Palld (NM_001293772) Mouse Tagged ORF Clone

CAT#: MR231793

Palld (myc-DDK-tagged) - Mouse palladin, cytoskeletal associated protein (Palld), transcript variant 1

Product Images

Circular map for MR231793

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Palld
• Synonyms: 2410003B16Rik; 6030492A02
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231793 representing NM_001293772
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAGACTTCCTCCCACGACTCCTTCTATGACTCCTTATCAGACGTGCAGGAAGAGGGCAAAAGTG
  CTGACTTCTTTCCAGGCCTCTCTGCTTTCCTCAGCCAGGAAGAAATAAATAAAAGCCTTGACCTGGCACG
  CAGAGCTATAGACAGCTCTGAAACTGAAGATTTTGACTCTGAGAAGGAGATCTCGCAGATTTTCAGCAAG
  TCTCCCATAAGCCTCTGCGAAACTCCTTCCCATGAGGAGCCGAAATCAGGCAAGCAGACTTCCTCAGAAC
  GACCTCAGGATAGCAGGCGAGCACCTGTCCAGCCTCTAACAGGAGATCAAGCTGAAAGGATCACTTCCCC
  AGGTTCAAAGAGAAAACCCGGGGTATCACCCCTGCTGGCCAGCCCCAGCTACATCCGGAGCCTCCGCAAG
  GCTGAAAAACGAGGTGCAAAAAATCCCAATCCAAGTTCAAAGCCCAAAACTGCCCAGCAAAGCAAGGCTG
  GCCCCCAGAGCCAACTGTGCGACAAGGCGGCTAGTTTCATCGAGGAACTGACCTCCATATTTAGAGAAGC
  GGCAAAGCCAAGGAACAGAAGCCCAAATGGTGAGTCCTCATCACCAGACAGTGGGTATCTGTCTCCTAAA
  AATCAGCCATCAGCCCTGATGAGTGCCTCAGCCAGTCAGAGCCCCACTGCAGACCAGCTAGATCAACTGG
  AGATGGACGCAGAAGTCAAGCAAGCCCAGGGCAGCCTTTGCTACCAGGCCCACCAGGCCTCGGAAGAGAC
  CTTGCCACTCGCTCACATCCCGCACCCACAGCCCCAGAAAGCCCGCCACTTGCCAACTGCACCTAGGTTC
  ATCCAGAAACTGAGGAGCCAAGAAGTTGCTGAAGGAAGCAGAGTTTATTTGGAGTGTAGAGTCACAGGAA
  ACCCAACGCCCAGGGTCAGATGGTTCTGTGAGGGGAAGGAGCTGTACAACAGTCCTGACGTTCAGATCCA
  CTGCGAGAGTGGGGAGCTGCACACCTTGGTCATTGCTGAGGCCTTTGAGGACGACACAGGTCGCTACACG
  TGTCTGGCTACAAACCCCAGTGGCTCAGACAGCACATCTGCAGAGGTGTTCATTGAAGGAGCCAGTTCAA
  CGGACTCTGACAGCGAAAGCTTATCTTTCATATCAAAAGCTGGAGCCATGCCACAAGCTCAGAAGAAAAC
  AACGTCCGTTTCCTTAACGATAGGATCCTCAGCTCCGAAAACAGGAGTGACCACAGCTGTGATTCAGCCC
  CTATCTGTGCCCGTCCAACAGGCTCACAGCGCTACTTCATATCTCTGCCGACCTGACGGAACCACCATGG
  GCTGCCTTCTTCCTGTTTTTACTAAGGAACTACAGAACACAGCAGCCTCCGAGGGCCAGGTGGTGGTGCT
  GGAATGCAGAGTCCGAGGGGCACCCCCGCTGCAGGTCCAGTGGTTCCGGCAAGGAAGTGAGATCCAAGAC
  TCTCCGGACTTCAGAATTCTCCAGAAAAAACCCAGATCGACAGCTGAACCCGAGGAGATCTGCACCCTTG
  TCATTGCTGAGAGTTTCCCTGAAGATGCAGGCATCTTTACCTGCTCAGCCACAAATGACTACGGCTCAGT
  GACCAGCACTGCACAGCTTGTTATAACCTCCGCCAACAACGAGAACTGTAGTTACGACTCAACAGGAGAA
  CCCAACAGTGATCACTTCCAACACTTTCCACCTCCCCCTCCGATCCTGGAGACAGGCTCCTATGAGCTGG
  CATCCCAGAAACCATCTGAAATCCAGCAGGTGAACAGCCCCAATTTAGGATTTAGCATGGCAGCTCTTCA
  AATGCAATTCAACACTGCAGAGAGGGAGACCAACGGAGTCCATCCCAGCCATGGAGTCAACGGGCTGATT
  AATGGCAAAGCTTATGGCAATAAATCTCCTCCAACACCAACTGCCCTGCTTTCACCCACTAAGGAACCAC
