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Ralgapb (NM_001291137) Mouse Tagged ORF Clone
CAT#: MR231825
- TrueORF®
Ralgapb (myc-DDK-tagged) - Mouse Ral GTPase activating protein, beta subunit (non-catalytic) (Ralgapb), transcript variant 1
"NM_001291137" in other vectors (1)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Ralgapb |
| Synonyms | AI507211; B230339M05Rik; p170 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR231825 representing NM_001291137
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACTCTGAGTGGCGGTCACTGCATTTGGTGATTCAGAGTGATCAAGGCCATACCAGTGTGCTACACA GCTATCCAGAGAGTGTTGGGCGAGAGGTGGCGAATGCAGTGGTCCGTCCTCTTGGGCAGGCATTAGGTAC TTCTTCAGTGGCTGGTAGTGAGAGTTTATTAAAAACTGACAAAGAAGTAAAATGGACCATGGAAGTAATT TGCTATGGACTGACCCTTCCATTAGATGGAGAGACTGTAAAATATTGTGTTGATGTATATACAGATTGGA TTATGGCTTTGGTGTTGCCAAAAGATTCTATTCCATTACCCATTATTAAAGAACCTAATCTATATGTTCA AAGTATATTAAAACATTTACAAAATCTTTTTGTACCAAGACAGGAACAGGGCTCCAGTCAAATCCGACTA TGCTTACAAGTTCTGAGAGCCATTCAGAAACTGGCCCGGGAGTCATCCATCATGGCTCGAGAAACCTGGG AAGTCTTACTGTTGTTTCTTCTGCAAATTAATGACATACTTCTGGCCCCACCAACTGTTCAAGGTGGCAT TGCTGAGAATCTAGCAGAGAAGCTGATTGGTGTTCTCTTTGAAGTTTGGTTACTAGCTTGCACCCGGTGC TTCCCAACACCTCCTTATTGGAAGACAGCCAAGGAGATGGTGGCTAACTGGAGGCACCACCCTGCAGTGG TGGAGCAGTGGAGCAAGGTTATCTGTGCACTGACCTCCAGATTGCTCCGCTTTACATACGGTCCTTCATT TCCTCCATTTAAAGTTCCTGATGAAGATGCCAATCTCATCCCTCCAGAAATGGACAATGAGTGTATTGCA CAAACATGGTTTCGATTTTTACACATGCTAAGTAACCCCGTGGATCTGAGTAACCCAGCTGTTATAAGCT CAACTCCCAAGTTTCAAGAACAGTTCCTGAATGTGAGCGGGATGCCACAGGAGCTGAGCCAGTACCCCTG CCTTAAACATCTGCCTCAGATATTCTTTCGTGCCATGCGTGGAATCAGCTGTCTGGTGGATGCATTCTTA GGCATTTCTCGACCTCGATCAGACAGTGCTCCCCCAACACCCGTGAATAGATTAAGTATGCCCCAGAGTG CTGCTGTGAATACCACACCACCACATAACCGGAGGCACCGGGCTGTTACTGTGAACAAGGCCACAATGAA GACGAGTACAGTTACTACTGCTCACACCTCAAAAGTCCAGCACCAGGCATCCTCCACCTCTCCTCTGTCC AGTCCAAATCAGACTAGTTCAGAACCCAGGCCGCTGCCTGCCCCCCGAAGACCAAAGGTTAACAGCATCT TGAATCTCTTTGGATCGTGGTTATTCGATGCAGCATTTGTTCACTGTAAACTCCATAATGGAATAAACAG AGACAACAGCATGACTGCATCTTTTATCCAAATTCTTCTTTCTTATAAATCTTCCATTGCAACCCAAGCT AGCATGGAGTTTCGACGGAAAGGGTCCCAAATGTCAACAGACACCATGGTTTCCAACCCTGTGTTTGATG CCAGTGAATTTCCTGATAACTATGAAGCAGGAAGAGCTGAGGCTTGTGGGACATTATGTAGGATTTTTTG TAGCAAGAAGACTGGAGAAGAGATTCTGCCAGCTTATTTATCCAGATTTTACATGCTTTTAATTCAAGGT TTGCAGATAAATGATTATGTATGCCATCCTGTCTTGGCCAGCGTTATTCTAAACTCCCCTCCTTTGTTCT GCTGTGACTTGAAAGGGATTGATGTTGTGGTTCCTTACTTTATTTCAGCTCTTGAAACCATTTTGCCTGA CAGAGAACTCTCAAAATTCAAAAGCTATGTAAATCCAACAGAATTGAGAAGGTCATCCATTAATATCCTG