Ank1 (NM_001277281) Mouse Tagged ORF Clone

CAT#: MR231886

Ank1 (myc-DDK-tagged) - Mouse ankyrin 1, erythroid (Ank1), transcript variant 4

Product Images

Circular map for MR231886

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Ank1
• Synonyms: Ank-1; nb; pale
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231886 representing NM_001277281
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTTCTGTAAAGCCGATGCTGCTACCAGCTTTCTGCGGGCGGCACGCTCGGGGAACCTGGACAAGG
  CTCTGGATCACCTGCGCAATGGAGTGGACATTAACACCTGTAACCAGAACGGGTTGAACGGCCTGCATCT
  GGCCTCCAAAGAAGGCCATGTGAAGATGGTGGTTGAACTTCTGCACAAAGAGATCATTCTAGAAACGACA
  ACCAAGAAGGGGAACACTGCTCTGCACATCGCTGCCCTTGCTGGTCAGGATGAGGTGGTCCGGGAGCTGG
  TCAACTATGGAGCCAATGTCAATGCCCAGTCTCAGAAAGGCTTTACTCCCCTGTACATGGCTGCTCAGGA
  GAACCACTTGGAAGTGGTGAAATTTCTACTGGAGAATGGAGCCAATCAGAATGTAGCCACAGAAGATGGC
  TTCACCCCACTGGCCGTGGCTCTACAGCAGGGTCACGAGAATGTGGTGGCTCACCTCATCAACTATGGGA
  CGAAAGGGAAAGTGCGCCTCCCTGCCCTGCACATCGCGGCCCGCAACGATGACACACGGACAGCCGCAGT
  CCTTCTGCAGAATGACCCCAACCCAGATGTGCTTTCCAAGACGGGATTCACACCCCTCCACATCGCAGCT
  CACTATGAGAACCTCAACGTGGCCCAGTTGCTCCTCAACAGGGGAGCCAGCGTCAACTTCACACCTCAGA
  ATGGCATCACCCCACTACACATCGCCTCCCGCAGGGGGAACGTGATCATGGTGAGACTCCTGCTGGACCG
  AGGGGCTCAGATAGAAACGAGGACCAAGGATGAATTGACACCGCTCCACTGTGCAGCTCGGAATGGACAC
  GTGAGAATCTCAGAGATCCTGCTGGACCACGGGGCACCCATCCAAGCCAAAACCAAGAATGGCTTGTCCC
  CAATCCACATGGCCGCTCAGGGAGACCACCTCGACTGTGTCCGACTTCTATTGCAATACAATGCAGAGAT
  AGACGACATCACCTTGGATCACCTGACTCCTCTCCATGTGGCAGCCCACTGTGGCCACCACCGGGTGGCT
  AAGGTTCTTTTGGATAAAGGGGCCAAGCCCAACTCCAGAGCCCTGAATGGTTTTACCCCGTTACACATCG
  CCTGCAAGAAGAACCACATCCGTGTAATGGAGTTGCTGCTGAAGACAGGAGCCTCCATCGACGCGGTCAC
  TGAGTCTGGCCTGACACCTCTCCACGTAGCCTCCTTCATGGGACACCTTCCTATTGTGAAGAACTTACTG
  CAGCGGGGAGCGTCACCCAATGTCTCCAATGTGAAAGTAGAAACCCCCTTGCACATGGCAGCCCGAGCAG
  GGCATACAGAAGTGGCCAAATATTTGCTCCAGAACAAAGCCAAAGCCAACGCCAAGGCCAAGGATGACCA
  GACACCGCTTCACTGTGCTGCTCGAATCGGCCACACAGGCATGGTGAAGCTCCTGCTGGAGAATGGTGCC
  AGCCCCAATCTGGCTACCACTGCTGGCCACACACCCCTACACACCGCAGCCCGTGAGGGACACGTGGACA
  CAGCCCTGGCCCTGCTGGAGAAGGAGGCATCCCAAGCCTGCATGACCAAGAAAGGATTTACCCCTCTGCA
  CGTGGCTGCTAAGTACGGGAAGGTACGGTTGGCGGAGCTGCTGCTGGAACACGATGCACACCCCAATGCA
