NAGA (NM_000262) Human Tagged ORF Clone

CAT#: RC200707

NAGA (Myc-DDK-tagged)-Human N-acetylgalactosaminidase, alpha- (NAGA)

Product Images

Circular map for RC200707

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NAGA (Cat# RC200707, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NAGA (Cat# TA811320)(1:500). Positive lysates LY400100 (100ug) and LC400100 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400100) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200707 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NAGA protein (Cat# TP300707). The protein was produced from HEK293T cells transfected with NAGA cDNA clone (Cat# RC200707) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NAGA
• Synonyms: D22S674; GALB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200707 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGAAGACAGTGCTCTTGCTGGGACATGTGGCCCAGGTGCTGATGCTGGACAATGGGCTCCTGC
  AGACACCACCCATGGGCTGGCTGGCCTGGGAACGCTTCCGCTGCAACATTAACTGTGATGAGGACCCAAA
  GAACTGCATAAGTGAACAGCTCTTCATGGAGATGGCTGACCGGATGGCACAGGATGGATGGCGGGACATG
  GGCTACACATACCTCAACATTGATGACTGCTGGATCGGTGGTCGCGATGCCAGTGGCCGCCTGATGCCAG
  ATCCCAAGCGCTTCCCTCATGGCATTCCTTTCCTGGCTGACTACGTTCACTCCCTGGGCCTGAAGTTGGG
  TATCTACGCGGACATGGGCAACTTCACCTGCATGGGTTACCCAGGCACCACACTGGACAAGGTGGTCCAG
  GATGCTCAGACCTTCGCCGAGTGGAAGGTAGACATGCTCAAGCTGGATGGCTGCTTCTCCACCCCCGAGG
  AGCGGGCCCAGGGGTACCCCAAGATGGCTGCTGCCCTGAATGCCACAGGCCGCCCCATCGCCTTCTCCTG
  CAGCTGGCCAGCCTATGAAGGCGGCCTCCCCCCAAGGGTGAACTACAGTCTGCTGGCGGACATCTGCAAC
  CTCTGGCGTAACTATGATGACATCCAGGACTCCTGGTGGAGCGTGCTCTCCATCCTGAATTGGTTCGTGG
  AGCACCAGGACATACTGCAGCCAGTGGCCGGCCCTGGGCACTGGAATGACCCTGACATGCTGCTCATTGG
  GAACTTTGGTCTCAGCTTAGAGCAATCCCGGGCCCAGATGGCCCTGTGGACGGTGCTGGCAGCCCCCCTC
  TTGATGTCCACAGACCTGCGTACCATCTCCGCCCAGAACATGGACATTCTGCAGAATCCACTCATGATCA
  AAATCAACCAGGATCCCTTAGGCATCCAGGGACGCAGGATTCACAAGGAAAAATCTCTCATCGAAGTGTA
  CATGCGGCCTCTGTCCAACAAGGCTAGCGCCTTAGTCTTCTTCAGCTGCAGGACCGATATGCCTTATCGC
  TACCACTCCTCCCTTGGCCAGCTGAACTTCACCGGGTCTGTGATATATGAGGCCCAGGACGTCTACTCAG
  GTGACATCATCAGTGGCCTCCGAGATGAAACCAACTTCACAGTGATCATCAACCCTTCAGGGGTAGTGAT
  GTGGTACCTGTATCCCATCAAGAACCTGGAGATGTCCCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200707 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLLKTVLLLGHVAQVLMLDNGLLQTPPMGWLAWERFRCNINCDEDPKNCISEQLFMEMADRMAQDGWRDM
  GYTYLNIDDCWIGGRDASGRLMPDPKRFPHGIPFLADYVHSLGLKLGIYADMGNFTCMGYPGTTLDKVVQ
  DAQTFAEWKVDMLKLDGCFSTPEERAQGYPKMAAALNATGRPIAFSCSWPAYEGGLPPRVNYSLLADICN
  LWRNYDDIQDSWWSVLSILNWFVEHQDILQPVAGPGHWNDPDMLLIGNFGLSLEQSRAQMALWTVLAAPL
  LMSTDLRTISAQNMDILQNPLMIKINQDPLGIQGRRIHKEKSLIEVYMRPLSNKASALVFFSCRTDMPYR
  YHSSLGQLNFTGSVIYEAQDVYSGDIISGLRDETNFTVIINPSGVVMWYLYPIKNLEMSQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000262
• ORF Size: 1233 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000262.3
• RefSeq Size: 3726 bp
• RefSeq ORF: 1236 bp
• Locus ID: 4668
• UniProt ID: P17050
• Cytogenetics: 22q13.2
• Domains: Melibiase
• Protein Families: Druggable Genome
• Protein Pathways: Glycosphingolipid biosynthesis - globo series, Lysosome
• MW: 46.6 kDa
• Gene Summary: NAGA encodes the lysosomal enzyme alpha-N-acetylgalactosaminidase, which cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates. Mutations in NAGA have been identified as the cause of Schindler disease types I and II (type II also known as Kanzaki disease). [provided by RefSeq, Jul 2008]