HNRPUL1 (HNRNPUL1) (NM_007040) Human Tagged ORF Clone

CAT#: RC200576

HNRNPUL1 (Myc-DDK-tagged)-Human heterogeneous nuclear ribonucleoprotein U-like 1 (HNRNPUL1), transcript variant 1

Product Images

Circular map for RC200576

• 

  Western blot validation of overexpression lysate (Cat# LY416239) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200576 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HNRPUL1
• Synonyms: E1B-AP5; E1BAP5; HNRPUL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200576 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGCGCCGTCTGAAGGTGAACGAACTTCGCGAGGAGCTGCAGCGCCGCGGCCTGGACACTCGAG
  GCCTCAAGGCCGAGCTTGCTGAGCGGCTGCAGGCGGCGTTGGAGGCCGAGGAGCCTGACGACGAGCGGGA
  GCTCGACGCCGACGACGAACCGGGGCGACCCGGGCACATCAACGAGGAGGTCGAGACCGAGGGGGGCTCC
  GAGCTGGAGGGGACCGCGCAGCCACCGCCGCCCGGGCTGCAGCCGCACGCGGAGCCCGGCTGCTACTCGG
  GGCCGGACGGACATTATGCCATGGACAATATTACCAGGCAGAACCAATTCTACGATACCCAAGTCATCAA
  ACAAGAAAACGAGTCAGGCTACGAGAGGAGACCACTGGAAATGGAGCAGCAGCAGGCCTATCGTCCAGAA
  ATGAAGACAGAGATGAAGCAAGGAGCACCCACCAGCTTCCTCCCGCCTGAAGCTTCTCAACTCAAGCCAG
  ACAGGCAGCAATTCCAGAGTCGAAAGAGGCCTTATGAAGAAAACCGGGGACGGGGGTACTTTGAGCACCG
  AGAGGATAGGAGGGGCCGCTCTCCTCAGCCTCCTGCTGAAGAGGATGAAGATGACTTTGATGATACCCTT
  GTTGCTATTGACACCTATAACTGCGACCTCCACTTCAAGGTGGCCCGAGATCGGAGTAGTGGCTATCCGC
  TCACAATTGAGGGCTTTGCATACCTGTGGTCAGGAGCCCGTGCCAGCTATGGGGTCAGAAGGGGCCGTGT
  ATGCTTCGAGATGAAGATCAATGAGGAAATCTCCGTGAAGCACCTTCCGTCTACAGAGCCTGACCCCCAC
  GTGGTCCGTATCGGCTGGTCCCTGGACTCCTGCAGCACCCAGCTAGGCGAAGAGCCTTTCTCCTATGGCT
  ATGGAGGCACTGGGAAGAAGTCCACCAATAGCCGGTTTGAAAACTACGGAGACAAGTTTGCAGAGAACGA
  TGTGATTGGCTGCTTTGCGGATTTTGAATGTGGAAATGACGTGGAACTGTCTTTTACCAAGAATGGAAAG
  TGGATGGGCATTGCTTTCCGAATCCAGAAGGAAGCCTTGGGGGGTCAGGCCCTCTATCCTCATGTCCTGG
  TGAAGAATTGCGCAGTGGAGTTCAACTTCGGACAGAGAGCAGAGCCCTACTGTTCTGTCCTCCCGGGGTT
  TACCTTCATCCAGCACCTTCCCCTTAGTGAGCGTATCCGGGGCACCGTTGGACCAAAGAGCAAGGCAGAA
  TGTGAGATTCTGATGATGGTGGGCCTGCCTGCTGCTGGCAAGACCACATGGGCCATCAAACATGCAGCCT
  CCAACCCTTCCAAGAAGTACAACATCCTGGGTACCAATGCCATCATGGATAAGATGCGGGTGATGGGCCT
  ACGCCGGCAGCGGAACTATGCTGGCCGCTGGGATGTCCTGATCCAGCAGGCCACCCAGTGCCTCAACCGC
  CTCATCCAGATTGCTGCCCGCAAGAAACGCAACTATATCCTAGATCAGACAAATGTTTATGGGTCAGCCC
  AGAGACGAAAAATGAGACCATTTGAAGGCTTCCAGCGCAAAGCTATTGTAATTTGTCCCACTGACGAGGA
  CCTAAAAGACCGAACAATAAAGCGAACCGACGAGGAAGGGAAGGATGTCCCAGATCATGCGGTCTTAGAA
  ATGAAAGCCAACTTCACGTTGCCAGATGTTGGGGACTTCCTGGATGAGGTTCTGTTCATTGAGCTGCAGC
  GGGAGGAAGCGGACAAGCTAGTGAGGCAGTACAACGAGGAAGGCCGCAAGGCTGGGCCACCCCCTGAAAA
