TSG101 (NM_006292) Human Tagged Lenti ORF Clone

CAT#: RC200275L2

Lenti ORF clone of Human tumor susceptibility gene 101 (TSG101), mGFP tagged

Product Images

Circular map for RC200275L2

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  Double digestion of RC200275L2 using SgfI and MluI

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: mGFP
• Symbol: TSG101
• Synonyms: TSG10; VPS23
• Vector: pLenti-C-mGFP
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC200275).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006292
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006292.2
• RefSeq Size: 1562 bp
• RefSeq ORF: 1173 bp
• Locus ID: 7251
• UniProt ID: Q99816
• Cytogenetics: 11p15.1
• Domains: UBCc
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Endocytosis
• MW: 43.9 kDa
• Gene Summary: The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008]