD1 (NM_018706) Human Tagged ORF Clone

CAT#: RC200967

DHTKD1 (Myc-DDK-tagged)-Human dehydrogenase E1 and transketolase domain containing 1 (DHTKD1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC200967

• 

  Western blot validation of overexpression lysate (Cat# LY412899) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200967 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DHTKD1
• Synonyms: AAKAD; AMOXAD; CMT2Q
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200967 representing NM_018706
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCTGCTACTGCGGCAGCAGCACGACGGGGCCTCGGCCGGGCTCTCCCTCTCCTCTGGCGTGGCT
  ACCAGACCGAGCGGGGCGTTTACGGCTACCGGCCGAGGAAGCCCGAGAGCCGCGAGCCCCAGGGCGCCCT
  GGAGCGCCCCCCAGTTGATCATGGCCTTGCCAGGTTGGTGACAGTATATTGTGAGCATGGTCATAAAGCT
  GCCAAAATCAACCCCCTCTTCACCGGACAAGCCCTGCTGGAGAATGTGCCTGAAATCCAAGCCCTGGTGC
  AGACACTGCAGGGACCCTTCCACACGGCAGGATTATTGAACATGGGGAAGGAAGAGGCCTCACTTGAGGA
  AGTGTTAGTCTATCTCAATCAAATCTACTGTGGGCAGATTTCTATTGAAACCTCCCAACTTCAGAGCCAG
  GATGAGAAAGACTGGTTTGCCAAGCGGTTTGAGGAACTGCAAAAGGAGACGTTTACCACAGAAGAGCGAA
  AACATCTGTCGAAACTAATGCTGGAATCTCAGGAGTTTGACCACTTTCTGGCCACCAAGTTCTCGACAGT
  GAAGCGATATGGAGGCGAAGGGGCTGAAAGCATGATGGGCTTTTTCCACGAGCTGCTGAAAATGTCGGCC
  TACAGCGGGATCACTGATGTCATTATTGGGATGCCCCATAGAGGGAGGCTGAATTTATTGACAGGCCTTC
  TGCAGTTCCCTCCAGAGCTGATGTTCCGTAAAATGCGAGGCTTAAGTGAATTTCCAGAGAATTTCTCAGC
  CACTGGAGACGTCCTGTCTCACCTGACCTCCTCTGTGGACCTGGACTTTGGGGCGCACCATCCCCTCCAT
  GTGACAATGTTGCCCAATCCCTCGCACCTGGAGGCCGTCAACCCCGTGGCCGTGGGCAAAACTCGCGGCA
  GGCAGCAGTCTCTCCAAGACGGCGATTACTCTCCAGACAACTCAGCCCAGCCGGGGGACAGGGTCATTTG
  CTTACAGGTCCATGGTGATGCTTCTTTCTGTGGTCAAGGGATTGTTCCTGAAACATTCACGCTGTCCAAT
  CTCCCACATTTCAGAATTGGTGGGAGTGTGCATTTGATTGTTAATAACCAGCTGGGTTACACCACTCCAG
  CTGAAAGAGGAAGGTCTTCTTTATACTGCAGTGATATTGGGAAGCTTGTGGGCTGTGCCATCATCCATGT
  CAATGGAGACAGCCCAGAGGAAGTGGTCCGTGCCACACGACTGGCTTTTGAATACCAACGCCAGTTCCGC
  AAGGATGTGATTATTGATCTGTTGTGCTACAGGCAGTGGGGCCACAATGAGCTGGATGAGCCATTCTACA
  CCAACCCCATCATGTACAAAATCATCAGAGCTCGAAAGAGCATTCCAGACACATATGCAGAGCACCTCAT
  TGCTGGCGGACTCATGACGCAGGAGGAGGTGTCTGAAATAAAATCCTCCTACTATGCCAAGTTGAATGAT
  CACTTAAATAACATGGCCCACTACAGGCCCCCTGCCCTGAACCTGCAGGCCCACTGGCAGGGCCTGGCTC
  AGCCAGAAGCGCAAATCACCACCTGGAGTACAGGTGTGCCCCTCGACCTCCTGCGGTTTGTTGGCATGAA
  GTCTGTAGAGGTGCCAAGAGAGCTGCAGATGCACAGTCACCTGCTGAAGACACATGTTCAGTCCAGAATG
  GAGAAGATGATGGACGGAATCAAGCTAGACTGGGCCACCGCGGAAGCTCTTGCCTTGGGTTCTTTACTTG
  CTCAAGGTTTTAATGTTCGTCTAAGTGGCCAAGATGTTGGTCGTGGAACTTTCAGTCAGAGGCATGCAAT
  GGTGGTTTGCCAGGAGACGGATGACACCTACATCCCCCTGAACCATATGGACCCAAATCAGAAGGGGTTT
  CTAGAGGTCAGCAACAGCCCCCTGTCAGAAGAGGCCGTCCTGGGATTCGAATATGGGATGAGCATTGAGA
  GCCCAAAGTTACTGCCCCTGTGGGAGGCACAGTTTGGCGATTTCTTCAATGGTGCCCAGATCATCTTTGA
  CACATTCATCTCTGGAGGAGAGGCCAAGTGGCTCCTACAAAGCGGCATTGTCATCCTCCTTCCACATGGC
  TACGATGGGGCTGGGCCAGACCACTCATCCTGTCGAATAGAGCGTTTCCTGCAGATGTGTGACAGTGCGG
  AAGAGGGGGTGGACGGAGACACTGTGAACATGTTTGTGGTTCACCCAACAACTCCTGCACAGTATTTCCA
  CTTGCTTAGGAGACAGATGGTCCGGAACTTCAGAAAACCACTCATTGTTGCTTCCCCTAAGATGTTACTC
  AGGCTCCCGGCAGCCGTGTCAACTCTTCAAGAAATGGCACCAGGAACAACATTTAACCCGGTCATTGGTG
  ATTCATCTGTGGATCCAAAAAAGGTTAAGACCCTCGTGTTCTGCTCCGGCAAACATTTCTACTCCCTGGT
  GAAACAAAGAGAATCTCTGGGGGCCAAGAAGCATGACTTTGCCATCATCCGAGTAGAGGAACTCTGCCCC
  TTCCCGTTGGATTCTTTACAGCAAGAGATGAGCAAATACAAACATGTTAAAGATCATATTTGGAGTCAGG
  AGGAACCTCAGAACATGGGTCCGTGGTCGTTTGTTTCTCCAAGGTTTGAAAAGCAGCTGGCCTGCAAGCT
  CCGTCTGGTGGGCCGGCCCCCTTTGCCAGTACCCGCTGTAGGAATTGGCACAGTTCACTTGCACCAGCAT
  GAAGATATCCTCGCCAAGACCTTCGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200967 representing NM_018706
  Red=Cloning site Green=Tags(s)
  
