RBPMS (NM_001008712) Human Tagged ORF Clone
RBPMS (Myc-DDK-tagged)-Human RNA binding protein with multiple splicing (RBPMS), transcript variant 3
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC200248 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC200248 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||657 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1366 bp|
|RefSeq ORF||660 bp|
|Protein Families||Stem cell - Pluripotency|
|Gene Summary||This gene encodes a member of the RNA recognition motif family of RNA-binding proteins. The RNA recognition motif is between 80-100 amino acids in length and family members contain one to four copies of the motif. The RNA recognition motif consists of two short stretches of conserved sequence, as well as a few highly conserved hydrophobic residues. The encoded protein has a single, putative RNA recognition motif in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]|
|cDNA Clone Resources|
|RC200248L3||Lenti ORF clone of Human RNA binding protein with multiple splicing (RBPMS), transcript variant 3, Myc-DDK-tagged||
|RC200248L4||Lenti ORF clone of Human RNA binding protein with multiple splicing (RBPMS), transcript variant 3, mGFP tagged||
|RG200248||RBPMS (GFP-tagged) - Human RNA binding protein with multiple splicing (RBPMS), transcript variant 3||
|SC301352||RBPMS (untagged)-Human RNA binding protein with multiple splicing (RBPMS), transcript variant 3||
|SC320655||RBPMS (untagged)-Human RNA binding protein with multiple splicing (RBPMS), transcript variant 3||