Kcnma1 (NM_001253376) Mouse Tagged ORF Clone

CAT#: MR231661

Kcnma1 (myc-DDK-tagged) - Mouse potassium large conductance calcium-activated channel, subfamily M, alpha member 1 (Kcnma1), transcript variant 20

Product Images

Circular map for MR231661

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Kcnma1
• Synonyms: 5730414M22Rik; BKCa; MaxiK; mSlo; mSlo1; Slo; Slo1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231661 representing NM_001253376
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAACGGTGGCGGCGGCGGCGGCGGCAGCAGCGGCGGCGGCGGCGGCGGCGGCGGAGGCAGCGGTC
  TTAGAATGAGCAGCAATATCCACGCGAACCATCTCAGCCTAGACGCGTCCTCCTCCTCCTCTTCCTCCTC
  CTCTTCTTCTTCTTCCTCCTCCTCTTCCTCCTCGTCCTCGGTCCACGAGCCCAAGATGGATGCGCTCATC
  ATACCGGTGACCATGGAGGTGCCGTGCGACAGCCGGGGCCAACGCATGTGGTGGGCTTTCTTGGCCTCCT
  CCATGGTGACTTTCTTCGGGGGCCTCTTCATCATCTTGCTCTGGCGGACGCTCAAGTACCTGTGGACCGT
  TTGCTGCCACTGCGGGGGCAAGACGAAGGAGGCCCAGAAGATAAACAATGGCTCCAGCCAGGCAGATGGT
  ACTCTCAAGCCAGTGGACGAAAAAGAGGAGGTGGTGGCAGCCGAGGTCGGCTGGATGACATCTGTGAAGG
  ACTGGGCAGGGGTGATGATATCCGCCCAGACACTGACTGGCAGAGTCCTGGTTGTGTTAGTCTTTGCTCT
  CAGCATTGGTGCCCTCGTAATATACTTCATAGACTCGTCAAACCCAATAGAATCCTGCCAGAATTTCTAC
  AAAGATTTCACATTACAGATCGACATGGCTTTCAACGTGTTCTTCCTCCTCTACTTTGGCTTGCGGTTTA
  TTGCAGCCAACGATAAGCTGTGGTTCTGGCTGGAAGTGAATTCAGTAGTAGATTTCTTCACAGTCCCTCC
  TGTGTTTGTGTCTGTGTACTTAAACAGAAGTTGGCTTGGCTTGAGATTTTTAAGAGCTCTCAGACTGATA
  CAGTTTTCAGAGATTTTGCAGTTTCTGAATATCCTTAAAACAAGTAACTCCATCAAGCTGGTGAATCTGC
  TCTCCATATTTATCAGCACGTGGCTGACTGCAGCTGGATTCATCCACTTGGTGGAGAATTCAGGGGACCC
  ATGGGAAAATTTCCAAAACAACCAGGCACTTACGTACTGGGAATGTGTCTACTTACTCATGGTCACAATG
  TCTACAGTGGGTTATGGGGACGTTTATGCAAAAACCACACTTGGACGCCTCTTCATGGTCTTCTTCATCC
  TCGGGGGACTGGCCATGTTTGCCAGCTACGTCCCTGAAATCATAGAGTTAATAGGAAACCGCAAGAAATA
  CGGGGGCTCCTATAGCGCGGTTAGTGGAAGAAAGCACATTGTAGTCTGTGGACACATTACTCTGGAGAGT
  GTCTCTAACTTCCTGAAGGACTTTCTGCACAAGGACCGGGATGATGTCAACGTGGAGATTGTCTTTCTTC
  ACAACATCTCCCCTAACCTTGAGCTTGAGGCTCTGTTCAAACGGCATTTCACTCAGGTGGAGTTTTATCA
  GGGCTCTGTCCTCAATCCACATGATCTTGCCAGAGTCAAGATAGAGTCAGCAGATGCATGCCTGATCCTT
  GCCAATAAGTATTGCGCTGACCCGGATGCAGAAGATGCCTCCAACATCATGAGAGTGATCTCCATCAAAA
  ACTACCACCCAAAGATCAGGATCATCACTCAGATGCTGCAGTATCACAACAAGGCCCATCTGCTCAACAT
  CCCCAGCTGGAACTGGAAAGAGGGTGATGACGCAATATGCCTTGCAGAGCTCAAGTTGGGTTTCATAGCC
  CAGAGCTGTCTGGCTCAAGGCCTCTCCACAATGCTTGCCAATCTCTTCTCTATGAGGTCATTCATAAAGA
  TTGAGGAAGACACATGGCAGAAATACTACTTGGAAGGAGTCTCCAATGAAATGTACACAGAATATCTCTC
  CAGTGCCTTCGTGGGTCTGTCCTTCCCTACTGTTTGTGAGCTGTGTTTTGTGAAGCTTAAGCTCCTGATG
  ATAGCCATTGAGTACAAGTCTGCCAACAGAGAGAGCCGAATATTAATTAACCCTGGGAACCACCTTAAGA
  TCCAAGAAGGTACTTTAGGATTTTTCATCGCAAGTGATGCCAAAGAAGTTAAAAGGGCATTTTTTTACTG
  CAAGGCCTGTCATGATGACGTCACAGATCCCAAAAGAATTAAAAAATGTGGCTGCAGGCGGCTTGAAGAT
  GAGCAGCCGCCAACCCTGTCACCAAAAAAAAAACAACGTAATGGGGGCATGAGGAACTCGCCCAACACCT
  CCCCGAAGCTGATGAGGCATGACCCCTTGTTAATTCCTGGCAATGATCAGATTGACAACATGGACTCCAA
  TGTGAAAAAGTACGACTCCACTGGAATGTTTCACTGGTGTGCACCCAAGGAGATTGAGAAAGTCATCTTG
  ACTCGAAGTGAAGCTGCCATGACTGTCCTGAGTGGCCATGTCGTAGTCTGCATCTTTGGGGATGTCAGCT
  CAGCCCTGATTGGCCTCCGGAACCTGGTGATGCCACTTCGTGCTAGCAACTTTCACTATCATGAGCTCAA
  ACACATTGTGTTTGTGGGCTCCATTGAGTACCTCAAGAGGGAGTGGGAAACACTGCACAACTTCCCGAAA
  GTGTCCATATTGCCTGGTACACCATTAAGTCGGGCTGATTTAAGGGCTGTCAACATCAACCTCTGTGACA
  TGTGCGTTATCCTGTCAGCCAATCAGAATAATATTGATGATACTTCGCTTCAGGACAAGGAATGCATCTT
  GGCGTCACTCAACATCAAATCTATGCAGTTTGATGACAGCATCGGGGTCTTGCAGGCTAATTCCCAAGGA
  TTCACACCTCCTGGAATGGACAGATCATCACCCGACAACAGCCCAGTGCACGGGATGTTACGCCAGCCGT
  CCATCACAACTGGGGTCAACATTCCCATCATCACGGAACTCGTGAATGATACCAATGTTCAGTTTTTGGA
  CCAAGACGATGACGATGACCCTGACACAGAGCTGTACCTCACACAGCCCTTTGCTTGTGGGACAGCATTT
  GCCGTCAGCGTCCTGGACTCACTCATGAGCGCGACATACTTCAATGACAATATCCTCACCCTAATACGGA
  CCCTGGTGACAGGAGGAGCCACACCAGAGCTCGAGGCTCTAATAGCTGAGGAGAATGCACTTCGAGGAGG
  CTACAGCACTCCGCAGACATTGGCCAACAGGGACCGTTGCCGAGTGGCCCAGTTAGCCCTGTTAGATGGT
  CCCTTTGCAGACTTAGGGGATGGTGGTTGTTATGGTGATCTGTTCTGCAAAGCTCTGAAAACATATAATA
  TGCTTTGTTTTGGAATTTACCGGCTGAGAGATGCCCACCTCAGCACCCCCAGCCAGTGTACAAAAAGGTA
  CGTCATCACCAATCCTCCCTACGAGTTTGAGCTCGTACCAACAGACCTGATCTTCTGCCTGATGCAGTTT
  GACCACAACGCTGGCCAATCCCGGGCCAGTCTGTCTCATTCCTCCCACTCCTCACAGTCGTCCAGTAAGA
  AGAGCTCCTCCGTCCACTCCATCCCGTCCACAGCAAATCGGCCGAACCGGCCCAAGTCCAGGGAGTCCCG
  CGACAAACAGAAGTACGTTCAGGAAGAGCGGCTT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231661 representing NM_001253376
  Red=Cloning site Green=Tags(s)
  
