TFIP11 (NM_001008697) Human Tagged ORF Clone

CAT#: RC200290

TFIP11 (Myc-DDK-tagged)-Human tuftelin interacting protein 11 (TFIP11), transcript variant 1

Product Images

Circular map for RC200290

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  Western blot validation of overexpression lysate (Cat# LY423349) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200290 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TFIP11 protein (Cat# TP300290). The protein was produced from HEK293T cells transfected with TFIP11 cDNA clone (Cat# RC200290) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TFIP11
• Synonyms: bK445C9.6; NTR1; Spp382; STIP; STIP-1; TIP39
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200290 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATTGTCCCACTTATACCGGGATGGGGAAGGCCGCATTGATGATGATGATGACGAGCGGGAGAACT
  TTGAGATCACTGACTGGGATCTCCAGAATGAGTTCAACCCCAACCGACAGCGCCACTGGCAGACCAAGGA
  AGAAGCCACCTACGGGGTGTGGGCAGAGCGAGACTCGGATGATGAGAGGCCCAGCTTTGGAGGCAAACGG
  GCCCGTGACTACTCTGCGCCAGTCAACTTCATCAGCGCAGGGCTCAAGAAAGGGGCAGCGGAGGAGGCAG
  AGTTGGAAGATTCTGATGACGAAGAGAAACCTGTTAAGCAGGACGACTTTCCTAAGGATTTTGGACCAAG
  GAAGCTAAAAACGGGTGGCAATTTTAAGCCCAGCCAGAAAGGTTTTGCAGGAGGAACCAAATCTTTCATG
  GACTTCGGCAGCTGGGAAAGACACACAAAAGGAATTGGACAGAAGCTTCTTCAGAAGATGGGCTACGTCC
  CTGGACGGGGCCTCGGGAAGAATGCACAAGGTATCATTAACCCAATTGAAGCCAAGCAGAGAAAGGGAAA
  AGGTGCTGTGGGGGCTTATGGATCCGAGCGCACCACTCAGTCCATGCAAGACTTCCCTGTGGTTGACTCA
  GAGGAAGAAGCTGAAGAGGAGTTTCAGAAGGAGCTGAGCCAGTGGAGGAAAGACCCAAGTGGAAGCAAGA
  AGAAGCCCAAATACTCTTACAAGACCGTGGAAGAGTTGAAGGCCAAGGGCAGGATTAGCAAGAAGCTCAC
  TGCTCCCCAGAAGGAACTTTCTCAAGTCAAGGTCATAGACATGACAGGCCGGGAGCAGAAGGTCTACTAC
  AGCTACAGTCAGATCAGCCACAAGCACAACGTTCCCGATGATGGGCTGCCGCTACAGTCCCAACAGCTGC
  CACAGTCTGGCAAAGAGGCCAAGGCCCCCGGCTTCGCGCTGCCCGAGCTGGAGCACAACCTGCAGCTGCT
  CATCGACCTCACGGAGCAGGAGATCATCCAGAATGACCGGCAGCTACAGTATGAGCGGGACATGGTGGTC
  AACCTCTTCCACGAGCTGGAGAAGATGACCGAGGTCCTGGACCACGAGGAGCGGGTCATCTCGAACCTCA
  GCAAGGTCCTGGAGATGGTGGAGGAGTGCGAGCGGCGGATGCAGCCCGACTGCAGCAACCCCCTCACCCT
  GGACGAGTGTGCCCGCATCTTCGAAACCCTGCAGGACAAGTACTATGAGGAGTACAGGATGTCCGACCGT
  GTGGACCTTGCTGTGGCCATCGTCTATCCACTCATGAAGGAGTACTTCAAGGAGTGGGATCCCCTCAAAG
  ACTGCACTTATGGCACCGAGATCATCTCTAAGTGGAAAAGCCTCCTAGAGAATGACCAGCTCTTGTCCCA
  TGGCGGACAGGACCTCTCAGCAGATGCCTTTCACAGGTTGATATGGGAAGTCTGGATGCCTTTTGTTCGA
  AATATTGTCACCCAGTGGCAGCCAAGGAACTGTGACCCGATGGTGGACTTTTTGGATAGTTGGGTGCACA
  TTATTCCTGTGTGGATCTTAGATAACATACTGGACCAACTCATCTTCCCCAAGCTGCAAAAGGAGGTGGA
  AAACTGGAACCCGCTCACAGACACTGTTCCCATCCACTCTTGGATCCACCCATGGCTGCCCCTTATGCAG
  GCACGGCTGGAGCCACTCTATTCCCCCATCCGTAGTAAGCTGTCCAGCGCCCTGCAGAAGTGGCACCCCA
  GCGACTCCTCTGCCAAGCTCATCCTCCAGCCCTGGAAGGATGTCTTCACTCCTGGCTCCTGGGAAGCATT
