RPL14 (NM_003973) Human Tagged ORF Clone

CAT#: RC200425

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RPL14 (Myc-DDK-tagged)-Human ribosomal protein L14 (RPL14), transcript variant 2

  View "NM_003973" in other vectors (10)

USD 98.00

USD 390.00


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    • 10 ug


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Specifications

Product Data
Product Name RPL14 (NM_003973) Human Tagged ORF Clone
Symbol RPL14
Synonyms CAG-ISL-7; CTG-B33; hRL14; L14; RL14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC200425 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGTTCAGGCGCTTCGTGGAGGTTGGCCGGGTGGCCTATGTCTCCTTTGGACCTCATGCCGGAAAAT
TGGTCGCGATTGTAGATGTTATTGATCAGAACAGGGCTTTGGTCGATGGACCTTGCACTCAAGTGAGGAG
ACAGGCCATGCCTTTCAAGTGCATGCAGCTCACTGATTTCATCCTCAAGTTTCCGCACAGTGCCCACCAG
AAGTATGTCCGACAAGCCTGGCAGAAGGCAGACATCAATACAAAATGGGCAGCCACACGATGGGCCAAGA
AGATTGAAGCCAGAGAAAGGAAAGCCAAGATGACAGATTTTGATCGTTTTAAAGTTATGAAGGCAAAGAA
AATGAGGAACAGAATAATCAAGAATGAAGTTAAGAAGCTTCAAAAGGCAGCTCTCCTGAAAGCTTCTCCC
AAAAAAGCACCTGGTACTAAGGGTACTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTGCTAAAGTTCCAG
CAAAAAAGATCACCGCCGCGAGTAAAAAGGCTCCAGCCCAGAAGGTTCCTGCCCAGAAAGCCACAGGCCA
GAAAGCAGCGCCTGCTCCAAAAGCTCAGAAGGGTCAAAAAGCTCCAGCCCAGAAAGCACCTGCTCCAAAG
GCATCTGGCAAGAAAGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200425 protein sequence
Red=Cloning site Green=Tags(s)

MVFRRFVEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSAHQ
KYVRQAWQKADINTKWAATRWAKKIEARERKAKMTDFDRFKVMKAKKMRNRIIKNEVKKLQKAALLKASP
KKAPGTKGTAAAAAAAAAAAKVPAKKITAASKKAPAQKVPAQKATGQKAAPAPKAQKGQKAPAQKAPAPK
ASGKKA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_003973
ORF Size 648 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_003973.2, NM_003973.3, NM_003973.4, NP_003964
RefSeq Size 875
RefSeq ORF 648
Locus ID 9045
Cytogenetics 3p22.1
Domains Ribosomal_L14e, KOW
Protein Pathways Ribosome
MW 23.5 kDa
Gene Summary Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain. The protein is located in the cytoplasm. This gene contains a trinucleotide (GCT) repeat tract whose length is highly polymorphic; these triplet repeats result in a stretch of alanine residues in the encoded protein. Transcript variants utilizing alternative polyA signals and alternative 5'-terminal exons exist but all encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

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