Neurl4 (NM_001291118) Mouse Tagged ORF Clone

CAT#: MR231841

Neurl4 (myc-DDK-tagged) - Mouse neuralized homolog 4 (Drosophila) (Neurl4), transcript variant 2

Product Images

Circular map for MR231841

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Neurl4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231841 representing NM_001291118
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGGGTCGGGTGGGAGTGGGGGCTCTGGGGCGGGCCCCGGGCCAGGACCGGGGCCCGGCGGGG
  GTGGGGGCCCTGGCAGCAGCGGACCAGGCCTGGGGTCCGGCGGGGGTCTCGGCGGTGGCGGGGAGCTGCA
  CCCGCGCACCGGGCGCTTGGTCAGCCTGTCGGCCTGCGGGCGCACGGCGCGGCGGCAGCAGCCGGGCCAA
  GAGTTTAACCACGGGCTGGTGCTGAGCCGAGAACCCTTGCGCGATGGACGCGTCTTCACCGTCCGCATCG
  ACCGCAAGGTCAACTCCTGGAGTGGCTCCATTGAGATTGGAGTGACAGCACTGGATCCCAGTGTGTTGGA
  CTTCCCGAGCAGTGCCACCGGGCTGAAGGGAGGCTCCTGGGTAGTGTCAGGCTGCTCAGTGCTGCGGGAC
  GGGCGCTCTGTGTTGGAGGAATATGGCCAGGACCTGGACCAGCTTGTCGAAGGGGACCGTGTGGGTGTGG
  AACGCACAGCCACTGGGGAGCTCCGGCTCTGGGTAAACGGGCGGGATTGTGGTGTGGCAGCCACAGGCCT
  GCCTGCTCGTGTCTGGGCTGTTGTGGACCTTTATGGCAAGTGCACCCAGATCACTGTGCTGCCCTCCGAG
  CCAGGCTTCAGCCCTCCCACTCCTGTCCCCACACCTCCCCTTGAGCCCTTGGCCCCTCCAGAAGATTCAG
  CCCTGTTGGAACAGGGGACCTCTGTGGATGAAGCCTTCATGGTGTCCCCAGCGCAGGCACGGCCGGAGAC
  GTTTCCTAACAGCCTTGATTCGCATAATGACTTTGCCAGTATGGAGCTGTCTGAGGTGGTGAGCAACGCC
  ATCCTGTCTGCCTACAATGGGGGCCTCCTTAATGTGAGCCTGAGCTCCCCACCAGCAGGGGACGGACTGG
  CATCCAGTGGGCCAGCCACTTCTCCCATCCTCACTTCCAACGATGCCCTGCTCTTCCATGAGAAGTGTGG
  GACCCTCATCAAACTCAGCAACAACAATAAGACAGCAGAGCGCCGGAGGCCCCTGGATGAATTCAACAAT
  GGAGTTGTCATGACCAACCGCCCACTTCGGGATAATGAAATGTTTGAGATCCGGATCGACAAGCTTGTAG
  ACAAGTGGTCTGGTTCCATCGAGATTGGTGTCACCACCCACAACCCCAATAGCCTGGAGTACCCAGCCAC
  CATGACCAACCTGCAGTCAGGCACCATCATGATGAGTGGCTGTGGGATCCTGACCAACGGCAAAGGCACT
  CGGCGGGAGTACTGCGAGTTCAGTCTGGACGAGCTGCAGGAGGGTGACCATATTGGCCTCACGAGGAAGT
  CCAACTCTGCCCTACACTTCTTCATTAATGGCATCGATCAGGGCGTAGCTACTCCATTGACACCACCAGT
  TGTATATGGTGTAGTAGACTTGTATGGAATGGCAGTGAAAGTGACCATCGTCCATAATAACAACCACAGT