  CATGGTCAAAAACATAGTGCCCAAGCTGGGGATGTGTCTTGGTGAGCTAGTCATTAACCCCCACCAGCAG
  CACATGGATGCATTCTATTGGGTGATTGACTGGGAAGGGATGATCTCTGTCTCTAGCCTGGTGGGACTTC
  TTGAAAAGCACTTCTTCCCCAAGTGGCTTCAGGTGCTGTGCTCTTGGCTCAGTAACAGCCCAAATTATGA
  GGAGATCACCAAGTGGTACCTGGGTTGGAAGTCGATGTTCTCAGACCAAGTGCTGGCACATCCATCTGTC
  AAGGACAAATTTAATGAGGCACTTGATATCATGAACCGGGCGGTGTCCTCCAACGTTGGTGCCTACATGC
  AGCCAGGAGCACGGGAGAACATTGCCTATCTCACCCACACGGAGCGGAGGAAGGACTTCCAGTACGAGGC
  CATGCAGGAGAGGCGGGAGGCTGAGAACATGGCTCAGAGGGGCATTGGCGTGGCCGCTAGCTCTGTGCCC
  ATGAACTTTAAGGACCTCATTGAGACCAAGGCTGAGGAGCACAACATTGTCTTCATGCCCGTCATTGGGA
  AGCGACACGAAGGGAAGCAGCTCTACACCTTTGGCCGCATTGTGATCTACATTGACCGGGGAGTGGTCTT
  TGTCCAGGGCGAGAAGACGTGGGTGCCCACCTCACTGCAGAGCCTGATCGACATGGCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200290 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSLSHLYRDGEGRIDDDDDERENFEITDWDLQNEFNPNRQRHWQTKEEATYGVWAERDSDDERPSFGGKR
  ARDYSAPVNFISAGLKKGAAEEAELEDSDDEEKPVKQDDFPKDFGPRKLKTGGNFKPSQKGFAGGTKSFM
  DFGSWERHTKGIGQKLLQKMGYVPGRGLGKNAQGIINPIEAKQRKGKGAVGAYGSERTTQSMQDFPVVDS
  EEEAEEEFQKELSQWRKDPSGSKKKPKYSYKTVEELKAKGRISKKLTAPQKELSQVKVIDMTGREQKVYY
  SYSQISHKHNVPDDGLPLQSQQLPQSGKEAKAPGFALPELEHNLQLLIDLTEQEIIQNDRQLQYERDMVV
  NLFHELEKMTEVLDHEERVISNLSKVLEMVEECERRMQPDCSNPLTLDECARIFETLQDKYYEEYRMSDR
  VDLAVAIVYPLMKEYFKEWDPLKDCTYGTEIISKWKSLLENDQLLSHGGQDLSADAFHRLIWEVWMPFVR
  NIVTQWQPRNCDPMVDFLDSWVHIIPVWILDNILDQLIFPKLQKEVENWNPLTDTVPIHSWIHPWLPLMQ
  ARLEPLYSPIRSKLSSALQKWHPSDSSAKLILQPWKDVFTPGSWEAFMVKNIVPKLGMCLGELVINPHQQ
  HMDAFYWVIDWEGMISVSSLVGLLEKHFFPKWLQVLCSWLSNSPNYEEITKWYLGWKSMFSDQVLAHPSV
  KDKFNEALDIMNRAVSSNVGAYMQPGARENIAYLTHTERRKDFQYEAMQERREAENMAQRGIGVAASSVP
  MNFKDLIETKAEEHNIVFMPVIGKRHEGKQLYTFGRIVIYIDRGVVFVQGEKTWVPTSLQSLIDMAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008697
• ORF Size: 2511 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008697.2
• RefSeq Size: 2941 bp
• RefSeq ORF: 2514 bp
• Locus ID: 24144
• UniProt ID: Q9UBB9
• Cytogenetics: 22q12.1
• MW: 96.8 kDa
• Gene Summary: This gene encodes a protein component of the spliceosome that promotes the release of the lariat-intron during late-stage splicing through the recruitment of a pre-mRNA splicing factor called DEAH-box helicase 15. The encoded protein contains a G-patch domain, a hallmark of RNA-processing proteins, that binds DEAH-box helicase 15. This protein contains an atypical nuclear localization sequence as well as a nuclear speckle-targeting sequence, enabling it to localize to distinct speckled regions within the cell nucleus. Polymorphisms in this gene are associated with dental caries suggesting a role in amelogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]