GAL4 (LGALS4) (NM_006149) Human Tagged ORF Clone

CAT#: RC200026

LGALS4 (Myc-DDK-tagged)-Human lectin, galactoside-binding, soluble, 4 (LGALS4)

Product Images

Circular map for RC200026

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  Western blot validation of overexpression lysate (Cat# LY416836) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200026 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LGALS4 protein (Cat# TP300026). The protein was produced from HEK293T cells transfected with LGALS4 cDNA clone (Cat# RC200026) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GAL4
• Synonyms: GAL4; L36LBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200026 representing NM_006149
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTATGTCCCCGCACCGGGCTACCAGCCCACCTACAACCCGACGCTGCCTTACTACCAGCCCATCC
  CGGGCGGGCTCAACGTGGGAATGTCTGTTTACATCCAAGGAGTGGCCAGCGAGCACATGAAGCGGTTCTT
  CGTGAACTTTGTGGTTGGGCAGGATCCGGGCTCAGACGTCGCCTTCCACTTCAATCCGCGGTTTGACGGC
  TGGGACAAGGTGGTCTTCAACACGTTGCAGGGCGGGAAGTGGGGCAGCGAGGAGAGGAAGAGGAGCATGC
  CCTTCAAAAAGGGTGCCGCCTTTGAGCTGGTCTTCATAGTCCTGGCTGAGCACTACAAGGTGGTGGTAAA
  TGGAAATCCCTTCTATGAGTACGGGCACCGGCTTCCCCTACAGATGGTCACCCACCTGCAAGTGGATGGG
  GATCTGCAACTTCAATCAATCAACTTCATCGGAGGCCAGCCCCTCCGGCCCCAGGGACCCCCGATGATGC
  CACCTTACCCTGGTCCCGGACATTGCCATCAACAGCTGAACAGCCTGCCCACCATGGAAGGACCCCCAAC
  CTTCAACCCGCCTGTGCCATATTTCGGGAGGCTGCAAGGAGGGCTCACAGCTCGAAGAACCATCATCATC
  AAGGGCTATGTGCCTCCCACAGGCAAGAGCTTTGCTATCAACTTCAAGGTGGGCTCCTCAGGGGACATAG
  CTCTGCACATTAATCCCCGCATGGGCAACGGTACCGTGGTCCGGAACAGCCTTCTGAATGGCTCGTGGGG
  ATCCGAGGAGAAGAAGATCACCCACAACCCATTTGGTCCCGGACAGTTCTTTGATCTGTCCATTCGCTGT
  GGCTTGGATCGCTTCAAGGTTTACGCCAATGGCCAGCACCTCTTTGACTTTGCCCATCGCCTCTCGGCCT
  TCCAGAGGGTGGACACATTGGAAATCCAGGGTGATGTCACCTTGTCCTATGTCCAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200026 representing NM_006149
  Red=Cloning site Green=Tags(s)
  
  MAYVPAPGYQPTYNPTLPYYQPIPGGLNVGMSVYIQGVASEHMKRFFVNFVVGQDPGSDVAFHFNPRFDG
  WDKVVFNTLQGGKWGSEERKRSMPFKKGAAFELVFIVLAEHYKVVVNGNPFYEYGHRLPLQMVTHLQVDG
  DLQLQSINFIGGQPLRPQGPPMMPPYPGPGHCHQQLNSLPTMEGPPTFNPPVPYFGRLQGGLTARRTIII
  KGYVPPTGKSFAINFKVGSSGDIALHINPRMGNGTVVRNSLLNGSWGSEEKKITHNPFGPGQFFDLSIRC
  GLDRFKVYANGQHLFDFAHRLSAFQRVDTLEIQGDVTLSYVQI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006149
• ORF Size: 969 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006149.4
• RefSeq Size: 1117 bp
• RefSeq ORF: 972 bp
• Locus ID: 3960
• UniProt ID: P56470
• Cytogenetics: 19q13.2
• MW: 35.8 kDa
• Gene Summary: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer. [provided by RefSeq, Jul 2008]