Myo18a (NM_001291213) Mouse Tagged ORF Clone

CAT#: MR231913

Myo18a (myc-DDK-tagged) - Mouse myosin XVIIIA (Myo18a), transcript variant 1

Product Images

Circular map for MR231913

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myo18a
• Synonyms: MAJN; MyoPDZ; MysPDZ; SP-R210
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231913 representing NM_001291213
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTAACCTCATGAAGAAAGATAAGGACAAAGATGGCGGGCGGAAGGAGAAGAAGGAAAAAAAGGAGA
  AGAAGGAGAGGATGTCTGCGGCAGAACTGCGGAGCCTGGAAGAGATGAGCATGCGCCGTGGCTTTTTCAA
  CTTGAACCGGTCCTCCAAGCGGGAATCTAAGACGCGCCTGGAAATCTCCAACCCCATCCCTATCAAGGTG
  GCCAGTGGCTCTGACCTGCACCTGACGGACATTGACTCTGACAGCAATCGGGGTAGCATCATCCTGGACT
  CAGGACACCTAAGCACAGCCAGCTCCAGTGATGACCTCAAGGGTGAAGAAGGCAGCTTCCGGGGCTCTGT
  GCTGCAGCGGGCAGCCAAGTTCGGTTCACTGGCCAAGCAGAACTCTCAGATGATTGTCAAACGTTTTTCC
  TTCTCTCAACGGAGCCGGGATGAGAGTGCCTCGGAGACCTCAACCCCCTCAGAGCACTCTGCAGCCCCAT
  CACCTCAGGTAGAGGTGCGGACGCTTGAGGGACAGCTAATGCAGCATCCTGGGCTAGGCATTCCTCGACC
  AGGACCACGGTCTCGAGTCCCTGAGCTGGTGACTAAAAGATTCCCAGCTGATCTACGTCTTCCCGCTCTG
  GTGCCACCACCACCTCCTGCTCTCCGGGAGCTGGAGTTACAACGAAGACCCACAGGAGACTTTGGCTTCT
  CACTGCGGCGCACAACCATGCTGGATCGGGCCCCTGAGGGTCAGGCTTATCGACGGGTGGTTCACTTTGC
  TGAGCCAGGGGCAGGCACCAAGGACCTGGCCTTGGGGCTGGTACCAGGAGATCGACTGGTGGAGATTAAT
  GGACAGAATGTGGAGAATAAATCCAGGGATGAAATTGTGGAGATGATCCGGCAGTCTGGGGACAGTGTGC
  GGCTCAAGGTACAGCCCATCCCAGAGCTTAGCGAGCTGAGCCGGAGCTGGCTTCGGACTGGCGAGGGACA
  CCGCAGGGAGCCGGCTGATCTGGACCCCGAGGCCGCCTCTCCTGCCTACAGCCAGGTAGGGTGCTCCTGC
  CCCACCCAGGAGCCCTCTGCTTTCTCCAGTGACAACCCACCACCCCCACCCTGTACCTGGGCTGGCGTCT
  GTGCTCGGGCCTCCTGCTGGAGTGCCAAGACGGAAGAGCAGATTGCCGCTGAAGAGGCCTGGTATGAGAC
  GGAGAAGGTGTGGCTGGTCCATAGAGATGGATTCTCCTTAGCCAGCCAGCTCAAGTCTGAGGAGCTCAGC
  CTACCCGAGGGGAAGGCGCGTGTGAAGCTGGACCATGACGGAGCCATCCTGGATGTGGATGAAGATGATA
  TAGAGAAGGCTAATGCTCCTTCATGTGACCGTCTAGAAGATCTGGCCTCACTGGTATACCTCAACGAGTC
  TAGCGTCCTGCATACACTGCGCCAGCGCTATGGGGCTAGCCTGCTACACACCTATGCTGGCCCCAGCCTG
  CTTGTCCTTAGCACCCGAGGGGCTCCTGCTGTGTATTCAGAGAAGGTGATGCACATGTTCAAGGGGTGTC
  GGCGGGAGGACATGGCACCCCACATCTACGCTGTAGCCCAGACTGCATATAGGGCGATGTTGATGAGTCG
