RABL4 (IFT27) (NM_006860) Human Tagged ORF Clone

CAT#: RC200215

IFT27 (Myc-DDK-tagged)-Human intraflagellar transport 27 homolog (Chlamydomonas) (IFT27), transcript variant 2

Product Images

Circular map for RC200215

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  Western blot validation of overexpression lysate (Cat# LY416367) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200215 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified IFT27 protein (Cat# TP300215). The protein was produced from HEK293T cells transfected with IFT27 cDNA clone (Cat# RC200215) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RABL4
• Synonyms: BBS19; FAP156; RABL4; RAYL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200215 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGAAGCTGGCAGCCAAATGCATCCTGGCAGGAGACCCAGCAGTGGGCAAGACCGCCCTGGCACAGA
  TCTTCCGCAGTGATGGAGCCCATTTCCAGAAAAGCTACACCCTGACAACAGGAATGGATTTGGTGGTGAA
  GACAGTGCCAGTTCCTGACACGGGAGACAGTGTGGAACTCTTCATTTTTGACTCTGCTGGCAAGGAGCTG
  TTTTCGGAAATGCTGGATAAATTGTGGGAGAGTCCCAATGTCTTATGTCTCGTCTATGATGTGACCAATG
  AAGAATCCTTCAACAACTGCAGCAAGTGGCTGGAGAAGGCTCGGTCACAGGCTCCAGGCATCTCTCTCCC
  AGGTGTTTTAGTTGGGAACAAGACAGACCTGGCCGGCAGACGAGCAGTGGACTCAGCTGAGGCCCGGGCA
  TGGGCGCTGGGCCAGGGCCTGGAATGTTTTGAAACATCCGTGAAAGAGATGGAAAACTTCGAAGCCCCTT
  TCCACTGCCTTGCCAAGCAGTTCCACCAGCTGTACCGGGAGAAGGTGGAGGTTTTCCGGGCCCTGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200215 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVKLAAKCILAGDPAVGKTALAQIFRSDGAHFQKSYTLTTGMDLVVKTVPVPDTGDSVELFIFDSAGKEL
  FSEMLDKLWESPNVLCLVYDVTNEESFNNCSKWLEKARSQAPGISLPGVLVGNKTDLAGRRAVDSAEARA
  WALGQGLECFETSVKEMENFEAPFHCLAKQFHQLYREKVEVFRALA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006860
• ORF Size: 558 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006860.5
• RefSeq Size: 1101 bp
• RefSeq ORF: 558 bp
• Locus ID: 11020
• UniProt ID: Q9BW83
• Cytogenetics: 22q12.3
• Domains: ras, RAS, RHO, RAB
• Protein Families: Druggable Genome
• MW: 20.5 kDa
• Gene Summary: This gene encodes a GTP-binding protein that is a core component of the intraflagellar transport complex B. Characterization of the similar Chlamydomonas protein indicates a function in cell cycle control. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]