  CGCCACTCCTTGCCAAACCCAAACTGGATCCTCTGAAGCTTCAGCAGCTGCAGAACCAAGTGCGCCTGGA
  ACAGGAGGCCTGCGCCTGGCCCCCAGCGCCCCCAGGTGTCCCTTGCAACAGCAGCAGCAGCGGCAGCAGC
  GCGCCCCCGTCGCCGCCCTTCCCCCCACCGCCACCAGCCTTTCCGGAGCTCGCGGCCTGTGCGTCGCCGG
  TGCCCTCGGAGCCCATGAGCGCTCTGGCCTCCCGCGCTACCGCCATGCAGTCCTCCGGCTCCTTCAACTA
  CGCGCGCCCTAAGCAGTTCATCGCGGCGCAGAACCTGGGTCCCGCATCGGGGCTGCCCACGCCAACCTCC
  AGCCCCAGCTCCTCCAGCTTGCCGTCACCATTGTCCCCCACGCCCAGGCCCTTCGGCCGCGCGCCCGGGC
  CGCCCTTCGTGGAACCCGAGGCCATGTGGGGCCCGTCCTCGCCCTCGCCGCCACCGCCGCCACCTCCGGT
  CTTCAGCCCCTCTGCAGCCTACCCGGTGCCCGACGTGTTCCCACTGCCACCGCCGCCGCCACCGCTACCT
  AGCTCCACCTCACACTGCGCCTCGCCCGCCCGCTTCGGCCCCAGCCAGACGCCCGCAGCCTTCCTCAGCG
  CCCTGCTGCCCTCTCAGCCACCGCCTGTCGCTGTCAACGCCCTGGGGCTGCCCAAGGGAGTCACCCCCGC
  GGGGTTCCCAAAGAAGTCCAGTAGAACTGCTAGAATTGCCTCTGATGAGGAGATTCAAGGCACAAAGGAT
  GCTGTCATCCAAGACCTGGAACGGAAGCTTCGCTTCAAGGAGGACCTTCTGAACAATGGCCAACCGAGGC
  TAACCTATGAGGAAAGAATGGCTCGCCGCCTGCTTGGAGCCGACAGCGCAAACGTCTTCAACATCCAGGA
  GCCAGAGGAAACGGCAGCCAATCAGGACGCTGGGGCTCCTCGGGCTTCTGTAGGGGGTCCTCTGGATGGT
  CAAAAGGAGTACAAAGTCTCTAGCTGCGAGCAGAGGCTGATTAGCGAGATTGAGTACCGGCTGGAGCGCT
  CCCCTGTGGATGAGTCGGGAGACGAGGTGCAGGATCCAGATGTGCCTGTGGAGAACGCAACAGCTCCCTT
  CTTTGAGATGAAGCTGAAACACTACAAGATCTTTGAGGGGATGCCGGTGACTTTCACGTGTCGAGTGGCT
  GGGAATCCAAAGCCAAAGATCTATTGGTTTAAAGATGGGAAGCAGATTTCTCCGAAGAGCGATCACTACA
  CCATTCAGAGAGACCTTGATGGGACCTGCTCTCTCCACACCACGGCCTCTACCCTAGACGACGATGGGAA
  CTACACCATCATGGCTGCCAACCCTCAGGGTCGCGTCAGTTGTACAGGAAGGCTAATGGTACAGGCTGTC
  AACCAAAGAGGCCGCAGTCCCCGCTCTCCCTCAGGCCATCCTCATGCCAGAAGGCCTCGCTCTCGATCAC
  GGGACAGTGGAGATGAAAACGAGCCCATTCAGGAGCGATTCTTCAGACCTCACTTCCTGCAGGCTCCTGG
  AGACCTGACCGTTCAGGAAGGCAAGCTCTGCAGGATGGACTGCAAGGTCAGTGGGTTACCAACCCCAGAT
  CTCAGCTGGCAACTAGATGGAAAGCCCATACGCCCCGACAGTGCTCACAAGATGCTGGTCCGTGAGAATG
  GGGTCCACTCCCTCATTATAGAGCCAGTCACGTCCCGGGACGCCGGCATCTACACATGTATTGCCACCAA
  CAGAGCAGGACAGAACTCGTTTAACCTGGAGCTTGTGGTTGCTGCTAAGGAAGCACACAAGGCCCCTGTG
  TTTATGGAGAAGCTACAGAACACGGGGGTTGCTGATGGATACCCAGTGCGGCTGGAATGCCGTGTCTCGG
  GAGTGCCGCCACCTCAGATATTTTGGAAGAAAGAAAATGAATCGCTCACTCACAGCACTGAGCGAGTAAG
  CATGCACCAGGATAATCATGGCTACATCTGCCTGCTCATCCAGGGAGCCACAAAGGAAGACGCTGGGTGG
  TACACTGTGTCCGCCAAGAACGAAGCAGGCATTGTGTCCTGCACTGCCCGGCTGGATGTCTACACCCAGT
  GGCACCAGCAGCCACAGACCACCAAGCCAAAAAAAGTACGGCCCTCGGCCAGTCGCTACGCAGCACTTTC
  GGACCAGGGACTAGACATCAAAGCCGCTTTCCAACCTGAAGCCAGCCCATCTCACCTGACTCTGAACAGC
  GGCTTGGTAGAAAGTGAAGACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231793 representing NM_001293772
  Red=Cloning site Green=Tags(s)
  
