GPAA1 (NM_003801) Human Tagged ORF Clone

CAT#: RC200271

GPAA1 (Myc-DDK-tagged)-Human glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) (GPAA1)

Product Images

Circular map for RC200271

• 

  Western blot validation of overexpression lysate (Cat# LY418421) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200271 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GPAA1
• Synonyms: GAA1; GPIBD15; hGAA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200271 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCTCCTGTCGGACCCGGTTCGCCGGCGCGCGCTCGCCCGCCTAGTGCTGCGCCTCAACGCGCCGT
  TGTGCGTGCTGAGCTACGTGGCGGGCATCGCCTGGTTCTTGGCGCTGGTTTTCCCGCCGCTGACCCAGCG
  CACTTACATGTCGGAGAACGCCATGGGCTCCACCATGGTGGAGGAGCAGTTTGCGGGCGGAGACCGTGCC
  CGGGCTTTTGCCCGGGACTTCGCCGCCCACCGCAAGAAGTCGGGGGCTCTGCCAGTGGCCTGGCTTGAAC
  GGACGATGCGGTCAGTAGGGCTGGAGGTCTACACGCAGAGTTTCTCCCGGAAACTGCCCTTCCCAGATGA
  GACCCACGAGCGCTATATGGTGTCGGGCACCAACGTGTACGGCATCCTGCGGGCCCCGCGTGCTGCCAGC
  ACCGAGTCGCTTGTGCTCACCGTGCCCTGTGGCTCTGACTCTACCAACAGCCAGGCTGTGGGGCTGCTGC
  TGGCACTGGCTGCCCACTTCCGGGGGCAGATTTATTGGGCCAAAGATATCGTCTTCCTGGTAACAGAACA
  TGACCTTCTGGGCACTGAGGCTTGGCTTGAAGCCTACCACGATGTCAATGTCACTGGCATGCAGTCGTCT
  CCCCTGCAGGGCCGAGCTGGGGCCATTCAGGCAGCCGTGGCCCTGGAGCTGAGCAGTGATGTGGTCACCA
  GCCTCGATGTGGCCGTGGAGGGGCTTAACGGGCAGCTGCCCAACCTTGACCTGCTCAATCTCTTCCAGAC
  CTTCTGCCAGAAAGGGGGCCTGTTGTGCACGCTTCAGGGCAAGCTGCAGCCCGAGGACTGGACATCATTG
  GATGGACCGCTGCAGGGCCTGCAGACACTGCTGCTCATGGTTCTGCGGCAGGCCTCCGGCCGCCCCCACG
  GCTCCCATGGCCTCTTCCTGCGCTACCGTGTGGAGGCCCTAACCCTGCGTGGCATCAATAGCTTCCGCCA
  GTACAAGTATGACCTGGTGGCAGTGGGCAAGGCTTTGGAGGGCATGTTCCGCAAGCTCAACCACCTCCTG
  GAGCGCCTGCACCAGTCCTTCTTCCTCTACTTGCTCCCCGGCCTCTCCCGCTTCGTCTCCATCGGCCTCT
  ACATGCCCGCTGTCGGCTTCTTGCTCCTGGTCCTTGGTCTCAAGGCTCTGGAACTGTGGATGCAGCTGCA
  TGAGGCTGGAATGGGCCTTGAGGAGCCCGGGGGTGCCCCTGGCCCCAGTGTACCCCTTCCCCCATCACAG
  GGTGTGGGGCTGGCCTCGCTCGTGGCACCTCTGCTGATCTCACAGGCCATGGGACTGGCCCTCTATGTCC
  TGCCAGTGCTGGGCCAACACGTTGCCACCCAGCACTTCCCAGTGGCAGAGGCTGAGGCTGTGGTGCTGAC
  ACTGCTGGCGATTTATGCAGCTGGCCTGGCCCTGCCCCACAATACCCACCGGGTGGTAAGCACACAGGCC
  CCAGACAGGGGCTGGATGGCACTGAAGCTGGTAGCCCTGATCTACCTAGCACTGCAGCTGGGCTGCATCG
  CCCTCACCAACTTCTCACTGGGCTTCCTGCTGGCCACCACCATGGTGCCCACTGCTGCGCTTGCCAAGCC
  TCATGGGCCCCGGACCCTCTATGCTGCCCTGCTGGTGCTGACCAGCCCGGCAGCCACGCTCCTTGGCAGC
  CTGTTCCTGTGGCGGGAGCTGCAGGAGGCGCCACTGTCACTGGCCGAGGGCTGGCAGCTCTTCCTGGCAG
  CGCTAGCCCAGGGTGTGCTGGAGCACCACACCTACGGCGCCCTGCTCTTCCCACTGCTGTCCCTGGGCCT
  CTACCCCTGCTGGCTGCTTTTCTGGAATGTGCTCTTCTGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200271 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGLLSDPVRRRALARLVLRLNAPLCVLSYVAGIAWFLALVFPPLTQRTYMSENAMGSTMVEEQFAGGDRA
  RAFARDFAAHRKKSGALPVAWLERTMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAPRAAS
  TESLVLTVPCGSDSTNSQAVGLLLALAAHFRGQIYWAKDIVFLVTEHDLLGTEAWLEAYHDVNVTGMQSS
  PLQGRAGAIQAAVALELSSDVVTSLDVAVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPEDWTSL
  DGPLQGLQTLLLMVLRQASGRPHGSHGLFLRYRVEALTLRGINSFRQYKYDLVAVGKALEGMFRKLNHLL
  ERLHQSFFLYLLPGLSRFVSIGLYMPAVGFLLLVLGLKALELWMQLHEAGMGLEEPGGAPGPSVPLPPSQ
  GVGLASLVAPLLISQAMGLALYVLPVLGQHVATQHFPVAEAEAVVLTLLAIYAAGLALPHNTHRVVSTQA
  PDRGWMALKLVALIYLALQLGCIALTNFSLGFLLATTMVPTAALAKPHGPRTLYAALLVLTSPAATLLGS
  LFLWRELQEAPLSLAEGWQLFLAALAQGVLEHHTYGALLFPLLSLGLYPCWLLFWNVLFWK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003801
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003801.4
• RefSeq Size: 2105 bp
• RefSeq ORF: 1866 bp
• Locus ID: 8733
• UniProt ID: O43292
• Cytogenetics: 8q24.3
• Domains: Gaa1
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• MW: 67.6 kDa
• Gene Summary: Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains. [provided by RefSeq, Jul 2008]