  GACCGTCTCCGCCGGAACAACGCTATCCTGAGGGCTCTGTCCCCCGAGGGTGCTCTTCGTCGTGCTGCCC
  CTGCAGCCCAGGCAGAACCTGAACGCTTGCTCTTCCATCCTAACTGTGGCCAGAAGGCAGCTATCACCCA
  CGAAGGACGCACTGCCCTGAGGCCCCATGCCACTGATGACTTCAATCATGGCGTGGTACTGAGCAGCAGA
  GCCCTCCGGGATGGAGAGGTGTTCCAGGTGCGCATTGACAAGATGGTGGACAAATGGGCTGGCTCCATTG
  AGATTGGTGTCACCACCCATAACCCTGCCTACCTCCAGCTACCCTCTACTATGACCAACTTACGCTCTGG
  GACCTGGATGATGACTGGGAATGGGGTCATGCACAATGGGACAACCATCCTAGATGAATATGGGCACAAC
  CTGGACCGCCTCAAGGCAGGGGACACCGTGGGTGTGGTGCGGCGGGAGGATGGAACGCTCCACTTCTTCG
  TCAATGGGATGACTCAGGGCCCTGCTGCCTGGAATGTCCCCCCGGGGGTCTATGCTGTTGTTGATCTCTA
  CGGCCAGGCTGCCCAGGCTACTATTGTGGATGACGTGGAGGTGCCTCCAGTCTCTGAGCCACTCCCTGAA
  GGAAACAACCAGATGTCTCCCAGTTCTCCATCCTCAGCAGCTGGGGGCTCTGACCTTCGCTTCCACCAGC
  TGCACGGCAGTAATGCAGTCATCACCAATGGTGGCCGCACTGCTCTCCGCCACAACTGCCGCAGCGAGTT
  CAACGATGCCATTGTCATTTCCAACCGAGCCCTGCGGGATGGAGAACTGTTTGAAATTGTCATTCAGAAG
  ATGGTAGATCGTTGGTCAGGCTCCATCGAAGCTGGAGTTACTGCTATTCGGCCGGAAGACCTAGAATTTC
  CCAACACCATGACAGACATTGACTATGATACGTGGATGCTGAGTGGTACAGCTATCATGCAAGATGGCAA
  TACGATGCGCAACAACTATGGGTGTGATCTGGACGCGCTGGGCACAGGTGCACGAATTGGCATGATGCGA
  ACTGCCAAGGGTGATCTGCACTATTTCATTAATGGACAGGACCAAGGCGCTGCCTGCTCAGGCTTGCCTC
  CGGAGGTGTATGCAGTTGTGGATCTCTATGGCCAGTGTGTCCAAGTGTCTATCACCAATGCTACCGGCCC
  CATGGACAACAGCCTGGCCACCAGCAATACTGCCACTGAGAAGTCATTCCCCTTGCATTCCCCAGTGGCG
  GGTGTGGCTCACCGATTCCATAGCATGTGTGGCAAGAATGTCACTCTGGAGGAGGATGGCACACGGGCAG
  TCCGTGTGGCTGGCTACGCACATGGCCTCGTTTTCAGCACCAAAGAGCTCAAGGCTGAAGAAGTTTTTGA
  GGTGAAAGTGGAAGAGCTAGACGAGAAGTGGGCAGGCTCCCTCCGGCTGGGGCTGACCACACTTGCACCA
  GAGGACATGGGGCCTGGAGCGGGCAGTGGTCCAGGGCTGCCTCCTTCCTTGCCTGAACTCCGGACAAAGA
  CCACCTGGATGGTGTCCAGCTGTGAAGTGAGGCGTGATGGGCACCTCCAAAGGATGAACTATGGCCGGAA
  CCTCGAGAGACTAGGGGTGGGAAGCCGTGTGGGCATTCGTCGGTGTGCAGATGACACAATGCACATCTTG
  GTAGATGGAGAAGATATGGGGCCTGCTGCCGCTGGCATTGCCAAGAATGTGTGGGCTGTGTTGGATCTGT
  ATGGGCCAGTACGCAGTGTGGCCATTGTCAGCTCCACAAGGCTGGAGGAACCAGAAGGCACCCAGCCGCC