CTTTCTTTGTTGCCCCTCCCTCATCATTTTGGCACAGTCAGATCTGAGGTGGTCCTAGAAGGAAAGTTTA GTAATGATGACAGCTCTTCTTATGATAAACCAATAACTTTTCTGTCCCTGAAGCTGAGACTTGTGAATAT ACTAATAGGTGCCTTGCAAACAGAAACGGACCCTAACAACACCCAAATGATACTAGGGGCAATGTTAAAT ATTGTTCAAGATTCTGCACTTTTAGAAGCCATTGGTTGCCAAATGGAAATGGGTGGTGGAGAAAACAACC TGAAGAGTCATAGTCGCACAAACAGTGGCATTAGCTCAGCAAGTGGAGGAAGCACAGAGCCTACAACCCC TGATAGCGAGAGACCTGCTCAAGCTCTTCTGAGGGATTATGCTCTTAATACAGATTCAGCTGCTGGGCTC CTGATTCGCAGCATTCATCTCGTCACTCAACGACTCAACTCCCAGTGGCGGCAAGATATGAGCATATCCT TGGCAGCTCTAGAGCTCCTCTCTGGCCTGGCAAAGGTGAAGGTGATGGTTGACTCAGGAGACCGGAAGCG TGCCATCAGTTCTGTGTGCAGCTACATCGTATACCAATGCAGTCGGCCAGCTCCTCTTCACTCCCGGGAT CTGCACTCCATGATAGTGGCAGCATTTCAGTGTCTCTGTGTCTGGCTGACAGAGCACCCTGATATGCTGG ATGAAAAGGATTGCCTTAAGGAAGTACTGGAGATTGTAGAACTGGGTATCTCAGGAAGCAAATCCAAGAA CAGTGAGCAAGAAGTCAAGTACAAAGGTGATAAGGAGCCGAACCCTGCATCCATGAGGGTAAAGGACGCC GCCGAAGCCACTCTAACATGTATCATGCAGTTGCTTGGCGCATTTCCTTCACCCAGTGGTCCTGCTTCTC CTTGTAGTCTGGTGAATGAGACCACTCTGATCAAATACTCCAGGCTGCCAACCATAAACAAGCATAGTTT CCGGTACTTTGTTTTGGATAACAGTGTCATCCTGGCAATGCTGGAGCAACCGCTTGGAAATGAGCAGAAT GACTTTTTCCCCTCTGTCACTGTGCTGGTCCGGGGAATGTCTGGAAGACTTGCGTGGGCTCAGCAGCTTT GCCTTTTACCCAGAGGAGCAAAAGCAAATCAGAAGCTTTTTGTGCCTGAACCTCGCCCAGTTCCTAAAAA TGATGTTGGATTTAAATATTCTGTGAAACATCGGCCATTTCCTGAAGAGGTGGACAAGATTCCTTTTGTA AAAGCAGACTTGAGCATTCCAGATTTACATGAAATTGTCACTGAAGAATTAGAAGAGAGGCATGAAAAAT TAAGAAGTGGTATGGCACAACAAATTGCTTATGAGATGCACCTGGAACAGCAGAGCGAGGGGGAACTGCA GAAGCGAAGCTTCCCTGACCCTGTTACGGACTGTAAGCCTCCACCTCCTGCCCAGGAATTTCAGACAGCC CGCCTTTTCCTTTCTCACTTTGGATTTTTATCCTTAGAGGCATTAAAGGAACCTGCAAATAGTCGTCTAC CTCCTCATCTTATTGCACTCGATTCTACCATACCTGGATTTTTTGATGACATTGGGTATCTGGATCTCCT GCCATGCCGGCCTTTTGACACTGTTTTTATTTTCTACATGAAGCCAGGTCAGAAAACAAACCAAGAGATT TTAAAGAATGTGGAGTCTTCCAGAAATGTTCAGCCACATTTCCTAGAATTTTTGCTCTCCCTGGGCTGGT CAGTAGATGTGGGGAAACACCCCGGTTGGACAGGACATGTGTCCACCAGTTGGTCAATCAATAGTTGTGA TGATGGTGAAGGGTCTGAACCAGATGAAATAACTTCCTCTGAAGACGTTGGGGCTAGCATTTTCAACGGG CAGAAGAAGGTGCTGTATTATGCTGATGCCCTAACAGAAATAGCTTTTGTGGTTCCTTCTCCTGTGGAGT CACTGACTGATTCATTGGAAAGTAACATCTCAGACCAAGATAGTGACTCAAATATGGATCTTATGCCGGG AATTCTGAAGCAGCCACCCCTGACACTTGAGTTGGTCCCCAATCACACAGACAGTCTTAATTCCTCACAG AGGCTCAGTCCCAGTTCCAGAATGAAGAAGCTGCCTCAGGGTCGCCCTGTGCCCCCGCTTGGACCTGAGA CAAGAGTATCTGTAGTCTGGGTGGAACGCTACGATGATATAGAAAACTTTCCACTCTCAGATCTGATGAC AGAGATCAGCACTGGTGTAGAGACTACTGCAAATAGTAGCACTTCTCTGAGATCTACAACTCTTGAAAAA GAAGTCCCAGTAATCTTCATTCACCCTTTAAACACTGGATTGTTCCGGATAAAAATCCAAGGAGCCACTG GAAAATTTAACATGGTCATACCTCTGGTTGATGGGATGATAGTCAGCCGTCGAGCACTTGGCTTTCTGGT GAGGCAGACGGTAATAAACATTTGTAGAAGAAAGAGACTGGAGAGTGATTCCTACAGTCCACCACATGTC CGTCGGAAACAGAAAATCACTGACATTGTCAACAAATATCGGAATAAGCAGTTGGAGCCAGAGTTTTATA CTGCCCTTTTCCAGGAGGTTGGACTCAAGAACTGCAGTTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231825 representing NM_001291137