  TCAGGACCAGTCCATTGTCCTTCTGGGTAGTAGTGGCAGTGGCAAGACTACCAGCTTTCAGCATCTGGTG
  CAGTATCTGGCTACCATCGCGGGTACCAGTGGGACCAAGGTGTTCTCAGTGGAGAAGTGGCAGGCTCTGT
  CCACCCTCCTGGAAGCCTTTGGGAACAGCCCCACCATCATGAATGGCAGTGCCACCCGCTTCTCCCAGAT
  CCTCTCCCTGGACTTTGACCAAGCTGGCCAGGTGGCTTCGGCCTCCATCCAGACAATGCTCCTGGAGAAG
  CTGCGTGTGGCCCGGCGCCCAGCCAGTGAGGCTACTTTCAATGTCTTCTACTACTTGCTGGCCTGTGGGG
  ATGCCACCCTCAGGACAGAGCTCCACCTGAACCACTTGGCAGAGAACAATGTGTTTGGAATTGTGCCACT
  GTCCAAGCCTGAGGAGAAGCAGAAGGCTGCACAGCAGTTCAGTAAGCTCCAGGCTGCCATGAAGGTGCTG
  GCCATCTCCCCCGAGGAACAGAAGACCTGCTGGCTCATCCTGGCTTCCATCTACCACCTGGGGGCTGCTG
  GAGCTACCAAAGAAGCTGCTGAAGCTGGACGAAAGCAGTTTGCCCGCCACGAATGGGCCCAGAAGGCAGC
  ATACCTGCTGGGCTGCAGCTTGGAAGAGCTGTCCTCGGCTATCTTCAAACACCAGCTAAAAGGAGGCACC
  CTGCAACGGTCCACCTCCTTCCGTCAGGGCCCTGAGGAGAGCGGCCTGGGAGAGGGCACCAAACTGAGTG
  CTCTGGAGTGCTTAGAGGGTATGGCGTCTGGCCTCTACAGTGAGCTCTTTACTCTCCTCATCTCCCTGGT
  AAACAGGGCTCTCAAGTCCAGCCAGCACTCACTCTGTTCCATGATGATCGTGGACACACCAGGCTTCCAG
  AACCCTGAATGGGGCGGCTCGGCCCGTGGAGCCTCCTTCGAGGAGCTGTGCCACAACTATGCCCAGGACA
  GGCTGCAGAGGCTGTTCCACGAGCGCACCTTCCTCCAGGAGTTGGAGAGATACAAGGAGGATAACATAGA
  ACTGGCGTTTGATGACTTGGAGCCAGTTGCAGATGACTCCGTGGCTGCTGTGGACCAGGCTTCCCACCTG
  GTTCGCTCACTGGCCCATGCCGATGAGGCAAGAGGGCTACTGTGGCTCCTGGAAGAGGAGGCACTGGTGC
  CTGGTGCGACAGAGGATGCCCTCCTTGATCGTCTTTTCTCCTATTATGGCCCCCAGGAGGGTGACAAAAA
  AGGCCAGAGTCCTCTTCTGCGAAGTAGCAAACCTCGGCACTTTCTCCTGGGCCACAGCCACGGTACCAAC
  TGGGTGGAGTACAACGTGGCTGGCTGGCTGAATTACACCAAGCAGAACCCAGCCACCCAGAATGCACCCC
  GGCTTCTGCAGGACTCCCAGAAAAAGATCATCAGTAACCTGTTTCTGGGCCGGGCGGGCAGTGCCACGGT
  GCTCTCAGGCTCGATTGCAGGCCTGGAAGGTGGCTCCCAGCTGGCCTTGCGCCGGGCAACCAGCATGAGG
  AAAACCTTCACCACCGGCATGGCCGCTGTCAAGAAGAAGTCGCTGTGCATACAGATTAAGCTGCAAGTAG
  ATGCCCTCATTGATACCATCAAGAGGTCCAAGATGCATTTCGTGCACTGCTTCCTTCCTGTGGCCGAGGG
  CTGGCCTGGGGAGCCCCGGTCTGCCTCTTCCCGCAGAGTCAGCAGCAGCAGTGAGCTGGACCTGCCCCCG
  GGAGACCCCTGTGAGGCTGGGCTGCTACAACTGGATGTGTCCCTGCTCCGAGCCCAGCTCCGGGGATCCC
  GCCTGCTTGATGCGATGCGCATGTACCGCCAAGGTTACCCAGACCACATGGTGTTTTCTGAGTTCCGCCG
  GCGCTTTGATGTCCTGGCTCCACACCTGACCAAGAAACATGGTCGTAACTACATCGTGGTGGATGAGAAG
  CGGGCTGTGGAAGAGCTGCTGGAGTCCCTGGACCTGGAAAAGAGCAGCTGCTGTCTGGGCCTAAGCCGGG