  TTCTCCCAGCTCAGACACTGGCAGTGAGGTCGAGGAAGATGATGAGGTCGAGGAGCAGGGGCTGAGAGGC
  CAGAATCAAGTGGGGATTGTGCCCACAGCCCTTGAATTCCTGGAGAACCATGGGAAGAATATCCTCTTAT
  CCAACGGGAACCGCACAGCCACTCGGGTGGCCAGCTATAACCAGGGCATCGTTGTCATCAGTCAGCCCCT
  GGTGCCCCATATGCTTGTTCAGGTGCGAATAGACTTCCTGAACCGACAGTGGACATCTTCTCTTGTTCTG
  GGAGTTATCACCTGCCCACCTGAGAGGCTTAACTTCCCTGCCTCTGCTTGTGCCCTTAAACGGGCAGCCT
  GGCTCCTGCGGGGCCGGGGTGTCTTCCACAATGGTCTGAAGATCTGTGAGAAGTTTGGGCCAAATCTTGA
  CACCTGCCCTGAAGGCACCATCCTGGGACTGCGGCTAGACTCCTCTGGAGGGCTTCATCTCCACATCAAT
  GGGGTGGACCAAGGGGTAGCTGTACCAGATGTGCCCCAGCCATGCCATGCACTGGTGGACCTCTATGGAC
  AGTGTGAGCAGGTGACAATTGTGAGCCCTGACCCAGGGACTGCCAGTGGGAAGATTGCTGGAACTCAAGG
  GGACATGGAAAAAGCTGATATGGTAGACGGTATCAAGGAGAGTGTGTGCTGGGGTCCACCGCCTGCTGCT
  AGCCCTTTAAAGAGCTGCGAGTACCATGCCCTTTGCTCCCGCTTCCAAGAACTGCTGTTGCTTCCTGAAG
  ATTATTTCATGCCTCCACCGAAGCGTAGCTTATGCTACTGTGAATCTTGCCGGAAGTTGCGAGGAGATGA
  GGCCCACAGGCGCCGAGGAGAGCCTCCCCGGGAGTACGCCCTGCCTTTCGGATGGTGCAGGTTCAACCTT
  AGGGTGAATCCCCATCTGGAAGCTGGCACACTAACAAAGAAGTGGCACATGGCATATCATGGAAGCAGTG
  TAGCTGTTGTGCGGAGAGTGCTGGACCGAGGGGAGTTGGGAGCAGGTACTACCTCCATCCTGAGCTGCCG
  ACCTTTGAAGGGAGAGCCTGGGGTGGGATTTGAGGAGCCTGGTGAGAACTGTGCACCTCCCCGGGAAGAG
  CAGCCCCCTCCAGTGCTGCTCTCCCCCTCCCTTCAGTATGCCGGGGCAGAGATGCTGGCCTCCAAAGTGC
  AATTCCGGGACCCAAAATCCCAGCGGACACACCAGGCTCAGGTGGCCTTTCAGGTGTGTGTGCGCCCTGG
  CTCCTATACTCCTGGCCCTCCCTCTGCGGCCCTCAGAGAACTTCCTGACCAGCACTTCAGCCCATCGGAA
  CTTGAGTGGGTCACTAAGGAGAAGGGAGCCACACTCCTCTATGCCCTGCTGGTACGAGTGGAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231841 representing NM_001291118
  Red=Cloning site Green=Tags(s)
  
  MAAGSGGSGGSGAGPGPGPGPGGGGGPGSSGPGLGSGGGLGGGGELHPRTGRLVSLSACGRTARRQQPGQ
  EFNHGLVLSREPLRDGRVFTVRIDRKVNSWSGSIEIGVTALDPSVLDFPSSATGLKGGSWVVSGCSVLRD
  GRSVLEEYGQDLDQLVEGDRVGVERTATGELRLWVNGRDCGVAATGLPARVWAVVDLYGKCTQITVLPSE
  PGFSPPTPVPTPPLEPLAPPEDSALLEQGTSVDEAFMVSPAQARPETFPNSLDSHNDFASMELSEVVSNA