  MASATAAAARRGLGRALPLLWRGYQTERGVYGYRPRKPESREPQGALERPPVDHGLARLVTVYCEHGHKA
  AKINPLFTGQALLENVPEIQALVQTLQGPFHTAGLLNMGKEEASLEEVLVYLNQIYCGQISIETSQLQSQ
  DEKDWFAKRFEELQKETFTTEERKHLSKLMLESQEFDHFLATKFSTVKRYGGEGAESMMGFFHELLKMSA
  YSGITDVIIGMPHRGRLNLLTGLLQFPPELMFRKMRGLSEFPENFSATGDVLSHLTSSVDLDFGAHHPLH
  VTMLPNPSHLEAVNPVAVGKTRGRQQSLQDGDYSPDNSAQPGDRVICLQVHGDASFCGQGIVPETFTLSN
  LPHFRIGGSVHLIVNNQLGYTTPAERGRSSLYCSDIGKLVGCAIIHVNGDSPEEVVRATRLAFEYQRQFR
  KDVIIDLLCYRQWGHNELDEPFYTNPIMYKIIRARKSIPDTYAEHLIAGGLMTQEEVSEIKSSYYAKLND
  HLNNMAHYRPPALNLQAHWQGLAQPEAQITTWSTGVPLDLLRFVGMKSVEVPRELQMHSHLLKTHVQSRM
  EKMMDGIKLDWATAEALALGSLLAQGFNVRLSGQDVGRGTFSQRHAMVVCQETDDTYIPLNHMDPNQKGF
  LEVSNSPLSEEAVLGFEYGMSIESPKLLPLWEAQFGDFFNGAQIIFDTFISGGEAKWLLQSGIVILLPHG
  YDGAGPDHSSCRIERFLQMCDSAEEGVDGDTVNMFVVHPTTPAQYFHLLRRQMVRNFRKPLIVASPKMLL
  RLPAAVSTLQEMAPGTTFNPVIGDSSVDPKKVKTLVFCSGKHFYSLVKQRESLGAKKHDFAIIRVEELCP
  FPLDSLQQEMSKYKHVKDHIWSQEEPQNMGPWSFVSPRFEKQLACKLRLVGRPPLPVPAVGIGTVHLHQH
  EDILAKTFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018706
• ORF Size: 2757 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018706.1
• RefSeq Size: 5162 bp
• RefSeq ORF: 2760 bp
• Locus ID: 55526
• UniProt ID: Q96HY7
• Cytogenetics: 10p14
• Domains: E1_dehydrog, transket_pyr
• MW: 102.9 kDa
• Gene Summary: This gene encodes a component of a mitochondrial 2-oxoglutarate-dehydrogenase-complex-like protein involved in the degradation pathways of several amino acids, including lysine. Mutations in this gene are associated with 2-aminoadipic 2-oxoadipic aciduria and Charcot-Marie-Tooth Disease Type 2Q. [provided by RefSeq, May 2013]