Slit2 (NM_001291227) Mouse Tagged ORF Clone

CAT#: MR231836

Slit2 (myc-DDK-tagged) - Mouse slit homolog 2 (Drosophila) (Slit2), transcript variant 1

Product Images

Circular map for MR231836

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slit2
• Synonyms: b2b1200.1Clo; Drad; Drad-1; E030015M03Rik; E130320P19Rik; mKIAA4141; S; Slil3; slit-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231836 representing NM_001291227
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGCATTGGCTGGCAGACACTGTCCCTATCGCTGGGGTTAGTGTTGTCGATCTTGAACAAGGTGG
  CGCCGCAGGCGTGCCCGGCCCAGTGCTCCTGTTCAGGCAGCACGGTGGACTGTCATGGGCTGGCACTGCG
  CAGTGTGCCCAGGAATATCCCCCGCAACACCGAGAGACTGGATTTGAATGGAAATAACATCACGAGGATC
  ACGAAGATAGATTTTGCTGGTCTCAGGCACCTCAGAGTTCTTCAGCTCATGGAGAACAGAATCAGCACCA
  TCGAGAGGGGAGCATTCCAGGATCTTAAGGAGCTGGAAAGACTGCGTTTAAACAGAAATAACCTTCAGTT
  GTTTCCTGAGCTGCTGTTTCTCGGGACTGCGAAGCTCTACCGGCTTGATCTCAGTGAAAATCAAATTCAA
  GCAATTCCAAGGAAGGCTTTCCGTGGGGCAGTTGACATTAAAAACCTGCAACTGGATTACAACCAGATCA
  GCTGCATTGAAGATGGGGCGTTCAGAGCTCTACGAGATCTGGAAGTGCTCACTCTGAACAATAACAATAT
  TACTAGACTTTCAGTGGCAAGTTTCAACCATATGCCTAAACTTAGGACATTTCGACTCCACTCGAACAAC
  TTGTACTGCGACTGCCACCTAGCCTGGCTCTCAGACTGGCTTCGCCAAAGGCCACGGGTGGGCTTGTACA
  CTCAGTGTATGGGCCCATCCCACCTGAGGGGCCACAATGTAGCAGAGGTTCAAAAACGAGAGTTTGTCTG
  CAGTGATGAGGAAGAAGGTCACCAGTCATTCATGGCTCCCTCCTGCAGTGTGCTGCACTGCCCCGCTGCT
  TGTACCTGTAGCAACAACATTGTAGACTGCCGAGGGAAAGGTCTCACTGAGATCCCCACAAATCTGCCTG
  AGACCATCACAGAAATACGTTTGGAACAGAACTCCATCAGGGTCATCCCTCCAGGAGCCTTCTCACCATA
  CAAAAAGCTTAGACGACTAGACCTGAGCAACAACCAGATCTCTGAACTTGCACCAGATGCCTTCCAAGGA
  CTGCGCTCTCTGAATTCACTTGTCCTGTATGGAAATAAAATCACAGAACTCCCAAAAAGTTTATTCGAAG
  GACTATTTTCCTTGCAGCTACTATTATTGAATGCCAACAAGATAAACTGCCTTCGGGTAGATGCTTTTCA
  GGACCTGCACAACTTGAACCTTCTCTCCTTATATGACAATAAGCTTCAGACGGTTGCCAAGGGCACCTTC
  TCAGCCCTCAGAGCCATCCAAACTATGCATTTGGCCCAGAATCCTTTCATTTGTGACTGCCATCTCAAGT
  GGCTAGCGGATTATCTCCACACCAACCCAATTGAGACCAGCGGTGCCCGTTGCACCAGCCCCCGCCGCCT
  GGCAAACAAAAGAATTGGACAGATCAAAAGCAAGAAATTCCGTTGTTCAGCTAAAGAACAGTATTTCATT
  CCAGGTACAGAAGATTATCGATCAAAATTAAGTGGAGACTGCTTTGCAGACTTGGCTTGTCCTGAGAAGT
  GTCGCTGTGAAGGGACCACAGTAGACTGCTCCAATCAAAGACTCAACAAAATCCCTGACCATATTCCCCA
  GTACACAGCAGAGCTGCGTCTCAATAATAATGAATTCACAGTGTTAGAAGCCACGGGAATATTTAAGAAA
  CTTCCTCAGTTACGTAAAATCAACTTTAGCAACAATAAGATCACGGATATCGAGGAGGGTGCATTTGAAG
  GCGCGTCTGGTGTGAATGAAATTCTTCTCACCAGTAACCGTTTGGAAAATGTTCAGCATAAGATGTTCAA
  AGGACTGGAGAGCCTCAAAACATTGATGCTGAGAAGTAATCGAATAAGCTGTGTTGGGAACGACAGTTTC
  ATAGGACTCGGCTCTGTGCGTCTGCTCTCTTTATATGACAATCAAATTACCACAGTGGCACCAGGAGCAT