  TGTTCTTCCGGGCAGGCACGTTGGCACGGTTGGAAGAACAACGAGACGAGCAAACCAGCAGGCACCTGAC
  CCTGTTCCAGGCAGCCTGCCGGGGCTACCTTGCCCGCCAGCACTTCAAGAAGAGAAAGATCCAGGACCTG
  GCCATTCGCTGTGTGCAGAAGAACATCAAGAAGAACAAGGGGGTGAAGGACTGGCCCTGGTGGAAGCTCT
  TCACCACCGTGCGGCCCCTCATCCAAGTTCAGCTGTCAGAGGAGCAGATCCGAAACAAAGACGAGGAGAT
  CCAGCAGCTGCGCAGCAAGCTTGAGAAGGTGGAGAAGGAGAGGAACGAGCTCCGACTCAGCAGTGACCGG
  CTAGAGACCCGGATCTCAGAACTGACATCGGAGCTGACTGATGAACGCAACACGGGAGAGTCCGCCTCCC
  AGCTGCTGGACGCGGAGACAGCCGAGAGGCTCCGGACCGAGAAGGAGATGAAAGAGCTACAGACCCAGTA
  TGACGCACTGAAGAAGCAGATGGAGGTGATGGAGATGGAAGTGATGGAAGCCCGGCTCATTCGGGCAGCC
  GAGATCAACGGGGAGGTGGATGATGATGATGCAGGGGGAGAGTGGAGGCTGAAGTACGAGCGAGCTGTTC
  GGGAGGTGGACTTCACCAAGAAGCGGCTCCAGCAGGAGCTGGAAGACAAGATGGAGGTGGAACAGCAGAG
  CAGGAGGCAGCTGGAGAGGCGGCTTGGGGACCTGCAAGCAGATAGTGATGAGAGTCAACGGGCACTGCAG
  CAGCTCAAGAAGAAGTGCCAAAGGCTCACAGCTGAGCTGCAGGACACCAAGCTGCACCTGGAGGGCCAGC
  AGGTCCGAAACCACGAGCTGGAGAAGAAGCAGAGGAGGTTCGACAGTGAGCTTTCCCAGGCCCACGAGGA
  GACGCAGCGGGAGAAGCTGCAGAGGGAGAAACTCCAGCGGGAGAAGGACATGCTCCTGGCTGAGGCTTTC
  AGCTTGAAGCAGCAAATGGAGGAAAAAGACTTGGACATTGCGGGGTTTACCCAGAAGGTGGTTTCCTTGG
  AGGCTGAGCTTCAGGACATTTCTTCTCAAGAGTCTAAGGATGAGGCTTCTCTGGCCAAGGTCAAGAAGCA
  GCTCCGGGACTTGGAGGCCAAGGTCAAGGACCAGGAAGAGGAGCTGGATGAGCAGGCTGGGAGTATACAG
  ATGCTGGAGCAGGCCAAGCTGCGTCTGGAGATGGAGATGGAGAGAATGAGACAGACCCATTCCAAGGAGA
  TGGAGAGTCGGGATGAGGAGGTGGAAGAGGCCCGGCAGTCATGTCAGAAGAAGTTAAAACAGATGGAAGT
  GCAGCTCGAGGAGGAGTATGAAGACAAGCAGAAGGCGCTGCGGGAGAAACGGGAGCTGGAGAGCAAGCTC
  TCCACGCTCAGTGACCAGGTGAACCAGCGGGACTTTGAATCAGAGAAGCGGCTACGGAAAGACCTGAAGC
  GCACCAAAGCGCTGCTGGCAGATGCCCAGATCATGCTGGACCACTTAAAGAACAATGCCCCGAGCAAGAG
  GGAGATTGCCCAGCTGAAGAACCAGCTGGAAGAGTCGGAATTCACCTGTGCAGCAGCTGTAAAGGCACGG
  AAAGCGATGGAGGTGGAGATGGAAGACCTGCACCTGCAGATTGATGACATCGCCAAAGCCAAGACAGCGC
  TGGAGGAGCAACTGAGTCGACTTCAGCGTGAGAAAAACGAGATTCAAAACCGGCTGGAAGAGGATCAGGA
  GGACATGAATGAGCTGATGAAGAAGCACAAGGCAGCTGTGGCCCAGGCCTCCCGGGACATGGCACAGATG
  AACGATCTCCAGGCTCAGATTGAAGAGTCCAACAAGGAGAAGCAAGAGCTACAGGAGAAGCTACAAGCTC
  TGCAGAGCCAGGTGGAGTTCCTGGAGCAGTCCATGGTGGACAAGTCCCTCGTCAGCAGGCAGGAAGCGAA
  GATCAGGGAGCTGGAGACACGCCTGGAGTTCGAAAAGACCCAAGTGAAGCGCCTGGAGAACCTGGCCAGT
