Smc2 (NM_001301412) Mouse Tagged ORF Clone

CAT#: MR231669

Smc2 (myc-DDK-tagged) - Mouse structural maintenance of chromosomes 2 (Smc2), transcript variant 1

Product Images

Circular map for MR231669

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Smc2
• Synonyms: 5730502P04Rik; AI255214; AW545314; CAP; CAP-E; CAPE; Fin; Fin16; SMC-2; Smc2l1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231669 representing NM_001301412
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATGTTAAATCAATCATTCTCGAAGGATTCAAGTCCTATGCACAGAGGACTGAAGTCAATGGTTTTG
  ACCCCCTCTTCAATGCGATCACTGGTTTAAATGGTAGTGGAAAATCCAACATATTGGACTCCATATGCTT
  TCTCCTAGGCATCTCCAACCTGTCCCAGGTGCGGGCTTCAAATTTACAAGATCTCGTTTATAAAAATGGA
  CAGGCTGGGATTACCAAAGCCTCTGTTTCAATCACTTTTGATAATTCTGACAAAAAGCAGAGTCCGTTGG
  GATTTGAAGCTCATGATGAAATCACAGTAACAAGACAGGTGGTTATTGGTGGCAGAAATAAATACTTAAT
  CAATGGAGTGAATGCCAACAACACCAGAGTCCAAGATCTCTTTTGTTCTGTGGGCCTGAATGTTAACAAC
  CCTCACTTTCTCATCATGCAGGGCAGAATTACGAAAGTATTAAATATGAAACCACCAGAGATATTATCCA
  TGATTGAAGAAGCTGCTGGAACCAGGATGTATGAGTACAAAAAAATAGCCGCCCAGAAAACTATAGAAAA
  AAAGGAGGCTAAGCTGAAAGAAATAAAAACGATACTGGAAGAAGAGATTACTCCAACGATTCAAAAATTA
  AAAGAGGAAAGATCTTCTTACTTGGAGTACCAGAAAGTAATGCGGGAGATAGAGCATCTGAGTCGTTTAT
  ACATTGCCTATCAGTTTTTGCGGGCTGAAGACACCAAGGAACGCTCAGCTGGGGAGCTAAAGGAAATGCA
  GGATAAAATAGTAAATCTTCAAGAAGTACTGTCAGAGAATGAAAAAAAAATAAAGGCTCTCAATTGTGAA
  ATCGAAGAATTGGAGAGAAGAAAAGATAAGGAAACTGGAGGCAAACTCAAGTCTCTAGAGGATGCTTGTG
  CAGAAGCCCAGCGAGTTAATACTAAGTCTCAAAGTGCATTTGACCTCAAGAAGAAAAACCTAGCTAGTGA
  AGAAACCAAGCGCAAAGAGCTACAAAACAGTATGGCTGAGGATTCTAAAGCATTAGCAGCAAAGGAAAAA
  GAAGTTAAGAAGATAACAGATGGACTGCATGGCCTTCAAGAAGCAAGCAATAAAGATGCTGAAGCTTTGG
  CTGCTGCACAGCAGCACTTCAATGCTGTTTCTGCTGGCTTGTCCAGTAACGAAGATGGAGCAGAAGCAAC
  TCTGGCTGGGCAGATGATAGCCTGTAAAAACGATATCAGCAAAGCTCAGACTGAGGCCAAGCAGGCTCAG
  ATGAAGCTGAAACATGCCCAACAAGAACTAAAGAGTAAGCAAGCAGAGGTTAAGAAGATGGACAGTGGCT
  ACAAGAAGGATCAAGACGCTTTTGAAGCTGTGAAAAAAGCTAAAGAAAAACTTGAAACTGAAATGAAGAA
  GCTAAATTACGAAGAAAACAAAGAGGAAAAGCTTTTGGAAAAGCACAGGCAGCTGTCCCGTGACATCAAC
  AACCTAAAAGGAAAACATGAAGCTCTCTTGGCCAAGTTTCCCAATCTTCAATTTGCATACAAGGACCCAG
  AGAAGAACTGGAATCGAAATTCTGTGAAAGGACTTGTAGCTTCTCTGATAAATGTGAAAGATAATTCTAC
