Tsc1 (NM_001289575) Mouse Tagged ORF Clone

CAT#: MR231634

Tsc1 (myc-DDK-tagged) - Mouse tuberous sclerosis 1 (Tsc1), transcript variant 1

Product Images

Circular map for MR231634

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tsc1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231634 representing NM_001289575
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGTTAGCCAACATTGGGGAGCTGCTCTCCATGCTGGACTCCTCCACACTGGGTGTGCGGGATG
  ACGTGACAGCCATCTTCAAGGAGTCCCTCAATTCTGAACGTGGGCCTATGCTTGTCAACACGTTGGTTGA
  TTATTACCTGGAAACCAATTCTCAGCCGGTATTGCACATCCTGACCACCTTGCAGGAGCCACACGATAAG
  CACCTCTTGGACAAAATTAATGAGTATGTAGGCAAAGCTGCTACCCGTTTATCCATCCTCTCGCTGCTGG
  GGCATGTTGTGAGACTGCAGCCATCTTGGAAGCATAAGCTCTCTCAAGCACCTCTTCTGCCTTCTTTATT
  GAAATGTCTCAAGATGGACACTGATGTTGTGGTCCTCACAACTGGTGTCTTGGTGTTGATCACCATGCTC
  CCGATGATCCCGCAGTCAGGGAAGCAGCACCTTCTCGACTTCTTTGACATCTTTGGCCGTCTCTCGTCAT
  GGTGCCTGAAGAAACCAGGCCATGTGACAGAAGTGTACCTGGTCCATCTTCATGCCAGTGTTTATGCCCT
  CTTTCACCGCCTTTATGGGATGTACCCATGTAACTTCGTCTCCTTCCTGCGCTCTCACTACAGTATGAAG
  GAAAACGTGGAGACTTTTGAAGAAGTGGTCAAGCCAATGATGGAGCATGTGCGAATTCACCCGGAATTAG
  TGACTGGATCCAAGGACCATGAACTGGACCCTCGAAGGTGGAAGACATTAGAAACTCATGATGTTGTAAT
  AGAGTGTGCCAAAATCTCTCTGGACCCTACAGAAGCCTCGTATGAAGATGGCTATTCTGTGTCACACCAG
  CTCTCTGCTTGCTTCCCTTACCGTTCAGCTGATGTCACCACCAGCCCTTATGTGGACACACAGAATAGCT
  ATGGGGGTTCTACTTCCACCCCTTCCTCCAGCTCTCGGCTGATGTTGTTCAGTCCACCTGGGCAGCTACC
  TCAGAGTTTGAGTTCCCCATCAACACGGCTGTTACCTGAGCCGCTGCAAGCTAGTCTCTGGAGCCCATCT
  GCGGTCTGTGGTATGACCACTCCTCCTACGTCTCCTGGAAATGTCCCAGCTGATTTGTCACATCCGTATA
  GTAAAGCCTTTGGTACCACTGCAGGTGGAAAAGGAACTCCTTCAGGAACCCCAGCGACCTCTCCTCCTCC
  AGCCCCACCTTGTCCCCAAGATGACTGTGTGCATGGTTCAGCAGCCCAGGCCTCAGCCACAGCCCCCAGG
  AAGGAAGAAAGAGCAGATTCCTCAAGGCCTTACCTTCACAGACAGTCAAACGACCGAGGATTAGAGGATC
  CACCTGGAAGCAAAGGTTCCGTTACTCTGAGGAATCTACCTGATTTCCTAGGTGATCTGGCTTCTGAGGA
  AGACAGTATCGAGAAAGATAAGGAAGAAGCTGCAATATCTAAAGAGCTTTCTGAGATCACTACTGCAGAG
  GCGGATCCTGTAGTTCCTCGAGGGGGCTTTGACTCTCCCTTCTACCGAGACAGTCTCTCTGGCTCTCAGC
  GGAAGACTCATTCGGCAGCCTCTGGGACTCAGGGCTCCAGCGTGAACCCTGAGCCTTTGCACTCCTCCCT
  GGACAAACATGGGCCTGACACACCAAAGCAAGCCTTTACTCCCATAGACCCACCCTCTGGCAGTGCTGAT
  GTCAGTCCCGCTGGGGACAGGGATCGCCAGACTTCTCTGGAGACCAGTATCCTCACTCCCAGCCCTTGCA
  AAATCCCACCTCAGAGGGGAGTGAGCTTTGGAAGTGGGCAGCTTCCCCCATATGATCATCTCTTTGAGGT
  GGCCTTGCCAAAGACTGCCTGTCACTTTGTCAGCAAGAAGACTGAGGAGCTGTTGAAGAAAGTGAAAGGA
  AACCCTGAGGAAGACTGTGTGCCCTCTACCTCCCCAATGGAAGTACTGGACAGACTGATAGAGCAGGGAG
  CAGGTGCGCACAGCAAGGAGCTGAGCAGGTTGTCCCTGCCCAGCAAGTCTGTTGACTGGACCCACTTTGG
  AGGCTCTCCTCCCTCAGATGAGCTCCGGACCCTCCGAGACCAGTTGCTTTTACTGCACAATCAGCTGCTG
  TACGAGCGCTTCAAGCGGCAGCAGCATGCACTCAGGAACAGAAGGCTCCTGCGCAAGGTGATCCGAGCAG
  CGGCTCTGGAGGAACACAATGCAGCAATGAAAGATCAGTTGAAGTTACAAGAGAAGGACATACAGATGTG
  GAAGGTGAGTCTGCAGAAAGAACAAGCCCGATACAGTCAGCTTCAGGAACAACGTGACACCATGGTGACC
  CAACTGCATAGCCAGATCAGACAGCTACAGCATGACCGAGAAGAATTCTACAACCAGAGTCAGGAGTTAC
  AGACAAAGCTGGAGGACTGCAGAAACATGATTGCGGAGCTTCGGGTGGAGCTGAAGAAGGCTAACAACAA
  GGTGTGCCACACTGAGCTGCTGCTCAGCCAGGTCTCTCAGAAGCTCTCCAACAGTGAGTCAGTGCAGCAG
  CAGATGGAGTTCTTGAATAGGCAGCTCCTGGTGCTCGGGGAGGTCAATGAGCTGTACCTGGAGCAGCTGC
  AGAGCAAGCATCCTGACACCACCAAGGAAGTAGAAATGATGAAAACTGCATATCGGAAAGAGCTAGAGAA
  AAACAGAAGCCACCTTCTCCAGCAGAACCAAAGGTTGGACGCCTCACAGAGGCGAGTTTTGGAACTGGAG
  TCTCTTCTGGCCAAGAAAGACCACCTTCTCCTAGAACAGAAGAAATATCTTGAGGATGTCAAGAGCCAGG
  CGAGTGGACAGCTGCTGGCTGCAGAGAGCAGGTATGAGGCTCAGAGAAAGATCACCCGGGTGTTGGAACT
  GGAGATCCTAGACTTGTATGGCAGGTTGGAAAAAGATGGCCGCCTACGGAAACTAGAAGAGGACAGAGCA
  GAGGCAGCAGAGGCAGCAGAAGAGAGGCTTGACTGTTGTAGTGATGGATGCACAGATTCCTTGGTAGGAC
  ATAATGAAGAGGCTTCTGGTCACAATGGTGAGACCAGGACCTCCAGACCTGGTGGCACCCGGGCCAGCTG
  TGGAGGTAGAGTCACTGGAGGCAGCAGCAGCAGCAGCAGTGAGCTTTCCACTCCAGAGAAACCCCCGAGC
  CAGAGGTTCAGCAGCCGGTGGGAACCTGCCCTGGGCGAGCCCTCCAGCAGCATCCCCACCACTGTTGGCT
  CACTTCCCAGTTCCAAAAGCTTCCTGGGCATGAAGGCCCGGGAGCTGTTCCGTAATAAGAGCGAGAGCCA
  GTGTGATGAGGACAGCGTGACCATGAGTAGCAGCAGCCTTTCTGAGACCCTGAAGACAGAACTGGGCAAG
  GACTCGGGCACAGAAAACAAGACTTCCCTGAGTCTAGATGCCCCACACCCATCTTCCCCAAACTCAGACA
  ATGTGGGGCAGCTCCACATCATGGACTACAATGAGACTCATCCTGAACACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231634 representing NM_001289575
  Red=Cloning site Green=Tags(s)
  