  GCTGGGAAGAACGGCTTGACTCCTCTGCATGTGGCCGTCCATCACAACAACCTGGACATTGTCAAACTTC
  TTCTTCCCCGAGGTGGCTCCCCCCACAGCCCTGCCTGGAATGGCTACACTCCTTTGCACATCGCTGCCAA
  GCAGAACCAGATAGAGGTGGCCCGCAGTCTACTGCAGTACGGAGGGTCAGCGAATGCAGAGTCGGTACAA
  GGCGTGACCCCACTTCACCTGGCTGCCCAAGAGGGCCACACAGAAATGGTTGCTCTTCTCCTGTCCAAGC
  AAGCCAACGGCAACCTGGGGAACAAGAGTGGACTCACTCCCCTCCACCTGGTGTCACAAGAAGGCCATGT
  TCCAGTGGCAGACGTGCTGATCAAACATGGCGTCACCGTGGATGCTACCACCCGGATGGGTTACACCCCG
  CTCCATGTGGCCAGTCATTATGGAAACATCAAGCTGGTGAAGTTCTTGCTACAGCACCAGGCAGATGTCA
  ATGCCAAGACCAAGCTAGGATACAGCCCCTTGCACCAGGCAGCTCAACAAGGACACACAGACATTGTGAC
  ATTGCTCCTGAAGAATGGTGCTTCTCCAAATGAGGTCAGCTCGAACGGAACCACACCTCTGGCAATAGCC
  AAACGTTTGGGCTACATCTCTGTAACAGACGTGCTCAAGGTGGTCACAGATGAAACCAGTGTGGTGTTAG
  TCAGTGACAAACACCGGATGAGCTACCCTGAGACGGTGGACGAGATCCTGGACGTGTCTGAAGATGAAGG
  TGACGAACTTGTCGGCTCGAAGGCTGAAAGGCGGGACTCCCGGGATGTGGGTGAGGAGAAAGAGCTGTTG
  GATTTTGTGCCGAAGCTAGACCAAGTGGTGGAGTCTCCAGCCATCCCAAGGATCCCTTGTGTCACACCTG
  AGACCGTTGTGATCCGATCTGAAGATCAGGAACAAGCATCTAAAGAATATGATGAGGATTCCCTCATTCC
  CAGCAGCCCGGCCACAGAGACCTCAGACAACATTAGCCCTGTGGCTAGCCCGGTCCACACAGGGTTCCTG
  GTGAGCTTCATGGTGGATGCCCGAGGGGGATCCATGAGAGGAAGCCGTCATAATGGCCTCAGGGTGGTGA
  TCCCTCCCCGGACATGCGCAGCACCCACTCGCATCACCTGCCGCCTCGTTAAGCCGCAGAAGCTGAACAC
  GCCACCCCCACTGGCTGAGGAAGAGGGCCTAGCCAGCAGGATCATTGCCCTGGGACCCACGGGGGCCCAG
  TTCCTTAGCCCTGTGATCGTGGAAATCCCCCATTTTGCCTCCCATGGCCGTGGGGACCGTGAGCTGGTGG
  TTCTGAGGAGTGAAAACGGCTCTGTGTGGAAGGAGCACAAGAGTCGTTATGGGGAAAGCTACCTGGACCA
  GATCCTTAATGGCATGGATGAAGAGCTGGGGAGCCTGGAGGAGCTGGAGAAGAAACGTGTGTGCCGCATC
  ATCACCACCGACTTCCCTCTGTACTTTGTCATCATGTCCCGGCTCTGCCAGGACTACGACACCATCGGTC
  CTGAAGGGGGTTCCCTGAGAAGCAAGCTGGTACCGCTGGTACAGGCAACGTTCCCTGAAAACGCTGTGAC
  CAAGAAAGTGAAGCTGGCTCTGCAGGCCCAGCCTGTCCCGGATGAGCTGGTGACCAAGCTCCTGGGTAAC
  CAGGCCACATTCAGCCCCATTGTGACCGTGGAGCCGAGGCGTCGGAAGTTCCACCGTCCCATCGGGCTGC
  GAATCCCGCTCCCTCCCTCATGGACGGACAACCCCAGGGACAGTGGGGAGGGAGACACCACCAGCCTGCG
  CCTACTGTGTAGCGTCATCGGTGGAACTGACCAAGCCCAGTGGGAAGATATAACGGGAACAACCAAACTT
  ATATACGCCAATGAGTGTGCCAACTTTACCACCAATGTCTCAGCCAGGTTCTGGCTGTCAGACTGTCCCC
  GGACTGCGGAGGCTGTGCACTTTGCCACTCTCCTGTACAAAGAGCTCACAGCAGTCCCCTACATGGCTAA
  GTTTGTCATTTTTGCCAAGATGAACGACGCTCGGGAAGGACGGCTCCGTTGCTACTGCATGACGGATGAC
