Nckap1 (NM_001290745) Mouse Tagged ORF Clone

CAT#: MR231612

Nckap1 (myc-DDK-tagged) - Mouse NCK-associated protein 1 (Nckap1), transcript variant 1

Product Images

Circular map for MR231612

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nckap1
• Synonyms: C79304; H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1; p125Nap1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231612 representing NM_001290745
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGCTCCGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTCAACGACCGGG
  GCGTCGGCATGCTCACGCGCCTCTACAACATCAAGAAGCAAGGACAAGTTTGGAAGGCATGTGGCGACCC
  CAAGGCTAAACCCTCCTACCTTATTGACAAAAACCTGGAATCTGCTGTGAAATTCATAGTCAGAAAATTT
  CCTGCTGTAGAAACGCGCAACAACAATCAACAGCTGGCACAGCTACAGAAAGAAAAATCAGAGATTCTGA
  AAAATCTGGCGTTGTATTACTTTACATTTGTAGATGTTATGGAATTTAAGGACCATGTCTGTGACTTGCT
  GAATACTATTGACGTTTGCCAAGTCTTCTTTGATATTACTGTGAACTTTGATTTAACAAAGAACTACTTA
  GACTTGACTGTAACCTATACAACATTAATGATACTGCTGTCCCGGATTGAAGAACGGAAGGCAATCATTG
  GACTATACAATTATGCACATGAAATGACCCATGGAGCAAGTGACCGAGAATATCCACGCCTTGGTCAGAT
  GATTGTGGACTATGAAAACCCTTTAAAAAAGATGATGGAAGAATTTGTACCCCATAGCAAGTCTCTTTCA
  GATGCGCTAATTTCTCTTCAGATGGTGTATCCTAGACGGAACCTTTCAGCAGACCAGTGGAGGAATGCCC
  AGTTGCTGAGTCTCATCAGTGCACCCAGCACAATGCTTAATCCTGCGCAGTCGGACACTATGCCTTGTGA
  ATATCTCTCCTTGGATGCAATGGAGAAATGGATCATCTTTGGCTTTATTCTGTGCCATGGGATGCTAAAT
  ACGGAGGCTACCGCACTGAACCTTTGGAAGCTAGCTCTTCAGAGTAGCTCTTGCCTCTCTCTCTTTCGGG
  ATGAAGTTTTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATTCGGGGCTACAATAAACGTAT
  TAACGACATAAGAGAATGCAAGGAGGCGGCTGTATCCCACGCCGGCTCAATGCACAGAGAAAGACGCAAG
  TTTTTACGGTCTGCACTAAAAGAACTGGCCACTGTCCTCTCTGACCAGCCTGGCCTGCTTGGTCCCAAGG
  CACTTTTTGTTTTCATGGCGTTATCTTTTGCCCGTGATGAAATTATCTGGCTGCTCCGTCACGCGGACAA
  CATGCCAAAGAAGAGTGCAGATGACTTTATAGATAAGCACATTGCTGAGCTAATATTTTACATGGAAGAG
  CTTAGAGCACATGTCAGGAAGTATGGGCCTGTCATGCAGAGGTACTACGTGCAATACCTCTCTGGCTTTG
  ATGCTGTTGTCCTCAATGAACTTGTGCAGAACCTGTCTGTTTGCCCTGAGGATGAATCGATCATCATGTC
  CTCTTTTGTGAACACCATGACCTCCCTAAGTGTGAAGCAAGTTGAAGATGGAGAAGTGTTTGATTTCAGA
  GGGATGAGATTAGATTGGTTTAGGTTACAGGCGTACACTAGCGTCTCCAAAGCTTCACTTAGCCTTGCAG
  ATCACAGAGAACTTGGAAAGATGATGAACACAATCATATTCCATACAAAAATGGTAGATTCCTTGGTTGA
  AATGTTGGTGGAAACATCCGATCTCTCCATATTTTGTTTTTACAGCCGTGCTTTTGAGAAGATGTTTCAG
  CAGTGCTTGGAGCTGCCCTCTCAGTCAAGATACTCCATTGCCTTTCCTCTGCTCTGCACTCACTTCATGA
  GCTGCACACATGAACTGTGTCCTGAAGAGCGCCATCATATAGGAGATCGTAGCCTTTCCCTGTGCAATAT
  GTTCCTGGATGAGATGGCCAAACAAGCTCGAAATCTCATCACCGATATTTGCACAGAACAGTGTACTCTT
  AGTGACCAGTTATTGCCGAAGCATTGTGCCAAAACCATCAGTCAAGCAGTGAATAAGAAGTCAAAAAAAC
  AGACGGGCAAGAAAGGGGAACCTGAAAGGGAAAAACCGGGTGTGGAGAGCATGAGGAAAAACAGGCTGGT
  AGTGACCAACCTTGATAAGTTGCACACTGCACTTTCGGAGTTATGCTTCTCCATAAATTATGTTCCAAAC
  ATGGCGGTGTGGGAGCACACCTTCACGCCGAGGGAGTATTTGACTTCTCATCTGGAAATCCGGTTCACTA