  CGGCTCAAAGAAACCATGGAGAAGCTGACTGAGGAACGGGACCAGCGCGCCGCAGCTGAGAACCGTGAGA
  AGGAGCAGAACAAGAGGCTCCAGCGACAGCTTCGTGACACCAAGGAGGAGATGAGCGAGCTTGCCAGGAA
  GGAAGCCGAGGCTAGCCGCAAGAAGCATGAACTGGAGATGGACCTGGAGAGCCTGGAAGCTGCTAACCAA
  AGCCTGCAAGCCGACCTAAAGCTGGCGTTCAAACGCATTGGGGACTTGCAAGCTGCCATTGAGGATGAGA
  TGGAAAGTGACGAGAACGAGGACCTCATCAACAGTGAGGGGGACTCAGATGTGGACTCAGAGCTGGAGGA
  CCGGGTCGACGGGGTCAAGTCCTGGTTGTCAAAGAACAAGGGACCTTCTAAGGCACCTTCTGACGATGGC
  AGCTTGAAGAGTTCCAGCCCAACCAGCCACTGGAAGCCACTCGCCCCTGACCCATCGGATGATGAGCATG
  ATCCTGTGGACAGCATCTCCAGACCCCGGTTCTCCCACAGCTATCTGAGTGACAGCGACACAGAGGCCAA
  GCTGACAGAGACCAGTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231913 representing NM_001291213
  Red=Cloning site Green=Tags(s)
  
  MFNLMKKDKDKDGGRKEKKEKKEKKERMSAAELRSLEEMSMRRGFFNLNRSSKRESKTRLEISNPIPIKV
  ASGSDLHLTDIDSDSNRGSIILDSGHLSTASSSDDLKGEEGSFRGSVLQRAAKFGSLAKQNSQMIVKRFS
  FSQRSRDESASETSTPSEHSAAPSPQVEVRTLEGQLMQHPGLGIPRPGPRSRVPELVTKRFPADLRLPAL
  VPPPPPALRELELQRRPTGDFGFSLRRTTMLDRAPEGQAYRRVVHFAEPGAGTKDLALGLVPGDRLVEIN
  GQNVENKSRDEIVEMIRQSGDSVRLKVQPIPELSELSRSWLRTGEGHRREPADLDPEAASPAYSQVGCSC
  PTQEPSAFSSDNPPPPPCTWAGVCARASCWSAKTEEQIAAEEAWYETEKVWLVHRDGFSLASQLKSEELS
  LPEGKARVKLDHDGAILDVDEDDIEKANAPSCDRLEDLASLVYLNESSVLHTLRQRYGASLLHTYAGPSL
  LVLSTRGAPAVYSEKVMHMFKGCRREDMAPHIYAVAQTAYRAMLMSRQDQSIVLLGSSGSGKTTSFQHLV
  QYLATIAGTSGTKVFSVEKWQALSTLLEAFGNSPTIMNGSATRFSQILSLDFDQAGQVASASIQTMLLEK
  LRVARRPASEATFNVFYYLLACGDATLRTELHLNHLAENNVFGIVPLSKPEEKQKAAQQFSKLQAAMKVL
  AISPEEQKTCWLILASIYHLGAAGATKEAAEAGRKQFARHEWAQKAAYLLGCSLEELSSAIFKHQLKGGT
  LQRSTSFRQGPEESGLGEGTKLSALECLEGMASGLYSELFTLLISLVNRALKSSQHSLCSMMIVDTPGFQ
  NPEWGGSARGASFEELCHNYAQDRLQRLFHERTFLQELERYKEDNIELAFDDLEPVADDSVAAVDQASHL
  VRSLAHADEARGLLWLLEEEALVPGATEDALLDRLFSYYGPQEGDKKGQSPLLRSSKPRHFLLGHSHGTN
  WVEYNVAGWLNYTKQNPATQNAPRLLQDSQKKIISNLFLGRAGSATVLSGSIAGLEGGSQLALRRATSMR
  KTFTTGMAAVKKKSLCIQIKLQVDALIDTIKRSKMHFVHCFLPVAEGWPGEPRSASSRRVSSSSELDLPP
  GDPCEAGLLQLDVSLLRAQLRGSRLLDAMRMYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRNYIVVDEK
  RAVEELLESLDLEKSSCCLGLSRVFFRAGTLARLEEQRDEQTSRHLTLFQAACRGYLARQHFKKRKIQDL