  GCGCTTTGACAACCGAGGTGGTGGTGGCTTCCGGGGCCGCGGGGGTGGTGGTGGCTTCCAGCGCTATGAA
  AACCGAGGACCCCCTGGAGGCAACCGTGGCGGCTTCCAGAACCGAGGGGGAGGCAGCGGTGGAGGAGGCA
  ACTACCGAGGAGGTTTCAACCGCAGCGGAGGTGGTGGCTATAGCCAGAACCGCTGGGGTAACAACAACCG
  GGATAACAACAACTCCAACAACAGAGGCAGCTACAACCGGGCTCCCCAGCAACAGCCGCCACCACAGCAG
  CCTCCGCCACCACAGCCACCACCCCAGCAGCCACCGCCACCACCCAGCTACAGCCCTGCTCGGAACCCCC
  CAGGGGCCAGCACCTACAATAAGAACAGCAACATCCCTGGCTCAAGCGCCAATACCAGCACCCCCACCGT
  CAGCAGCTACAGCCCTCCACAGCCGAGTTACAGCCAGCCACCCTACAACCAGGGAGGTTACAGCCAGGGC
  TACACAGCCCCACCGCCTCCACCTCCACCACCACCTGCCTACAACTATGGGAGCTACGGCGGTTACAACC
  CGGCCCCCTATACCCCACCGCCACCCCCCACCGCACAGACCTACCCTCAGCCCAGCTATAACCAGTATCA
  GCAGTATGCCCAGCAGTGGAACCAGTACTATCAGAACCAGGGCCAGTGGCCGCCATACTACGGGAACTAC
  GACTACGGGAGCTACTCCGGGAACACACAGGGTGGCACAAGTACACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200576 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDVRRLKVNELREELQRRGLDTRGLKAELAERLQAALEAEEPDDERELDADDEPGRPGHINEEVETEGGS
  ELEGTAQPPPPGLQPHAEPGCYSGPDGHYAMDNITRQNQFYDTQVIKQENESGYERRPLEMEQQQAYRPE
  MKTEMKQGAPTSFLPPEASQLKPDRQQFQSRKRPYEENRGRGYFEHREDRRGRSPQPPAEEDEDDFDDTL
  VAIDTYNCDLHFKVARDRSSGYPLTIEGFAYLWSGARASYGVRRGRVCFEMKINEEISVKHLPSTEPDPH
  VVRIGWSLDSCSTQLGEEPFSYGYGGTGKKSTNSRFENYGDKFAENDVIGCFADFECGNDVELSFTKNGK
  WMGIAFRIQKEALGGQALYPHVLVKNCAVEFNFGQRAEPYCSVLPGFTFIQHLPLSERIRGTVGPKSKAE
  CEILMMVGLPAAGKTTWAIKHAASNPSKKYNILGTNAIMDKMRVMGLRRQRNYAGRWDVLIQQATQCLNR
  LIQIAARKKRNYILDQTNVYGSAQRRKMRPFEGFQRKAIVICPTDEDLKDRTIKRTDEEGKDVPDHAVLE
  MKANFTLPDVGDFLDEVLFIELQREEADKLVRQYNEEGRKAGPPPEKRFDNRGGGGFRGRGGGGGFQRYE
  NRGPPGGNRGGFQNRGGGSGGGGNYRGGFNRSGGGGYSQNRWGNNNRDNNNSNNRGSYNRAPQQQPPPQQ
  PPPPQPPPQQPPPPPSYSPARNPPGASTYNKNSNIPGSSANTSTPTVSSYSPPQPSYSQPPYNQGGYSQG
  YTAPPPPPPPPPAYNYGSYGGYNPAPYTPPPPPTAQTYPQPSYNQYQQYAQQWNQYYQNQGQWPPYYGNY
  DYGSYSGNTQGGTSTQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007040
• ORF Size: 2568 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007040.6
• RefSeq Size: 3892 bp
• RefSeq ORF: 2571 bp
• Locus ID: 11100
• UniProt ID: Q9BUJ2
• Cytogenetics: 19q13.2
• Domains: SAP, SPRY
• Protein Families: Druggable Genome
• MW: 95.8 kDa
• Gene Summary: This gene encodes a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. This protein binds specifically to adenovirus early-1B-55kDa oncoprotein. It may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by early-1B-55kDa in adenovirus-infected cells. [provided by RefSeq, Mar 2016]