  MSETSSHDSFYDSLSDVQEEGKSADFFPGLSAFLSQEEINKSLDLARRAIDSSETEDFDSEKEISQIFSK
  SPISLCETPSHEEPKSGKQTSSERPQDSRRAPVQPLTGDQAERITSPGSKRKPGVSPLLASPSYIRSLRK
  AEKRGAKNPNPSSKPKTAQQSKAGPQSQLCDKAASFIEELTSIFREAAKPRNRSPNGESSSPDSGYLSPK
  NQPSALMSASASQSPTADQLDQLEMDAEVKQAQGSLCYQAHQASEETLPLAHIPHPQPQKARHLPTAPRF
  IQKLRSQEVAEGSRVYLECRVTGNPTPRVRWFCEGKELYNSPDVQIHCESGELHTLVIAEAFEDDTGRYT
  CLATNPSGSDSTSAEVFIEGASSTDSDSESLSFISKAGAMPQAQKKTTSVSLTIGSSAPKTGVTTAVIQP
  LSVPVQQAHSATSYLCRPDGTTMGCLLPVFTKELQNTAASEGQVVVLECRVRGAPPLQVQWFRQGSEIQD
  SPDFRILQKKPRSTAEPEEICTLVIAESFPEDAGIFTCSATNDYGSVTSTAQLVITSANNENCSYDSTGE
  PNSDHFQHFPPPPPILETGSYELASQKPSEIQQVNSPNLGFSMAALQMQFNTAERETNGVHPSHGVNGLI
  NGKAYGNKSPPTPTALLSPTKEPPPLLAKPKLDPLKLQQLQNQVRLEQEACAWPPAPPGVPCNSSSSGSS
  APPSPPFPPPPPAFPELAACASPVPSEPMSALASRATAMQSSGSFNYARPKQFIAAQNLGPASGLPTPTS
  SPSSSSLPSPLSPTPRPFGRAPGPPFVEPEAMWGPSSPSPPPPPPPVFSPSAAYPVPDVFPLPPPPPPLP
  SSTSHCASPARFGPSQTPAAFLSALLPSQPPPVAVNALGLPKGVTPAGFPKKSSRTARIASDEEIQGTKD
  AVIQDLERKLRFKEDLLNNGQPRLTYEERMARRLLGADSANVFNIQEPEETAANQDAGAPRASVGGPLDG
  QKEYKVSSCEQRLISEIEYRLERSPVDESGDEVQDPDVPVENATAPFFEMKLKHYKIFEGMPVTFTCRVA
  GNPKPKIYWFKDGKQISPKSDHYTIQRDLDGTCSLHTTASTLDDDGNYTIMAANPQGRVSCTGRLMVQAV
  NQRGRSPRSPSGHPHARRPRSRSRDSGDENEPIQERFFRPHFLQAPGDLTVQEGKLCRMDCKVSGLPTPD
  LSWQLDGKPIRPDSAHKMLVRENGVHSLIIEPVTSRDAGIYTCIATNRAGQNSFNLELVVAAKEAHKAPV
  FMEKLQNTGVADGYPVRLECRVSGVPPPQIFWKKENESLTHSTERVSMHQDNHGYICLLIQGATKEDAGW
  YTVSAKNEAGIVSCTARLDVYTQWHQQPQTTKPKKVRPSASRYAALSDQGLDIKAAFQPEASPSHLTLNS
  GLVESEDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001293772
• ORF Size: 4224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001293772.2
• RefSeq Size: 6378 bp
• RefSeq ORF: 4227 bp
• Locus ID: 72333
• UniProt ID: Q9ET54
• Cytogenetics: 8 B3.1
• MW: 152.6 kDa
• Gene Summary: Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific May be required for the initiation of neural tube closure.[UniProtKB/Swiss-Prot Function]