  TTGATTCTCTCCATTCATTATCCACTCTAAACCTCTTGGCCAATCCTTTCAACTGTAACTGTCACCTGGC
  ATGGCTGGGAGAATGGCTCAGAAGGAAAAGAATTGTAACAGGAAATCCTCGATGCCAAAAACCCTACTTC
  CTGAAGGAAATCCCAATCCAGGATGTAGCCATTCAGGACTTCACCTGTGATGATGGAAATGATGACAATA
  GTTGCTCTCCACTCTCCCGTTGTCCTTCTGAATGTACCTGCTTGGATACAGTGGTACGATGTAGCAACAA
  GGGCTTGAAGGTTTTGCCTAAAGGTATTCCAAAAGATGTCACAGAGCTGTATCTGGATGGGAACCAGTTT
  ACGCTGGTCCCGAAGGAACTCTCTAACTACAAACATTTAACACTTATAGACTTAAGTAACAACCGAATAA
  GCACCCTTTCCAATCAAAGCTTCAGCAACATGACCCAGCTTCTCACCTTAATCCTCAGTTACAACCGTCT
  GAGATGTATCCCTCCACGAACCTTTGATGGATTGAAGTCTCTTCGGTTACTGTCTTTACATGGAAATGAC
  ATTTCTGTTGTGCCTGAAGGTGCCTTCAATGACTTGTCAGCCTTGTCACACTTAGCGATTGGAGCCAACC
  CTCTTTACTGTGATTGTAACATGCAGTGGTTATCCGACTGGGTGAAGTCGGAATATAAGGAACCTGGAAT
  TGCACGCTGTGCCGGCCCTGGAGAAATGGCAGATAAATTATTACTCACTACTCCCTCCAAAAAATTTACA
  TGTCAAGGTCCCGTGGATATCACTATTCAAGCCAAGTGTAATCCCTGCTTATCAAATCCATGTAAAAATG
  ATGGCACCTGTAACAATGACCCCGTTGATTTTTATCGATGTACCTGCCCATATGGATTCAAGGGTCAGGA
  CTGTGATGTCCCCATTCATGCTTGTATCAGTAATCCATGTAAACATGGAGGAACTTGTCACTTAAAGGAA
  GGAGAGAATGCTGGATTCTGGTGCACTTGTGCTGATGGGTTTGAAGGAGAAAACTGTGAAGTCAATATTG
  ATGATTGTGAAGATAATGATTGTGAAAATAATTCTACATGCGTTGATGGAATTAACAACTACACATGTCT
  TTGCCCACCGGAATACACAGCTGCTAATCTGAATGAGGTGGAAAAAGGTGAACTGTGTGAGGAAAAGCTG
  GACTTCTGTGCACAAGACTTGAATCCCTGCCAGCATGACTCCAAGTGCATCCTGACTCCAAAGGGATTCA
  AGTGTGACTGCACTCCAGGATACATTGGTGAGCACTGTGACATTGACTTTGATGACTGCCAAGATAACAA
  GTGTAAAAACGGTGCTCACTGCACAGATGCCGTGAACGGATACACGTGCGTCTGTCCTGAAGGCTACAGT
  GGCTTGTTCTGTGAGTTTTCTCCACCCATGGTCCTCCCTCGCACCAGCCCCTGTGATAATTTTGATTGCC
  AGAATGGAGCCCAGTGTATCATCAGGATAAATGAACCAATATGCCAGTGTTTGCCTGGCTACCTGGGAGA
  GAAGTGTGAGAAATTGGTCAGTGTGAATTTTGTAAACAAAGAGTCCTATCTTCAGATTCCTTCAGCCAAG
  GTTCGGCCTCAGACAAACATCACACTTCAGATTGCCACAGATGAAGACAGCGGCATCCTCTTGTATAAAG
  GTGACAAAGACCACATTGCCGTGGAACTCTATAGAGGGCGAGTTCGAGCCAGCTATGACACCGGCTCTCA
  TCCGGCTTCTGCCATTTACAGTGTGGAGACAATCAATGATGGAAACTTCCACATTGTGGAGCTACTGACC
  CTGGATTCCAGTCTTTCCCTCTCTGTGGATGGAGGAAGCCCTAAAGTCATCACCAATTTGTCAAAACAAT
  CTACTCTGAATTTCGACTCTCCACTCTATGTAGGAGGCATGCCTGGGAAAAATAACGTGGCATCCCTGCG
  CCAGGCCCCTGGGCAAAATGGCACCAGCTTCCATGGCTGTATCCGGAACCTTTACATTAACAGTGAGCTG
  CAGGACTTCCGGAAAATGCCTATGCAAACCGGAATTCTGCCTGGCTGTGAACCATGCCACAAGAAAGTAT
  GTGCCCATGGCATGTGCCAGCCCAGCAGCCAATCAGGCTTCACCTGTGAATGTGAGGAAGGGTGGATGGG
  GCCCCTCTGTGACCAGAGAACCAATGATCCCTGCCTCGGAAACAAATGTGTGCATGGGACCTGCCTGCCC
  ATCAATGCCTTCTCCTATAGTTGCAAGTGCCTGGAGGGCCATGGCGGTGTCCTCTGTGATGAAGAAGAAG
  ATCTCTTTAACCCCTGCCAGATGATCAAGTGCAAGCATGGGAAGTGCAGGCTTTCTGGAGTGGGCCAGCC
  CTATTGTGAATGCAACAGTGGATTCACCGGGGACAGCTGTGATAGAGAAATTTCTTGTCGAGGGGAACGG