  AATCAATTGTTGGAATGACTATGTATAATCAAGCTACACAGGAAATTGCAAAGCCATCAGAGCTTCTAAC
  AAGCGTAAGAGCATATATGACTGTACTCCAGTCCATAGAGAACTATGTGCAGATTGATATCACCAGAGTA
  TTTAATAATGTCCTTCTTCAGCAAACACAACACTTAGACAGCCATGGAGAACCAACCATCACGAGTCTGT
  ATACAAACTGGTACTTGGAAACTTTATTAAGACAAGTCAGCAATGGCCATATAGCTTATTTTCCTGCAAT
  GAAAGCATTTGTGAATTTACCCACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGATATATCA
  GAAATGAGATCATTATCAGAGCTCCTAGGCCCATATGGGATGAAGTTCCTGAGTGAGAGCCTTATGTGGC
  ACATTTCATCACAAGTAGCTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGTTCTAACACAAATGAG
  GACCAGCTTTGACAAACCAGACCAGATGGCTGCACTCTTTAAAAGATTATCATCTGTTGACAGTGTCTTG
  AAAAGGATGACGATAATTGGTGTTATTTTATCCTTCCGGTCATTGGCACAAGAAGCATTGAGAGATGTCC
  TGTCCTACCACATTCCTTTCCTCGTGAGCTCCATTGAAGATTTCAAGGATCACATTCCTCGGGAAACTGA
  TATGAAGGTTGCAATGAATGTGTATGAGCTGTCCTCGGCTGCTGGGTTACCATGTGAGATTGATCCTGCC
  TTAGTCGTAGCTCTTTCCTCACAAAAATCAGAGAACATTAGTCCAGAGGAAGAATACAAGATCGCTTGTC
  TCCTGATGGTCTTTGTAGCAGTTTCCTTGCCTACACTGGCCAGCAATGTAATGTCACAGTACAGCCCTGC
  AATAGAAGGACACTGCAACAATATACATTGTTTGGCCAAAGCCATCAACCAGATTGCTGCAGCTTTGTTT
  ACAATTCACAAAGGAAGTATTGAAGACCGACTTAAGGAATTCCTGGCGCTTGCATCATCCAGTCTACTGA
  AAATTGGCCAAGAGACAGATAAAACTACAACAAGAAATAGAGAATCTGTTTATTTGCTACTGGATATGAT
  TGTACAAGAATCACCATTCCTGACAATGGATCTCTTGGAATCTTGTTTTCCTTATGTGTTGCTGAGAAAT
  GCATACCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231612 representing NM_001290745
  Red=Cloning site Green=Tags(s)
  
  MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKQGQVWKACGDPKAKPSYLIDKNLESAVKFIVRKF
  PAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCDLLNTIDVCQVFFDITVNFDLTKNYL
  DLTVTYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLS
  DALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGMLN
  TEATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRK
  FLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEE
  LRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFR
  GMRLDWFRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQ
  QCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTL
  SDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPN
  MAVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRV
  FNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDIS
  EMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVL
  KRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPA
  LVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALF
  TIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRN
  AYHAVYKQSVTSSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290745
• ORF Size: 3402 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290745.1, NP_001277674.1
• RefSeq Size: 4421 bp
• RefSeq ORF: 3405 bp
• Locus ID: 50884
• Cytogenetics: 2 48.21 cM
• MW: 130 kDa
• Gene Summary: Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).[UniProtKB/Swiss-Prot Function]