  AGCCACAGCTCTGGAAGTGGTGGCTGGGGAACGACTCTACAATGTTGTAGTAGACACAGAGGTAACTGCT
  AAGAAGCTACTAGAAAAGGGGGAGTTGAAGCGTCGATACACAATAATTCCTCTCAATAAGATTTCAGCCA
  GATGTATTGCTCCTGAAACTCTGAGGGTCGCTCAGAATCTTGTTGGTCCTGACAATGTTCATGTAGCTCT
  TTCCTTGGTTGACTATAAACCAGAACTTCAGAAAGGAATGGAATTTGTCTTTGGAACAACATTTGTTTGT
  AACAATATGGATAATGCCAAAAAAGTGGCCTTTGATAAAAGGATAATGACCAGAACTGTAACCCTTGGGG
  GTGATGTATTTGATCCTCATGGAACATTGAGTGGAGGTGCTAGGTCCCAGGCAGCTTCGATTTTAACCAA
  GTTTCAAGAAGTCAAAGATGTTCAAGATGAGCTGAGGACCAAGGAGAATGAACTGCGCGCTCTAGAAGAG
  GAGTTAGCAGGTCTTAAAAATGTTGCTGAAAAGTATCGCCAACTGAAACAGCAGTGGGAGATGAAGACAG
  AGGAGGGAGACTTATTGCAAACTAAACTTCAGCAAAGCTCTTATCATAAGCAACAAGAAGAATTAGATGC
  CCTTAAAAAAACCATTGAGGAAAGTGAGGAGACATTAAAAAGCACAAAAGAAATCCAAAAGAAAGCTGAA
  GAAAAGTATGAAGCATTGGAGAATAAAATGAAAAATGCGGAAGCTGAAAGAGAGAAAGAACTGAAGGATG
  CTCAGAAAAAACTAGATTGTGCCAAAACAAAGGCAGATGCATCTAGCAAGAAAATGAAAGAAAAACAACA
  GGAGGTTGAAGCTATTACTCTAGAGCTGGAGGAGCTCAAAAGAGAGCATGCTTCTAATGAGCAGCAGCTT
  GATGCCGTGAATGAAGCCATCAAAGCCTATGAAGGTCAGATTGAAAAAATGGCAGCTGAGGTCGCTAAAA
  ATAAGGAATCAGTAAATAAAGCTCAAGACGAGCTGATGAAGCAAAAGCAAATAATAACAGCCCAGGACAA
  CATAATTAAAGATAAATGTGCAGAAGTGGCGAAACACAATTTGCAAAACAATGAGTCTCAACTTAAAATT
  AAGGAGTTGGACCACAGCATAAGCAAACATAAGCGGGAAGCGGATGATGCTGCTGCAAAGGTATCCAAAA
  TGTTAAGTGATTATGATTGGATTAATGCTGAGAAACACCTATTTGGCCAACCTAATAGTGCCTATGATTT
  CAAAACAAACAACCCCAAAGAAGCTGGCCAGAGACTTCAGAAGTTACAGGAAGTAAAAGAGAAACTAGGG
  AGAAATGTCAACCTGAGAGCTATGAACGTGCTGACAGAAGCTGAAGAGCGGTATAATGATTTGATGAAGA
  AGAAAAGAATTGTAGAAAATGACAAATCCAAAATCCTTGCAACTATAGAAGATCTTGACCAGAAGAAAAA
  CCAGGCCCTAAATATTGCTTGGCAAAAGGTGAACAAAGACTTTGGCTCTATTTTTTCTACCCTTTTGCCT
  GGTGCCAATGCTATGCTTGCACCACCAGAAGGGCAGACAGTTTTAGATGGTCTGGAGTTCAAGGTTGCCT
  TAGGAAATACGTGGAAAGAAAACCTAACTGAGCTTAGTGGTGGTCAGAGGTCTCTAGTGGCTCTCTCACT
  AATATTGTCCATGCTCCTCTTCAAACCTGCTCCAATTTATATCTTGGATGAGGTGGATGCGGCCTTGGAT
  CTTTCTCATACCCAGAATATTGGGCAGATGCTGCGGACTCACTTCACACATTCTCAATTCATTGTGGTAT
  CCCTCAAAGAAGGTATGTTCAACAATGCAAACGTTCTCTTCAAAACCAAGTTTGTGGATGGTGTTTCTAC
  AGTAGCCAGATTTACGCAGAGTCAAGCTGGGAAGATTCCGAAGGAAGCAAAGTCCAGAGGAAAAGAACCC
  AAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231669 representing NM_001301412
  Red=Cloning site Green=Tags(s)
  
