MSTO1 (NM_018116) Human Tagged ORF Clone

CAT#: RC200136

MSTO1 (Myc-DDK-tagged)-Human misato homolog 1 (Drosophila) (MSTO1)

Product Images

Circular map for RC200136

• 

  Western blot validation of overexpression lysate (Cat# LY413303) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200136 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MSTO1
• Synonyms: LST005; MMYAT; MST
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200136 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGGGGCCCGGGAGGTGCTCACACTGCAGTTGGGACATTTTGCCGGTTTCGTGGGCGCGCACT
  GGTGGAACCAGCAGGATGCTGCGCTGGGCCGAGCGACCGATTCCAAGGAGCCCCCGGGAGAGCTGTGCCC
  CGACGTCCTGTATCGTACGGGCCGGACGCTGCACGGCCAGGAGACCTACACGCCGCGACTCATCCTCATG
  GATCTGAAGGGTAGTTTGAGCTCCCTAAAAGAGGAAGGTGGACTCTACAGGGACAAACAGTTGGATGCTG
  CAATAGCATGGCAGGGGAAGCTCACCACACACAAAGAGGAACTCTATCCCAAGAACCCTTATCTCCAAGA
  CTTTCTGAGTGCAGAGGGAGTGCTGAGTAGTGATGGTGTCTGGAGGGTCAAATCCATTCCCAATGGCAAA
  GGTTCCTCACCACTCCCCACCGCTACAACTCCAAAACCACTTATCCCTACAGAGGCCAGCATCAGGGTCT
  GGTCAGACTTCCTCAGAGTCCATCTCCATCCCCGGAGCATCTGTATGATTCAGAAGTACAACCACGATGG
  GGAAGCAGGTCGGCTGGAGGCTTTTGGCCAAGGGGAAAGTGTCCTAAAGGAACCCAAGTACCAGGAAGAG
  CTGGAGGACAGGCTGCATTTCTACGTGGAGGAATGTGACTACTTGCAGGGCTTCCAGATCCTGTGTGACC
  TGCACGATGGCTTCTCTGGGGTAGGCGCGAAGGCGGCAGAGCTGCTACAAGATGAATATTCAGGGCGGGG
  AATAATAACCTGGGGCCTGCTACCTGGTCCCTACCATCGTGGGGAGGCCCAGAGAAACATCTATCGTCTA
  TTAAACACAGCTTTTGGTCTCGTGCACCTGACTGCTCACAGCTCTCTTGTCTGCCCCTTGTCCTTGGGTG
  GGAGCCTGGGCCTGCGACCCGAGCCACCTGTCAGCTTCCCTTACCTGCATTATGATGCCACTCTGCCCTT
  CCACTGCAGTGCCATCCTGGCTACAGCCCTGGACACAGTCACTGTTCCTTATCGCCTGTGTTCCTCTCCA
  GTTTCCATGGTTCATCTGGCTGACATGCTGAGCTTCTGTGGGAAAAAGGTGGTGACAGCAGGAGCAATCA
  TCCCTTTCCCCTTGGCTCCAGGCCAGTCCCTTCCTGATTCCCTGATGCAGTTTGGAGGAGCCACCCCATG
  GACCCCACTGTCTGCATGTGGGGAGCCTTCTGGAACACGTTGCTTTGCCCAGTCAGTGGTGCTGAGGGGT
  ATAGACAGAGCATGCCACACAAGCCAGCTCACCCCAGGGACACCTCCACCCTCTGCCCTTCATGCATGTA
  CCACTGGGGAAGAAATCTTGGCTCAGTATTTACAACAGCAGCAGCCTGGAGTCATGAGTTCTTCCCATCT
  GCTGCTGACTCCCTGCAGGGTGGCTCCTCCTTACCCCCACCTCTTCTCAAGCTGCAGTCCACCGGGTATG
  GTTCTGGATGGTTCCCCCAAGGGAGCAGCAGTGGAGAGCATCCCAGTGTTTGGGGCACTGTGTTCCTCTT
  CGTCCCTGCACCAGACCCTGGAAGCCTTGGCCAGAGACCTCACCAAACTCGACTTGCGGCGCTGGGCCAG
  CTTCATGGATGCTGGAGTGGAGCACGATGACGTAGCAGAGCTGCTGCAGGAGCTACAAAGCCTGGCCCAG
  TGCTACCAGGGTGGTGACAGCCTCGTGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200136 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGGAREVLTLQLGHFAGFVGAHWWNQQDAALGRATDSKEPPGELCPDVLYRTGRTLHGQETYTPRLILM
  DLKGSLSSLKEEGGLYRDKQLDAAIAWQGKLTTHKEELYPKNPYLQDFLSAEGVLSSDGVWRVKSIPNGK
  GSSPLPTATTPKPLIPTEASIRVWSDFLRVHLHPRSICMIQKYNHDGEAGRLEAFGQGESVLKEPKYQEE
  LEDRLHFYVEECDYLQGFQILCDLHDGFSGVGAKAAELLQDEYSGRGIITWGLLPGPYHRGEAQRNIYRL
  LNTAFGLVHLTAHSSLVCPLSLGGSLGLRPEPPVSFPYLHYDATLPFHCSAILATALDTVTVPYRLCSSP
  VSMVHLADMLSFCGKKVVTAGAIIPFPLAPGQSLPDSLMQFGGATPWTPLSACGEPSGTRCFAQSVVLRG
  IDRACHTSQLTPGTPPPSALHACTTGEEILAQYLQQQQPGVMSSSHLLLTPCRVAPPYPHLFSSCSPPGM
  VLDGSPKGAAVESIPVFGALCSSSSLHQTLEALARDLTKLDLRRWASFMDAGVEHDDVAELLQELQSLAQ
  CYQGGDSLVD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018116
• ORF Size: 1710 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018116.4
• RefSeq Size: 2500 bp
• RefSeq ORF: 1713 bp
• Locus ID: 55154
• UniProt ID: Q9BUK6
• Cytogenetics: 1q22
• MW: 61.8 kDa
• Gene Summary: Involved in the regulation of mitochondrial distribution and morphology (PubMed:17349998, PubMed:28554942, PubMed:28544275). Required for mitochondrial fusion and mitochondrial network formation (PubMed:28554942, PubMed:28544275).[UniProtKB/Swiss-Prot Function]