Red=Cloning site Green=Tags(s) MYSEWRSLHLVIQSDQGHTSVLHSYPESVGREVANAVVRPLGQALGTSSVAGSESLLKTDKEVKWTMEVI CYGLTLPLDGETVKYCVDVYTDWIMALVLPKDSIPLPIIKEPNLYVQSILKHLQNLFVPRQEQGSSQIRL CLQVLRAIQKLARESSIMARETWEVLLLFLLQINDILLAPPTVQGGIAENLAEKLIGVLFEVWLLACTRC FPTPPYWKTAKEMVANWRHHPAVVEQWSKVICALTSRLLRFTYGPSFPPFKVPDEDANLIPPEMDNECIA QTWFRFLHMLSNPVDLSNPAVISSTPKFQEQFLNVSGMPQELSQYPCLKHLPQIFFRAMRGISCLVDAFL GISRPRSDSAPPTPVNRLSMPQSAAVNTTPPHNRRHRAVTVNKATMKTSTVTTAHTSKVQHQASSTSPLS SPNQTSSEPRPLPAPRRPKVNSILNLFGSWLFDAAFVHCKLHNGINRDNSMTASFIQILLSYKSSIATQA SMEFRRKGSQMSTDTMVSNPVFDASEFPDNYEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMLLIQG LQINDYVCHPVLASVILNSPPLFCCDLKGIDVVVPYFISALETILPDRELSKFKSYVNPTELRRSSINIL LSLLPLPHHFGTVRSEVVLEGKFSNDDSSSYDKPITFLSLKLRLVNILIGALQTETDPNNTQMILGAMLN IVQDSALLEAIGCQMEMGGGENNLKSHSRTNSGISSASGGSTEPTTPDSERPAQALLRDYALNTDSAAGL LIRSIHLVTQRLNSQWRQDMSISLAALELLSGLAKVKVMVDSGDRKRAISSVCSYIVYQCSRPAPLHSRD LHSMIVAAFQCLCVWLTEHPDMLDEKDCLKEVLEIVELGISGSKSKNSEQEVKYKGDKEPNPASMRVKDA AEATLTCIMQLLGAFPSPSGPASPCSLVNETTLIKYSRLPTINKHSFRYFVLDNSVILAMLEQPLGNEQN DFFPSVTVLVRGMSGRLAWAQQLCLLPRGAKANQKLFVPEPRPVPKNDVGFKYSVKHRPFPEEVDKIPFV KADLSIPDLHEIVTEELEERHEKLRSGMAQQIAYEMHLEQQSEGELQKRSFPDPVTDCKPPPPAQEFQTA RLFLSHFGFLSLEALKEPANSRLPPHLIALDSTIPGFFDDIGYLDLLPCRPFDTVFIFYMKPGQKTNQEI LKNVESSRNVQPHFLEFLLSLGWSVDVGKHPGWTGHVSTSWSINSCDDGEGSEPDEITSSEDVGASIFNG QKKVLYYADALTEIAFVVPSPVESLTDSLESNISDQDSDSNMDLMPGILKQPPLTLELVPNHTDSLNSSQ RLSPSSRMKKLPQGRPVPPLGPETRVSVVWVERYDDIENFPLSDLMTEISTGVETTANSSTSLRSTTLEK EVPVIFIHPLNTGLFRIKIQGATGKFNMVIPLVDGMIVSRRALGFLVRQTVINICRRKRLESDSYSPPHV RRKQKITDIVNKYRNKQLEPEFYTALFQEVGLKNCSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001291137 |
| ORF Size | 4521 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001291137.1, NP_001278066.1 |
| RefSeq Size | 8426 bp |
| RefSeq ORF | 4524 bp |
| Locus ID | 228850 |
| Cytogenetics | 2 H1 |
| MW | 168.4 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC229614 | Ralgapb (untagged) - Mouse Ral GTPase activating protein, beta subunit (non-catalytic) (Ralgapb), transcript variant 1 |
USD 1,419.00 |
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