  AIRCVQKNIKKNKGVKDWPWWKLFTTVRPLIQVQLSEEQIRNKDEEIQQLRSKLEKVEKERNELRLSSDR
  LETRISELTSELTDERNTGESASQLLDAETAERLRTEKEMKELQTQYDALKKQMEVMEMEVMEARLIRAA
  EINGEVDDDDAGGEWRLKYERAVREVDFTKKRLQQELEDKMEVEQQSRRQLERRLGDLQADSDESQRALQ
  QLKKKCQRLTAELQDTKLHLEGQQVRNHELEKKQRRFDSELSQAHEETQREKLQREKLQREKDMLLAEAF
  SLKQQMEEKDLDIAGFTQKVVSLEAELQDISSQESKDEASLAKVKKQLRDLEAKVKDQEEELDEQAGSIQ
  MLEQAKLRLEMEMERMRQTHSKEMESRDEEVEEARQSCQKKLKQMEVQLEEEYEDKQKALREKRELESKL
  STLSDQVNQRDFESEKRLRKDLKRTKALLADAQIMLDHLKNNAPSKREIAQLKNQLEESEFTCAAAVKAR
  KAMEVEMEDLHLQIDDIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDMAQM
  NDLQAQIEESNKEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQEAKIRELETRLEFEKTQVKRLENLAS
  RLKETMEKLTEERDQRAAAENREKEQNKRLQRQLRDTKEEMSELARKEAEASRKKHELEMDLESLEAANQ
  SLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSEGDSDVDSELEDRVDGVKSWLSKNKGPSKAPSDDG
  SLKSSSPTSHWKPLAPDPSDDEHDPVDSISRPRFSHSYLSDSDTEAKLTETSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291213
• ORF Size: 6249 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291213.1, NP_001278142.1
• RefSeq Size: 7504 bp
• RefSeq ORF: 6252 bp
• Locus ID: 360013
• UniProt ID: Q9JMH9
• Cytogenetics: 11 B5
• MW: 236.3 kDa
• Gene Summary: May link Golgi membranes to the cytoskeleton and participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus (PubMed:19837035). Alternatively, in concert with LURAP1 and CDC42BPA/CDC42BPB, has been involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (By similarity). May be involved in the maintenance of the stromal cell architectures required for cell to cell contact (PubMed:10733906). Regulates trafficking, expression, and activation of innate immune receptors on macrophages. Plays a role to suppress inflammatory responsiveness of macrophages via a mechanism that modulates CD14 trafficking (PubMed:25965346). Acts as a receptor of surfactant-associated protein A (SFTPA1/SP-A) and plays an important role in internalization and clearance of SFTPA1-opsonized S.aureus by alveolar macrophages (PubMed:21123169). Strongly enhances natural killer cell cytotoxicity (By similarity).[UniProtKB/Swiss-Prot Function]