ZNF256 (NM_005773) Human Tagged ORF Clone

CAT#: RC200318

ZNF256 (Myc-DDK-tagged)-Human zinc finger protein 256 (ZNF256)

Product Images

Circular map for RC200318

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF256
• Synonyms: BMZF-3; BMZF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200318 representing NM_005773
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCCGAGCTGACGGCCCCGGCCCAGGGCATTGTGACCTTTGAGGACGTGGCTGTTTACTTCT
  CCTGGAAGGAATGGGGTCTTCTTGATGAGGCTCAGAAATGCCTGTACCACGATGTGATGCTGGAGAACTT
  GACACTTACAACCTCCCTGGGTGGTTCTGGAGCAGGGGATGAGGAGGCACCTTATCAGCAGAGCACTTCT
  CCACAGCGGGTGTCACAGGTTAGGATTCCTAAGGCCCTTCCTTCTCCCCAGAAGACCAACCCCTGTGAGA
  TATGTGGCCCAGTCTTGAGACAGATTTTGCACTTGGTTGAACACCAAGGAACACACCATGGTCAGAAACT
  GTATACAGACGGGGCATGTAGGAAACAATTACAATTTACTGCATACCTTCATCAGCACCAGAAGCAGCAT
  GTTGGACAGAAACACTTCAGAAGCAATGGGGGCAGAGACATGTTTTTGAGCAGCTGCACATTTGAAGTAT
  CTGGGAAGCCCTTCACTTGCAAGGAGGTTGGGAAGGATTTCCTGGTGAGATCAAGATTTCTTCAGCAACA
  GGCTGCTCACACCAGAAAGAAGTCAAACAGAACCAAGAGTGCAGTGGCCTTTCACAGTGTAAAAAATCAT
  TACAACTGGGGAGAATGTGTGAAAGCTTTCAGCTACAAACATGTACGTGTTCAGCACCAGGGAGACCTCA
  TTAGGGAAAGATCTTACATGTGCAGTGAATGTGGGAAATCTTTTAGCACAAGCTGTAGCCTCAGTGATCA
  TTTGAGAGTTCACACTTCAGAAAAGCCTTATACATGTGGAGAATGTGGGAAATCCTATAGGCAAAGCTCT
  AGCCTTATTACGCACCGAAGAATTCACACTGGAGTAAGACCTCATCAATGTGATGAATGTGGAAAATTAT
  TTAACAGGAAGTATGACCTTCTTATACATCAGAGAGTTCATACTGGAGAAAGGCCTTACAAGTGCAGTGA
  ATGTGGGAAATCCTTTAGCCATAGCTCTAGCCTCATTACACACCAGAGAATTCATACTGGAATGAGGCCT
  TATGAGTGCAGTGAATGTGGGAAATCTTTTATCCATAGTTCTAGCCTTATTACACACCAGAGAGTTCACA
  CTGGTACAAGGCCTTATATGTGCAGTGAATGTGGGAAATCCTTTAGCCAGAGCTGTCACCTCATTAAACA
  CCGGAGACTTCACATTGGAGAAGGGCCTTATGAGTGTAGTGAATGTGGGAAATTGTTTACTTATAGATCT
  CGTTTCTTCCAACACCAGAGAGTTCATACTGGAGTAAGATCTCATGAATGTCATGAATGTGGAAAATTAT
  TTAGCAGGAAATTTGACCTCATTGTACATGAGAGAGTTCACACAGGAGAAAGGCCATATGAGTGCAGTGA
  ATGTGGAAAATCCTTTACCTGTAAATCCTACCTCATCTCACACTGGAAAGTTCATACTGGAGCAAGGCCT
  TATGAATGTGGGGAGTGTGGGAAATCATTTACTCATAGCTCTACGCTCCTTCAACACCAGAGAGTTCACA
  CTGGAGAAAGGCCTTATGAGTGCAATGAATGTGGGAAGTTTTTTAGCCAGAGCTCCAGCCTCATTAGACA
  TAGGAGAAGTCACACCGGAGAAAGGCCTTATGAGTGCAGTGAGTGTTGGAAATCCTTTAGTAACCACTCT
  AGCCTCGTTAAACACCGAAGAGTTCATACCGGAGAAAGGCCTTATGAATGCAGTGAATGTGGAAAATCCT
  TTAGCCAGAGCTCTAACCTCACTAATCACCAGCGAATTCACAGTGGGGAAAGGCCTTATGAGTGTAGTGA
  CTGTGGAAAATTTTTTACCTTCAACTCCAACCTCCTAAAACATCAGAACGTTCACAAGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200318 representing NM_005773
  Red=Cloning site Green=Tags(s)
  
  MAAAELTAPAQGIVTFEDVAVYFSWKEWGLLDEAQKCLYHDVMLENLTLTTSLGGSGAGDEEAPYQQSTS
  PQRVSQVRIPKALPSPQKTNPCEICGPVLRQILHLVEHQGTHHGQKLYTDGACRKQLQFTAYLHQHQKQH
  VGQKHFRSNGGRDMFLSSCTFEVSGKPFTCKEVGKDFLVRSRFLQQQAAHTRKKSNRTKSAVAFHSVKNH
  YNWGECVKAFSYKHVRVQHQGDLIRERSYMCSECGKSFSTSCSLSDHLRVHTSEKPYTCGECGKSYRQSS
  SLITHRRIHTGVRPHQCDECGKLFNRKYDLLIHQRVHTGERPYKCSECGKSFSHSSSLITHQRIHTGMRP
  YECSECGKSFIHSSSLITHQRVHTGTRPYMCSECGKSFSQSCHLIKHRRLHIGEGPYECSECGKLFTYRS
  RFFQHQRVHTGVRSHECHECGKLFSRKFDLIVHERVHTGERPYECSECGKSFTCKSYLISHWKVHTGARP
  YECGECGKSFTHSSTLLQHQRVHTGERPYECNECGKFFSQSSSLIRHRRSHTGERPYECSECWKSFSNHS
  SLVKHRRVHTGERPYECSECGKSFSQSSNLTNHQRIHSGERPYECSDCGKFFTFNSNLLKHQNVHKG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005773
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005773.2, NP_005764.2
• RefSeq Size: 2228 bp
• RefSeq ORF: 1884 bp
• Locus ID: 10172
• UniProt ID: Q9Y2P7
• Cytogenetics: 19q13.43
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• MW: 71.7 kDa
• Gene Summary: Transcriptional repressor that plays a role in cell proliferation. Requires TRIM28 for its activity.[UniProtKB/Swiss-Prot Function]