  AAAGTGGACAAGACCCTGGAGCAGCATGAAAACTTCGTGGAGGTGGCCCGGAGCAGGGACATAGAGGTTC
  TGGAAGGGATGCCTCTGTTTGCAGAACTCTCTGGGAACTTGGTTCCTGTCAAGAAAGCGGCCCAACAGCG
  GAGCTTCCATTTCCAGTCGTTTCGAGAGAATCGTCTGGCCATCCCCGTGAAGGTGAGGGACAGCAGCCGC
  GAGCCAGGAGGGTTCTTGTCGTTCCTACGAAAGACAATGAAGTACGAGGACACACAGCACATCCTCTGTC
  ACCTGAATATCACCATGCCCCCCTGCACCAAGGGCAGTGGAGCAGAAGACAGGAGAAGGACCCTGACACC
  CCTGACCCTTCGATACAGCATTCTCAGCGAGTCGCGGCTGGGTTTTACCAGTGACACAGACCGTGTCGAA
  ATGAGGATGGCTGTCATCAGAGAACACCTCGGCCTAAGCTGGGCAGAGCTGGCCCGGGAGCTGCAGTTCA
  GCGTGGAAGACATCAACCGGATCCGTGTAGAAAACCCTAACTCCTTGTTGGACCAGAGTACAGCCTTGCT
  GACCCTCTGGGTGGACCGTGAAGGCGAAAATGCAAAGATGGAGAATTTGTACACAGCCCTGCGGAACATC
  GACAGGAGTGAGATTGTTAACATGTTGGAGGGCTCCGGCAGGCAGAGCAGAAACCTCAAACCAGAGCGGA
  GACATGGGGACCGGGAGTACTCATTGTCACCCTCCCAGGTGAATGGTTACTCCTCGCTGCAGGACGAGCT
  GCTGTCCCCCGCCTCCCTGCAGTACGCTCTCCCCTCTCCACTGTGTGCAGACCAGTACTGGAACGAAGTG
  GCCGTCATAGACGCCATCCCCCTGGCGGCTACAGAGCATGACACCATGCTGGAGATGTCTGACATGCAGG
  TGTGGTCTGCGGGCCTCACACCCTCCCTGGTCACTGCTGAGGACTCCTCTCTGGAGTGCAGCAAGGCTGA
  GGACTCTGACGCCATACCAGAGTGGAAGTTGGAAGGGGCACACTCAGAGGACACGCAGGGCCCGGAGCTG
  GGCTCTCAGGACCTGGTGGAGGACGACACAGTGGATTCAGATGCCACAAATGGCCTGGCAGATTTGCTAG
  GTCAGGAGGAAGGTCAGAGGTCAGAAAAGAAGAGGCAGGAAGTCTCAGGCACAGAGCAGGACACGGAGAC
  TGAAGTGTCTCTTGTTTCAGGCCAGCAGCGAGTTCACGCCCGAATCACAGACTCACCCTCAGTGAGGCAG
  GTGCTGGACAGAAGCCAGGCCAGAACACTGGACTGGGATAAACAGGGTTCCACAGCGGTACACCCGCAAG
  AAGCCACACAGAGCTCCTGGCAAGAGGAGGTCACGCAGGGCCCACACTCATTCCAGAGAAGGATCACCAC
  CATCCAAGGGCCGGAGCCTGGTGCGCTTCAGGAATACGAGCAGGTGCTGGTGTCTACCAGGGAGCATGTG
  CAGAGGGGGCCACCTGAGACCGGCAGCCCCAAAGCTGGCAAGGAACCTAGCCTGTGGGCACCTGAGAGCG
  CCTTCTCTCAAGAGGTGCAGGGGGATGAGCTTCAGAATATTCCAGGAGAGCAGGTGACGGAGGAACAATT
  CACAGATGAACAGGGCAACATTGTTACCAAGAAGATCATTCGCAAAGTCGTCCGGCAGGTAGACTCGTCT
  GGTGCCATCGACACCCAGCAGCACGAGGAGGTGGAGCTAAGAGGGAGTGGACTCCAGCCGGACCTGATAG
  AGGGCAGGAAGGGGGCTCAGATAGTGAAGCGGGCCAGCCTGAAAAGGGGCAAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231886 representing NM_001277281
  Red=Cloning site Green=Tags(s)
  
  MGFCKADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETT
  TKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDG
  FTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAA
  HYENLNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETRTKDELTPLHCAARNGH
  VRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYNAEIDDITLDHLTPLHVAAHCGHHRVA
  KVLLDKGAKPNSRALNGFTPLHIACKKNHIRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLL
  QRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKANAKAKDDQTPLHCAARIGHTGMVKLLLENGA
  SPNLATTAGHTPLHTAAREGHVDTALALLEKEASQACMTKKGFTPLHVAAKYGKVRLAELLLEHDAHPNA
  AGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQIEVARSLLQYGGSANAESVQ
  GVTPLHLAAQEGHTEMVALLLSKQANGNLGNKSGLTPLHLVSQEGHVPVADVLIKHGVTVDATTRMGYTP
  LHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLLLKNGASPNEVSSNGTTPLAIA
  KRLGYISVTDVLKVVTDETSVVLVSDKHRMSYPETVDEILDVSEDEGDELVGSKAERRDSRDVGEEKELL
  DFVPKLDQVVESPAIPRIPCVTPETVVIRSEDQEQASKEYDEDSLIPSSPATETSDNISPVASPVHTGFL
  VSFMVDARGGSMRGSRHNGLRVVIPPRTCAAPTRITCRLVKPQKLNTPPPLAEEEGLASRIIALGPTGAQ
  FLSPVIVEIPHFASHGRGDRELVVLRSENGSVWKEHKSRYGESYLDQILNGMDEELGSLEELEKKRVCRI
  ITTDFPLYFVIMSRLCQDYDTIGPEGGSLRSKLVPLVQATFPENAVTKKVKLALQAQPVPDELVTKLLGN
  QATFSPIVTVEPRRRKFHRPIGLRIPLPPSWTDNPRDSGEGDTTSLRLLCSVIGGTDQAQWEDITGTTKL
  IYANECANFTTNVSARFWLSDCPRTAEAVHFATLLYKELTAVPYMAKFVIFAKMNDAREGRLRCYCMTDD
  KVDKTLEQHENFVEVARSRDIEVLEGMPLFAELSGNLVPVKKAAQQRSFHFQSFRENRLAIPVKVRDSSR
  EPGGFLSFLRKTMKYEDTQHILCHLNITMPPCTKGSGAEDRRRTLTPLTLRYSILSESRLGFTSDTDRVE
  MRMAVIREHLGLSWAELARELQFSVEDINRIRVENPNSLLDQSTALLTLWVDREGENAKMENLYTALRNI
  DRSEIVNMLEGSGRQSRNLKPERRHGDREYSLSPSQVNGYSSLQDELLSPASLQYALPSPLCADQYWNEV
  AVIDAIPLAATEHDTMLEMSDMQVWSAGLTPSLVTAEDSSLECSKAEDSDAIPEWKLEGAHSEDTQGPEL
  GSQDLVEDDTVDSDATNGLADLLGQEEGQRSEKKRQEVSGTEQDTETEVSLVSGQQRVHARITDSPSVRQ
  VLDRSQARTLDWDKQGSTAVHPQEATQSSWQEEVTQGPHSFQRRITTIQGPEPGALQEYEQVLVSTREHV
  QRGPPETGSPKAGKEPSLWAPESAFSQEVQGDELQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQVDSS
  GAIDTQQHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277281
• ORF Size: 5586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277281.2, NP_001264210.1
• RefSeq Size: 7980 bp
• RefSeq ORF: 5589 bp
• Locus ID: 11733
• UniProt ID: Q02357
• Cytogenetics: 8 11.42 cM
• MW: 204.6 kDa
• Gene Summary: Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu7 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils.[UniProtKB/Swiss-Prot Function]