IFT122 (NM_052990) Human Tagged ORF Clone

CAT#: RC200119

IFT122 (Myc-DDK-tagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 4

Product Images

Circular map for RC200119

• 

  Western blot validation of overexpression lysate (Cat# LY409347) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200119 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: IFT122
• Synonyms: CED; CED1; FAP80; SPG; WDR10; WDR10p; WDR140
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200119 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGCCGTGTTGACGTGGAGAGATAAAGCCGAGCACTGTATAAATGACATCGCATTTAAGCCTGATG
  GAACTCAACTGATTTTGGCTGCCGGAAGCAGATTACTGGTTTATGACACCTCTGATGGCACCTTACTTCA
  GCCCCTCAAGGGACACAAAGACACTGTGTACTGTGTGGCATATGCGAAGGATGGGTTGTGGTCTCCTGAA
  CAGAAGTCTGTCTCCAAACACAAATCAAGCAGCAAGATCATCTGCTGCAGCTGGACAAATGATGGTCAGT
  ACCTGGCGCTGGGGATGTTCAATGGGATCATCAGCATACGGAACAAAAATGGCGAGGAGAAAGTAAAGAT
  CGAGCGGCCGGGGGGCTCCCTCTCGCCAATATGGTCCATCTGCTGGAACCCTTCAAGAGAGGAACGTAAT
  GACATCCTGGCTGTGGCTGACTGGGGACAGAAAGTTTCCTTCTACCAGCTGAGTGGAAAACAGATTGGAA
  AGGATCGGGCACTGAACTTTGACCCCTGCTGCATCAGCTACTTTACTAAAGGCGAGTACATTTTGCTGGG
  GGGTTCAGACAAGCAAGTATCTCTTTTCACCAAGGATGGAGTGCGGCTTGGGACTGTTGGGGAGCAGAAC
  TCCTGGGTGTGGACGTGTCAAGCGAAACCGGATTCCAACTATGTGGTGGTCGGCTGCCAGGACGGCACCA
  TTTCCTTCTACCAGCTTATTTTCAGCACAGTCCATGGGCTTTACAAGGACCGCTATGCCTACAGGGATAG
  CATGACTGACGTCATTGTGCAGCACCTGATCACTGAGCAGAAAGTTCGGATTAAATGCAAAGAGCTTGTC
  AAGAAGATTGCCATCTACAGAAATCGATTGGCTATCCAACTGCCAGAGAAAATCCTCATCTATGAGTTGT
  ATTCAGAGGACTTATCAGACATGCATTACCGGGTAAAGGAGAAGATTATCAAGAAGTTTGAGTGCAACCT
  CCTGGTGGTGTGTGCCAATCACATCATCCTGTGCCAGGAGAAACGGCTGCAGTGCCTGTCCTTCAGCGGA
  GTGAAGGAGCGGGAGTGGCAGATGGAGTCTCTCATTCGTTACATCAAGGTGATCGGTGGCCCTCCTGGAA
  GAGAAGGCCTCTTAGTGGGGCTGAAGAATGGACAGATCCTGAAGATCTTCGTGGACAATCTCTTTGCTAT
  CGTCCTGCTGAAGCAGGCCACAGCTGTGCGCTGCTTGGACATGAGTGCCTCCCGTAAGAAGCTGGCCGTG
  GTAGATGAAAATGACACTTGCCTGGTGTATGACATCGACACCAAGGAGCTGCTTTTTCAGGAACCAAACG
  CCAACAGTGTAGCTTGGAACACCCAGTGTGAGGACATGCTCTGCTTCTCGGGAGGAGGCTACCTCAACAT
  CAAAGCCAGCACCTTCCCTGTGCACCGGCAGAAGCTGCAGGGCTTTGTGGTCGGCTACAATGGCTCCAAG
  ATCTTCTGCCTCCATGTCTTCTCCATTTCTGCCGTGGAGGTGCCGCAGTCCGCTCCCATGTACCAGTACC
  TGGATAGGAAACTGTTCAAGGAAGCCTACCAGATTGCTTGCTTGGGTGTCACAGACACTGATTGGCGTGA
  ACTGGCCATGGAAGCGCTAGAAGGTTTAGATTTTGAAACTGCAAAGAAGGCCTTCATCAGAGTACAAGAC
  CTCCGATATTTAGAGCTCATCAGCAGCATTGAGGAGAGGAAGAAGCGGGGAGAGACCAACAATGACCTGT
  TTCTGGCAGATGTGTTTTCCTACCAGGGGAAGTTCCATGAGGCCGCCAAACTGTACAAGAGGAGTGGGCA
  CGAGAACCTCGCGCTTGAAATGTACACCGACCTCTGCATGTTTGAGTATGCCAAGGATTTCCTTGGATCT
  GGAGACCCCAAAGAAACAAAGATGCTAATCACCAAACAGGCTGACTGGGCCAGAAATATCAAGGAGCCCA
  AAGCCGCCGTGGAGATGTACATCTCAGCAGGAGAGCACGTCAAGGCCATCGAGATCTGTGGTGACCATGG
  CTGGGTTGACATGTTGATCGACATCGCCCGCAAACTGGACAAGGCTGAGCGCGAGCCCCTGCTGCTGTGC
  GCTACCTACCTCAAGAAGCTGGACAGCCCTGGCTATGCTGCTGAGACCTACCTGAAGATGGGTGACCTCA
  AGTCCCTGGTGCAGCTGCACGTGGAGACCCAGCGCTGGGATGAGGCCTTTGCTTTGGGTGAGAAGCATCC
  TGAGTTTAAGGATGACATCTACATGCCGTATGCTCAGTGGCTAGCAGAGAACGATCGCTTTGAGGAAGCC
  CAGAAAGCGTTCCACAAGGCTGGGCGACAGAGAGAAGCGGTCCAGGTGCTGGAGCAGCTCACAAACAATG
