UROS (NM_000375) Human Tagged ORF Clone

CAT#: RC200385

UROS (Myc-DDK-tagged)-Human uroporphyrinogen III synthase (UROS)

Product Images

Circular map for RC200385

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  Western blot validation of overexpression lysate (Cat# LY424759) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200385 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified UROS protein (Cat# TP300385). The protein was produced from HEK293T cells transfected with UROS cDNA clone (Cat# RC200385) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UROS
• Synonyms: UROIIIS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200385 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTTCTTTTACTGAAGGATGCGAAGGAAGATGACTGTGGCCAGGATCCGTATATCAGGGAATTAG
  GATTATATGGACTTGAAGCCACTTTGATCCCTGTTTTATCGTTTGAGTTTTTGTCTCTTCCCAGTTTCTC
  TGAGAAGCTTTCTCATCCTGAAGATTACGGGGGACTCATTTTTACCAGCCCCAGAGCAGTGGAAGCAGCA
  GAGTTATGTTTGGAGCAAAACAATAAAACTGAAGTCTGGGAAAGGTCTCTGAAAGAAAAATGGAATGCCA
  AGTCAGTGTATGTGGTTGGAAATGCTACTGCTTCTCTAGTGAGTAAAATTGGCCTGGATACAGAAGGAGA
  AACCTGTGGAAATGCAGAAAAGCTTGCAGAATATATTTGTTCCAGGGAGTCCTCAGCACTGCCTCTTCTA
  TTTCCCTGTGGAAACCTCAAAAGAGAAATCCTGCCAAAAGCGCTCAAGGACAAAGGGATTGCCATGGAAA
  GCATAACTGTGTATCAGACAGTTGCACACCCAGGAATCCAAGGGAACCTGAACAGCTACTATTCCCAGCA
  GGGGGTTCCAGCCAGCATCACATTTTTTAGTCCCTCTGGCCTCACATACAGTCTCAAGCACATTCAGGAG
  TTATCTGGTGACAATATCGATCAAATTAAGTTTGCAGCCATCGGCCCCACTACGGCTCGCGCGCTGGCCG
  CCCAGGGCCTTCCTGTAAGCTGCACTGCAGAGAGCCCCACGCCACAAGCCCTGGCCACTGGCATCAGGAA
  GGCTCTCCAGCCCCATGGCTGCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200385 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKVLLLKDAKEDDCGQDPYIRELGLYGLEATLIPVLSFEFLSLPSFSEKLSHPEDYGGLIFTSPRAVEAA
  ELCLEQNNKTEVWERSLKEKWNAKSVYVVGNATASLVSKIGLDTEGETCGNAEKLAEYICSRESSALPLL
  FPCGNLKREILPKALKDKGIAMESITVYQTVAHPGIQGNLNSYYSQQGVPASITFFSPSGLTYSLKHIQE
  LSGDNIDQIKFAAIGPTTARALAAQGLPVSCTAESPTPQALATGIRKALQPHGCC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000375
• ORF Size: 795 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000375.3
• RefSeq Size: 1371 bp
• RefSeq ORF: 798 bp
• Locus ID: 7390
• UniProt ID: P10746
• Cytogenetics: 10q26.2
• Domains: HEM4
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Porphyrin and chlorophyll metabolism
• MW: 28.6 kDa
• Gene Summary: The protein encoded by this gene catalyzes the fourth step of porphyrin biosynthesis in the heme biosynthetic pathway. Defects in this gene cause congenital erythropoietic porphyria (Gunther's disease). [provided by RefSeq, Jul 2008]