  ATAAGGGACTATTACCAGAAGCAGCAGGGTTACGCTGCCTGTCAAACAACTAAGAAAGTATCTCGCTTGG
  AATGCAGAGGCGGGTGCGCTGGAGGCCAGTGCTGTGGACCTCTGAGAAGCAAGAGGCGGAAATACTCTTT
  CGAATGCACAGATGGCTCCTCATTTGTGGACGAGGTTGAGAAAGTGGTGAAGTGCGGCTGCGCGAGATGT
  GCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231836 representing NM_001291227
  Red=Cloning site Green=Tags(s)
  
  MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRI
  TKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQ
  AIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNN
  LYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSDEEEGHQSFMAPSCSVLHCPAA
  CTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQG
  LRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTF
  SALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFI
  PGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKK
  LPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSF
  IGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYF
  LKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQF
  TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGND
  ISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFT
  CQGPVDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKE
  GENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTAANLNEVEKGELCEEKL
  DFCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYS
  GLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAK
  VRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLT
  LDSSLSLSVDGGSPKVITNLSKQSTLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSEL
  QDFRKMPMQTGILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCLP
  INAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREISCRGER
  IRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKYSFECTDGSSFVDEVEKVVKCGCARC
  AS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001291227
• ORF Size: 4626 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291227.2, NP_001278156.1
• RefSeq Size: 8555 bp
• RefSeq ORF: 4629 bp
• Locus ID: 20563
• Cytogenetics: 5 B3
• MW: 171.7 kDa
• Gene Summary: The protein encoded by this gene is a member of the Slit family of secreted glycoproteins, which function as ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. In mammals, members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Mice deficient for this gene exhibit abnormal axonal projections in the embryonic forebrain and develop supernumerary uretic buds that maintain improper connections to the nephric duct. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]