RNPEP (NM_020216) Human Tagged ORF Clone

CAT#: RC200756

RNPEP (Myc-DDK-tagged)-Human arginyl aminopeptidase (aminopeptidase B) (RNPEP)

Product Images

Circular map for RC200756

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RNPEP (Cat# RC200756, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNPEP(Cat# TA503948). Positive lysates LY412581 (100ug) and LC412581 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY412581) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200756 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RNPEP protein (Cat# TP300756). The protein was produced from HEK293T cells transfected with RNPEP cDNA clone (Cat# RC200756) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNPEP
• Synonyms: AP-B; APB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200756 representing NM_020216
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCGGCGAGCATTCCCCCGGCAGCGGCGCGGCCCGGCGGCCGCTGCACTCCGCGCAGGCTGTGG
  ACGTGGCCTCGGCCTCCAACTTCCGGGCCTTTGAGCTGCTGCACTTGCACCTGGACCTGCGGGCTGAGTT
  CGGGCCTCCAGGGCCCGGCGCAGGGAGCCGGGGGCTGAGCGGCACCGCGGTCCTGGACCTGCGCTGCCTG
  GAGCCCGAGGGCGCCGCCGAGCTGCGGCTGGACTCGCACCCGTGCCTGGAGGTGACGGCGGCGGCGCTGC
  GGCGGGAGCGGCCCGGCTCGGAGGAGCCGCCTGCGGAGCCCGTGAGCTTCTACACGCAGCCCTTCTCGCA
  CTATGGCCAGGCCCTGTGCGTGTCCTTCCCGCAGCCCTGCCGCGCCGCCGAGCGCCTCCAGGTGCTGCTC
  ACCTACCGCGTCGGGGAGGGACCCGGGGTTTGCTGGTTGGCTCCCGAGCAGACAGCAGGAAAGAAGAAGC
  CCTTCGTGTACACCCAGGGCCAGGCTGTCCTAAACCGGGCCTTCTTCCCTTGCTTCGACACGCCTGCTGT
  TAAATACAAGTATTCAGCTCTTATTGAGGTCCCAGATGGCTTCACAGCTGTGATGAGTGCTAGCACCTGG
  GAGAAGAGAGGTCCAAATAAGTTCTTCTTCCAGATGTGTCAGCCCATCCCCTCCTATCTGATAGCTTTGG
  CCATCGGAGATCTGGTTTCGGCTGAAGTTGGACCCAGGAGCCGGGTGTGGGCTGAGCCCTGCCTGATTGA
  TGCTGCCAAGGAGGAGTACAACGGGGTGATAGAAGAATTTTTGGCAACAGGAGAGAAGCTTTTTGGACCT
  TATGTTTGGGGAAGGTATGACTTGCTCTTCATGCCACCGTCCTTTCCATTTGGAGGAATGGAGAACCCTT
  GTCTGACCTTTGTCACCCCCTGCCTGCTAGCTGGGGACCGCTCCTTGGCAGATGTCATCATCCATGAGAT
  CTCCCACAGTTGGTTTGGGAACCTGGTCACCAACGCCAACTGGGGTGAATTCTGGCTCAATGAAGGTTTC
  ACCATGTACGCCCAGAGGAGGATCTCCACCATCCTCTTTGGCGCTGCGTACACCTGCTTGGAGGCTGCAA
  CGGGGCGGGCTCTGCTGCGTCAACACATGGACATCACTGGAGAGGAAAACCCACTCAACAAGCTCCGCGT
  GAAGATTGAACCAGGCGTTGACCCGGACGACACCTATAATGAGACCCCCTACGAGAAAGGTTTCTGCTTT
  GTCTCATACCTGGCCCACTTGGTGGGTGATCAGGATCAGTTTGACAGTTTTCTCAAGGCCTATGTGCATG
  AATTCAAATTCCGAAGCATCTTAGCCGATGACTTTCTGGACTTCTACTTGGAATATTTCCCTGAGCTTAA
  GAAAAAGAGAGTGGATATCATTCCAGGTTTTGAGTTTGATCGATGGCTGAATACCCCCGGCTGGCCCCCG
  TACCTCCCTGATCTCTCCCCTGGGGACTCACTCATGAAGCCTGCTGAAGAGCTAGCCCAACTGTGGGCAG
  CCGAGGAGCTGGACATGAAGGCCATTGAAGCCGTGGCCATCTCTCCCTGGAAGACCTACCAGCTGGTCTA
  CTTCCTGGATAAGATCCTCCAGAAATCCCCTCTCCCTCCTGGGAATGTGAAAAAACTTGGAGACACATAC
  CCAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCAAATCGTCCTTAAGAACGACC
  ACCAGGAAGATTTCTGGAAAGTGAAGGAGTTCCTGCATAACCAGGGGAAGCAGAAGTATACACTTCCGCT
  GTACCACGCAATGATGGGTGGCAGTGAGGTGGCCCAGACCCTCGCCAAGGAGACTTTTGCATCCACCGCC
  TCCCAGCTCCACAGCAATGTTGTCAACTATGTCCAGCAGATCGTGGCACCCAAGGGCAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200756 representing NM_020216
  Red=Cloning site Green=Tags(s)
  
  MASGEHSPGSGAARRPLHSAQAVDVASASNFRAFELLHLHLDLRAEFGPPGPGAGSRGLSGTAVLDLRCL
  EPEGAAELRLDSHPCLEVTAAALRRERPGSEEPPAEPVSFYTQPFSHYGQALCVSFPQPCRAAERLQVLL
  TYRVGEGPGVCWLAPEQTAGKKKPFVYTQGQAVLNRAFFPCFDTPAVKYKYSALIEVPDGFTAVMSASTW
  EKRGPNKFFFQMCQPIPSYLIALAIGDLVSAEVGPRSRVWAEPCLIDAAKEEYNGVIEEFLATGEKLFGP
  YVWGRYDLLFMPPSFPFGGMENPCLTFVTPCLLAGDRSLADVIIHEISHSWFGNLVTNANWGEFWLNEGF
  TMYAQRRISTILFGAAYTCLEAATGRALLRQHMDITGEENPLNKLRVKIEPGVDPDDTYNETPYEKGFCF
  VSYLAHLVGDQDQFDSFLKAYVHEFKFRSILADDFLDFYLEYFPELKKKRVDIIPGFEFDRWLNTPGWPP
  YLPDLSPGDSLMKPAEELAQLWAAEELDMKAIEAVAISPWKTYQLVYFLDKILQKSPLPPGNVKKLGDTY
  PSISNARNAELRLRWGQIVLKNDHQEDFWKVKEFLHNQGKQKYTLPLYHAMMGGSEVAQTLAKETFASTA
  SQLHSNVVNYVQQIVAPKGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020216
• ORF Size: 1950 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020216.3, NP_064601.3
• RefSeq Size: 2430 bp
• RefSeq ORF: 1953 bp
• Locus ID: 6051
• UniProt ID: Q9H4A4
• Cytogenetics: 1q32.1
• Domains: Peptidase_M1
• Protein Families: Druggable Genome, Protease, Secreted Protein
• MW: 72.4 kDa
• Gene Summary: Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4) (By similarity).[UniProtKB/Swiss-Prot Function]