  MYVKSIILEGFKSYAQRTEVNGFDPLFNAITGLNGSGKSNILDSICFLLGISNLSQVRASNLQDLVYKNG
  QAGITKASVSITFDNSDKKQSPLGFEAHDEITVTRQVVIGGRNKYLINGVNANNTRVQDLFCSVGLNVNN
  PHFLIMQGRITKVLNMKPPEILSMIEEAAGTRMYEYKKIAAQKTIEKKEAKLKEIKTILEEEITPTIQKL
  KEERSSYLEYQKVMREIEHLSRLYIAYQFLRAEDTKERSAGELKEMQDKIVNLQEVLSENEKKIKALNCE
  IEELERRKDKETGGKLKSLEDACAEAQRVNTKSQSAFDLKKKNLASEETKRKELQNSMAEDSKALAAKEK
  EVKKITDGLHGLQEASNKDAEALAAAQQHFNAVSAGLSSNEDGAEATLAGQMIACKNDISKAQTEAKQAQ
  MKLKHAQQELKSKQAEVKKMDSGYKKDQDAFEAVKKAKEKLETEMKKLNYEENKEEKLLEKHRQLSRDIN
  NLKGKHEALLAKFPNLQFAYKDPEKNWNRNSVKGLVASLINVKDNSTATALEVVAGERLYNVVVDTEVTA
  KKLLEKGELKRRYTIIPLNKISARCIAPETLRVAQNLVGPDNVHVALSLVDYKPELQKGMEFVFGTTFVC
  NNMDNAKKVAFDKRIMTRTVTLGGDVFDPHGTLSGGARSQAASILTKFQEVKDVQDELRTKENELRALEE
  ELAGLKNVAEKYRQLKQQWEMKTEEGDLLQTKLQQSSYHKQQEELDALKKTIEESEETLKSTKEIQKKAE
  EKYEALENKMKNAEAEREKELKDAQKKLDCAKTKADASSKKMKEKQQEVEAITLELEELKREHASNEQQL
  DAVNEAIKAYEGQIEKMAAEVAKNKESVNKAQDELMKQKQIITAQDNIIKDKCAEVAKHNLQNNESQLKI
  KELDHSISKHKREADDAAAKVSKMLSDYDWINAEKHLFGQPNSAYDFKTNNPKEAGQRLQKLQEVKEKLG
  RNVNLRAMNVLTEAEERYNDLMKKKRIVENDKSKILATIEDLDQKKNQALNIAWQKVNKDFGSIFSTLLP
  GANAMLAPPEGQTVLDGLEFKVALGNTWKENLTELSGGQRSLVALSLILSMLLFKPAPIYILDEVDAALD
  LSHTQNIGQMLRTHFTHSQFIVVSLKEGMFNNANVLFKTKFVDGVSTVARFTQSQAGKIPKEAKSRGKEP
  N
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301412
• ORF Size: 3573 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301412.1, NP_001288341.1
• RefSeq Size: 5531 bp
• RefSeq ORF: 3576 bp
• Locus ID: 14211
• UniProt ID: Q8CG48
• Cytogenetics: 4 28.31 cM
• MW: 134.2 kDa
• Gene Summary: The protein encoded by this gene is a component of both condensin I and condensin II complexes, and forms a heterodimer with structural maintenance of chromosome 4 (Smc4). This heterodimer is the catalytic subunit for both condensin complexes, and is involved in several processes, including chromosome condensation during mitosis and meiosis, cohesin removal during mitosis and meiosis, and single-strand break (SSB) repair. Reduced expression of this gene results in chromosome segregation defects during mitosis and meiosis, with a more severe defect observed in embryonic stem cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]