  CCGTGGCGGAGAGCAGGTTTAATGATGCTGCCTATTATTACTGGATGCTGTCCATGCAGTGCCTCGATAT
  AGCTCAAGCAGATCCTGCCCAGAAGGACACAATGCTTGGCAAGTTCTACCACTTCCAGCGTTTGGCAGAG
  CTGTACCATGGTTACCATGCCATCCATCGCCACACGGAAGATCCGTTCAGTGTCCATCGTCCTGAAACTC
  TTTTCAACATCTCCAGGTTCCTGCTGCACAGCCTGCCCAAGGACACCCCCTCGGGCATCTCTAAAGTGAA
  AATACTCTTCACCTTGGCCAAGCAGAGCAAGGCCCTCGGTGCCTACAGGCTGGCCCGGCACGCCTATGAC
  AAGCTGCGTGGCCTGTACATCCCTGCCAGATTCCAAAAGTCCATTGAGCTGGGTACCCTGACCATCCGCG
  CCAAGCCCTTCCACGACAGTGAGGAGTTGGTGCCCTTGTGCTACCGCTGCTCCACCAACAACCCGCTGCT
  CAACAACCTGGGCAACGTCTGCATCAACTGCCGCCAGCCCTTCATCTTCTCCGCCTCTTCCTACGACGTG
  CTACACCTGGTTGAGTTCTACCTGGAGGAAGGGATCACTGATGAAGAAGCCATCTCCCTCATCGACCTGG
  AGGTGCTGAGACCCAAGCGGGATGACAGACAGCTAGAGATTGCAAACAACAGCTCCCAGATTCTGCGGCT
  AGTGGAGACCAAGGACTCCATCGGAGATGAGGACCCGTTCACAGCTAAGCTGAGCTTTGAGCAAGGTGGC
  TCAGAGTTCGTGCCAGTGGTGGTGAGCCGGCTGGTGCTGCGCTCCATGAGCCGCCGGGATGTCCTCATCA
  AGCGATGGCCCCCACCCCTGAGGTGGCAATACTTCCGCTCACTGCTGCCTGACGCCTCCATTACCATGTG
  CCCCTCCTGCTTCCAGATGTTCCATTCTGAGGACTATGAGTTGCTGGTGCTTCAGCATGGCTGCTGCCCC
  TACTGCCGCAGGTGCAAGGATGACCCTGGCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200119 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRAVLTWRDKAEHCINDIAFKPDGTQLILAAGSRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGLWSPE
  QKSVSKHKSSSKIICCSWTNDGQYLALGMFNGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSREERN
  DILAVADWGQKVSFYQLSGKQIGKDRALNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGTVGEQN
  SWVWTCQAKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELV
  KKIAIYRNRLAIQLPEKILIYELYSEDLSDMHYRVKEKIIKKFECNLLVVCANHIILCQEKRLQCLSFSG
  VKEREWQMESLIRYIKVIGGPPGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRKKLAV
  VDENDTCLVYDIDTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHRQKLQGFVVGYNGSK
  IFCLHVFSISAVEVPQSAPMYQYLDRKLFKEAYQIACLGVTDTDWRELAMEALEGLDFETAKKAFIRVQD
  LRYLELISSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALEMYTDLCMFEYAKDFLGS
  GDPKETKMLITKQADWARNIKEPKAAVEMYISAGEHVKAIEICGDHGWVDMLIDIARKLDKAEREPLLLC
  ATYLKKLDSPGYAAETYLKMGDLKSLVQLHVETQRWDEAFALGEKHPEFKDDIYMPYAQWLAENDRFEEA
  QKAFHKAGRQREAVQVLEQLTNNAVAESRFNDAAYYYWMLSMQCLDIAQADPAQKDTMLGKFYHFQRLAE
  LYHGYHAIHRHTEDPFSVHRPETLFNISRFLLHSLPKDTPSGISKVKILFTLAKQSKALGAYRLARHAYD
  KLRGLYIPARFQKSIELGTLTIRAKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFIFSASSYDV
  LHLVEFYLEEGITDEEAISLIDLEVLRPKRDDRQLEIANNSSQILRLVETKDSIGDEDPFTAKLSFEQGG
  SEFVPVVVSRLVLRSMSRRDVLIKRWPPPLRWQYFRSLLPDASITMCPSCFQMFHSEDYELLVLQHGCCP
  YCRRCKDDPGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052990
• ORF Size: 3393 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052990.3
• RefSeq Size: 3933 bp
• RefSeq ORF: 3396 bp
• Locus ID: 55764
• UniProt ID: Q9HBG6
• Cytogenetics: 3q21.3-q22.1
• Domains: WD40
• MW: 129.4 kDa
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This cytoplasmic protein contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation. Mutations in this gene cause cranioectodermal dysplasia-1. A related pseudogene is located on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]