  ILSAYNGGLLNVSLSSPPAGDGLASSGPATSPILTSNDALLFHEKCGTLIKLSNNNKTAERRRPLDEFNN
  GVVMTNRPLRDNEMFEIRIDKLVDKWSGSIEIGVTTHNPNSLEYPATMTNLQSGTIMMSGCGILTNGKGT
  RREYCEFSLDELQEGDHIGLTRKSNSALHFFINGIDQGVATPLTPPVVYGVVDLYGMAVKVTIVHNNNHS
  DRLRRNNAILRALSPEGALRRAAPAAQAEPERLLFHPNCGQKAAITHEGRTALRPHATDDFNHGVVLSSR
  ALRDGEVFQVRIDKMVDKWAGSIEIGVTTHNPAYLQLPSTMTNLRSGTWMMTGNGVMHNGTTILDEYGHN
  LDRLKAGDTVGVVRREDGTLHFFVNGMTQGPAAWNVPPGVYAVVDLYGQAAQATIVDDVEVPPVSEPLPE
  GNNQMSPSSPSSAAGGSDLRFHQLHGSNAVITNGGRTALRHNCRSEFNDAIVISNRALRDGELFEIVIQK
  MVDRWSGSIEAGVTAIRPEDLEFPNTMTDIDYDTWMLSGTAIMQDGNTMRNNYGCDLDALGTGARIGMMR
  TAKGDLHYFINGQDQGAACSGLPPEVYAVVDLYGQCVQVSITNATGPMDNSLATSNTATEKSFPLHSPVA
  GVAHRFHSMCGKNVTLEEDGTRAVRVAGYAHGLVFSTKELKAEEVFEVKVEELDEKWAGSLRLGLTTLAP
  EDMGPGAGSGPGLPPSLPELRTKTTWMVSSCEVRRDGHLQRMNYGRNLERLGVGSRVGIRRCADDTMHIL
  VDGEDMGPAAAGIAKNVWAVLDLYGPVRSVAIVSSTRLEEPEGTQPPSPSSDTGSEVEEDDEVEEQGLRG
  QNQVGIVPTALEFLENHGKNILLSNGNRTATRVASYNQGIVVISQPLVPHMLVQVRIDFLNRQWTSSLVL
  GVITCPPERLNFPASACALKRAAWLLRGRGVFHNGLKICEKFGPNLDTCPEGTILGLRLDSSGGLHLHIN
  GVDQGVAVPDVPQPCHALVDLYGQCEQVTIVSPDPGTASGKIAGTQGDMEKADMVDGIKESVCWGPPPAA
  SPLKSCEYHALCSRFQELLLLPEDYFMPPPKRSLCYCESCRKLRGDEAHRRRGEPPREYALPFGWCRFNL
  RVNPHLEAGTLTKKWHMAYHGSSVAVVRRVLDRGELGAGTTSILSCRPLKGEPGVGFEEPGENCAPPREE
  QPPPVLLSPSLQYAGAEMLASKVQFRDPKSQRTHQAQVAFQVCVRPGSYTPGPPSAALRELPDQHFSPSE
  LEWVTKEKGATLLYALLVRVE
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291118
• ORF Size: 4683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291118.1, NP_001278047.1
• RefSeq Size: 5261 bp
• RefSeq ORF: 4686 bp
• Locus ID: 216860
• UniProt ID: Q5NCX5
• Cytogenetics: 11 B3
• MW: 167.9 kDa
• Gene Summary: Promotes CCP110 ubiquitination and proteasome-dependent degradation. By counteracting accumulation of CP110, maintains normal centriolar homeostasis and preventing formation of ectopic microtubular organizing centers (By similarity).[UniProtKB/Swiss-Prot Function]