TFIIF (GTF2F2) (NM_004128) Human Tagged ORF Clone

CAT#: RC200361

GTF2F2 (Myc-DDK-tagged)-Human general transcription factor IIF, polypeptide 2, 30kDa (GTF2F2)

Product Images

Circular map for RC200361

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  Western blot validation of overexpression lysate (Cat# LY418203) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200361 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GTF2F2 protein (Cat# TP300361). The protein was produced from HEK293T cells transfected with GTF2F2 cDNA clone (Cat# RC200361) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TFIIF
• Synonyms: BTF4; RAP30; TF2F2; TFIIF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200361 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGAGCGCGGGGAACTCGACTTGACCGGCGCCAAACAGAACACAGGAGTGTGGCTAGTCAAGGTTC
  CTAAATATTTGTCACAGCAATGGGCTAAAGCTTCTGGAAGAGGTGAAGTTGGGAAACTGCGGATTGCCAA
  GACTCAAGGAAGGACTGAGGTGTCATTTACTTTGAATGAGGATCTTGCAAATATTCATGATATTGGTGGA
  AAACCAGCTTCAGTCAGTGCTCCTAGAGAACATCCATTTGTCTTGCAAAGTGTTGGAGGACAGACATTAA
  CAGTATTTACTGAGAGCTCATCAGATAAGCTGTCATTGGAAGGAATAGTGGTACAAAGAGCTGAATGCCG
  ACCAGCTGCCAGTGAAAACTACATGCGATTAAAAAGATTGCAAATAGAAGAGTCTTCCAAACCAGTGAGG
  CTATCACAACAGCTGGACAAAGTTGTAACAACCAATTACAAACCTGTTGCTAATCATCAATACAATATCG
  AATATGAAAGGAAAAAGAAAGAAGACGGAAAGCGAGCTCGAGCTGATAAACAACATGTTTTAGACATGCT
  ATTTTCAGCCTTTGAGAAACATCAATACTATAATCTTAAGGACTTGGTGGACATCACAAAACAACCTGTG
  GTGTACCTGAAGGAAATCTTAAAAGAAATTGGTGTTCAGAATGTAAAAGGGATCCACAAAAACACATGGG
  AGCTGAAGCCAGAGTACAGACACTATCAAGGAGAAGAAAAGAGTGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200361 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAERGELDLTGAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLRIAKTQGRTEVSFTLNEDLANIHDIGG
  KPASVSAPREHPFVLQSVGGQTLTVFTESSSDKLSLEGIVVQRAECRPAASENYMRLKRLQIEESSKPVR
  LSQQLDKVVTTNYKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPV
  VYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEKSD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004128
• ORF Size: 747 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004128.3
• RefSeq Size: 1469 bp
• RefSeq ORF: 750 bp
• Locus ID: 2963
• UniProt ID: P13984
• Cytogenetics: 13q14.12-q14.13
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• MW: 28.4 kDa
• Gene Summary: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.[UniProtKB/Swiss-Prot Function]