Syntaxin 4 (STX4) (NM_004604) Human Tagged ORF Clone

CAT#: RC200347

STX4 (Myc-DDK-tagged)-Human syntaxin 4 (STX4)

Product Images

Western blot validation of overexpression lysate (Cat# LY401462) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200347 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STX4 protein (Cat# TP300347). The protein was produced from HEK293T cells transfected with STX4 cDNA clone (Cat# RC200347) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syntaxin 4
• Synonyms: p35-2; STX4A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200347 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGACAGGACCCACGAGCTGAGACAGGGGGATGACAGCTCGGACGAAGAGGACAAGGAGCGGGTCG
  CGCTGGTGGTGCACCCGGGCACGGCACGGCTGGGGAGCCCGGACGAGGAGTTCTTCCACAAGGTCCGGAC
  AATTCGGCAGACTATTGTCAAACTGGGGAATAAAGTCCAGGAGTTGGAGAAACAGCAGGTCACCATCCTG
  GCCACGCCCCTTCCCGAGGAGAGCATGAAGCAGGAGCTGCAGAACCTGCGCGATGAGATCAAACAGCTGG
  GGAGGGAGATCCGCCTGCAGCTGAAGGCCATAGAGCCCCAGAAGGAGGAAGCTGATGAGAACTATAACTC
  CGTCAACACAAGAATGAGAAAAACCCAGCATGGGGTCCTGTCCCAGCAATTCGTGGAGCTCATCAACAAG
  TGCAATTCAATGCAGTCCGAATACCGGGAGAAGAACGTGGAGCGGATTCGGAGGCAGCTGAAGATCACCA
  ATGCTGGGATGGTGTCTGATGAGGAGTTGGAGCAGATGCTGGACAGTGGGCAAAGCGAGGTGTTTGTGTC
  CAATATCCTGAAGGACACGCAGGTGACTCGACAGGCCTTAAATGAGATCTCGGCCCGGCACAGTGAGATC
  CAGCAGCTTGAACGCAGTATTCGTGAGCTGCACGACATATTCACTTTTCTGGCTACCGAAGTGGAGATGC
  AGGGGGAGATGATCAATCGGATTGAGAAGAACATCCTGAGCTCAGCGGACTACGTGGAACGTGGGCAGGA
  GCACGTCAAGACGGCCCTGGAGAACCAGAAGAAGGCGAGGAAGAAGAAAGTCTTGATTGCCATCTGTGTG
  TCCATCACCGTCGTCCTCCTAGCAGTCATCATTGGCGTCACAGTGGTTGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200347 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTIL
  ATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINK
  CNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEI
  QQLERSIRELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKKKVLIAICV
  SITVVLLAVIIGVTVVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004604
• ORF Size: 891 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004604.5
• RefSeq Size: 1494 bp
• RefSeq ORF: 894 bp
• Locus ID: 6810
• UniProt ID: Q12846
• Cytogenetics: 16p11.2
• Domains: t_SNARE, SynN
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• MW: 34.2 kDa
• Gene Summary: Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes (By similarity). May also play a role in docking of synaptic vesicles at presynaptic active zones.[UniProtKB/Swiss-Prot Function]