  MAQLANIGELLSMLDSSTLGVRDDVTAIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDK
  HLLDKINEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITML
  PMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMK
  ENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGYSVSHQ
  LSACFPYRSADVTTSPYVDTQNSYGGSTSTPSSSSRLMLFSPPGQLPQSLSSPSTRLLPEPLQASLWSPS
  AVCGMTTPPTSPGNVPADLSHPYSKAFGTTAGGKGTPSGTPATSPPPAPPCPQDDCVHGSAAQASATAPR
  KEERADSSRPYLHRQSNDRGLEDPPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAE
  ADPVVPRGGFDSPFYRDSLSGSQRKTHSAASGTQGSSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSAD
  VSPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKVKG
  NPEEDCVPSTSPMEVLDRLIEQGAGAHSKELSRLSLPSKSVDWTHFGGSPPSDELRTLRDQLLLLHNQLL
  YERFKRQQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQEQRDTMVT
  QLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANNKVCHTELLLSQVSQKLSNSESVQQ
  QMEFLNRQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELE
  SLLAKKDHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLRKLEEDRA
  EAAEAAEERLDCCSDGCTDSLVGHNEEASGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPS
  QRFSSRWEPALGEPSSSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTMSSSSLSETLKTELGK
  DSGTENKTSLSLDAPHPSSPNSDNVGQLHIMDYNETHPEHS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289575
• ORF Size: 3483 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289575.1, NP_001276504.1
• RefSeq Size: 7732 bp
• RefSeq ORF: 3486 bp
• Locus ID: 64930
• UniProt ID: Q9EP53
• Cytogenetics: 2 A3
• MW: 129.2 kDa
• Gene Summary: In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (PubMed:16707451). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1 (PubMed:29127155). Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity (PubMed:29127155). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (By similarity). Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (By similarity).[UniProtKB/Swiss-Prot Function]