  MANGGGGGGGSSGGGGGGGGGSGLRMSSNIHANHLSLDASSSSSSSSSSSSSSSSSSSSSVHEPKMDALI
  IPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGGKTKEAQKINNGSSQADG
  TLKPVDEKEEVVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFY
  KDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLI
  QFSEILQFLNILKTSNSIKLVNLLSIFISTWLTAAGFIHLVENSGDPWENFQNNQALTYWECVYLLMVTM
  STVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLES
  VSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLIL
  ANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIA
  QSCLAQGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVSNEMYTEYLSSAFVGLSFPTVCELCFVKLKLLM
  IAIEYKSANRESRILINPGNHLKIQEGTLGFFIASDAKEVKRAFFYCKACHDDVTDPKRIKKCGCRRLED
  EQPPTLSPKKKQRNGGMRNSPNTSPKLMRHDPLLIPGNDQIDNMDSNVKKYDSTGMFHWCAPKEIEKVIL
  TRSEAAMTVLSGHVVVCIFGDVSSALIGLRNLVMPLRASNFHYHELKHIVFVGSIEYLKREWETLHNFPK
  VSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDTSLQDKECILASLNIKSMQFDDSIGVLQANSQG
  FTPPGMDRSSPDNSPVHGMLRQPSITTGVNIPIITELVNDTNVQFLDQDDDDDPDTELYLTQPFACGTAF
  AVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALLDG
  PFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQF
  DHNAGQSRASLSHSSHSSQSSSKKSSSVHSIPSTANRPNRPKSRESRDKQKYVQEERL
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253376
• ORF Size: 3534 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253376.1, NP_001240305.1
• RefSeq Size: 6020 bp
• RefSeq ORF: 3537 bp
• Locus ID: 16531
• Cytogenetics: 14 A3
• MW: 131.4 kDa
• Gene Summary: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).[UniProtKB/Swiss-Prot Function]