Uba1 (NM_001276317) Mouse Tagged ORF Clone

CAT#: MR231534

Uba1 (myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 1 (Uba1), transcript variant 4

Product Images

Circular map for MR231534

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Uba1
• Synonyms: A1; A1S9; Sb; Sbx; Ube-1; Ube1x
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231534 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAGCTCGCCGCTGTCCAAGAAACGTCGCGTGTCCGGGCCTGATCCAAAGCCGGGTTCTAACTGCT
  CCCCTGCACAGTCTGCGCTGTCCGAAGTGTCCTCAGTGCCAACCAACGGAATGGCGAAGAACGGCAGTGA
  AGCAGACATAGACGAGAGCCTTTACTCCCGGCAGCTGTACGTTTTGGGCCATGAGGCAATGAAAATGCTC
  CAGACATCCAGCGTCCTTGTCTCAGGCTTGCGGGGCTTGGGTGTAGAAATTGCTAAGAACATCATCCTTG
  GTGGGGTCAAGGCTGTCACCCTACATGACCAAGGAACTACCCAGTGGGCTGATCTCTCCTCCCAGTTTTA
  CCTTCGGGAGGAGGACATTGGTAAAAATCGAGCGGAGGTATCCCAGCCCCGACTTGCTGAACTCAACAGC
  TATGTACCTGTCACTGCCTACACTGGGCCTCTTGTCGAGGACTTCCTTAGTAGCTTCCAGGTGGTGGTCC
  TCACCAACAGCCCCCTGGAAGCCCAGCTGCGAGTGGGGGAGTTCTGTCATAGCCGTGGTATCAAGCTAGT
  GGTGGCAGATACAAGAGGCCTGTTTGGGCAACTTTTCTGTGATTTTGGAGAGGAAATGGTCCTCACAGAT
  TCCAATGGGGAGCAGCCACTCAGTGCTATGGTTTCAATGGTCACCAAGGACAACCCCGGTGTGGTTACCT
  GCCTTGATGAGGCCCGACATGGCTTTGAGACTGGTGACTTCGTCTCATTCTCAGAAGTACAGGGCATGAT
  CCAACTCAATGGATGTCAGCCCATGGAGATCAAAGTGCTGGGTCCTTATACCTTTAGTATCTGTGACACT
  TCCAACTTCTCTGACTACATCCGTGGAGGCATCGTCAGTCAGGTCAAAGTACCGAAAAAGATTAGTTTTA
  AATCCTTGCCAGCATCACTGGTAGAGCCTGACTTTGTGATGACTGACTTTGCCAAGTATTCTCGCCCTGC
  CCAACTGCACATTGGCTTCCAAGCTCTGCACCAATTCTGTGCTCTGCACAACCAACCACCTCGACCACGA
  AATGAGGAAGATGCAACAGAGCTGGTGGGCCTGGCTCAGGCTGTAAACGCTCGGTCCCCACCTTCAGTAA
  AACAGAACAGCTTGGATGAAGACCTTATTCGGAAGCTAGCTTATGTTGCTGCTGGGGACCTGGCACCCAT
  AAATGCTTTCATTGGGGGCCTTGCTGCCCAGGAAGTCATGAAGGCCTGCTCTGGAAAGTTTATGCCCATC
  ATGCAGTGGTTGTACTTTGATGCTCTTGAATGTCTCCCAGAGGACAAAGAGGCTCTGACAGAGGAGAAGT
  GCCTCCCACGTCAGAACCGTTACGATGGGCAGGTAGCTGTATTTGGGTCAGACTTTCAGGAGAAGCTGAG
  CAAGCAAAAGTACTTCCTGGTGGGTGCAGGGGCCATTGGCTGTGAATTGCTCAAGAACTTTGCCATGATT
  GGGCTGGGTTGTGGAGAGGGTGGAGAGGTCGTGGTCACAGACATGGACACCATTGAGAAATCAAATCTGA
  ACCGACAGTTTCTCTTCCGGCCCTGGGATGTCACGAAGTTAAAGTCTGACACGGCCGCTGCAGCTGTGCG
  CCAGATGAATCCTTACATCCAGGTGACAAGCCACCAGAACCGTGTAGGTCCTGACACTGAGCGCATCTAT
  GATGATGATTTCTTCCAAAATTTGGATGGTGTGGCCAATGCTCTGGACAACATAGATGCCCGCATGTACA
  TGGATCGCCGATGTGTGTACTATCGGAAGCCACTGCTAGAGTCTGGCACACTGGGCACAAAGGGCAACGT
  GCAGGTGGTAATCCCCTTCCTGACAGAATCCTACAGCTCTAGCCAGGATCCACCAGAGAAATCCATCCCC
  ATTTGTACCCTGAAAAACTTTCCCAATGCCATCGAACACACTCTTCAGTGGGCCCGGGATGAATTTGAAG
  GCCTTTTCAAGCAGCCAGCAGAAAATGTTAATCAGTACCTCACAGACTCCAAATTTGTGGAGCGGACATT
  GCGGCTGGCTGGTACCCAGCCATTGGAGGTGCTGGAGGCTGTGCAGCGCAGCCTGGTGTTGCAGCGACCA
  CAGACTTGGGGAGACTGTGTGACCTGGGCCTGCCACCACTGGCACACCCAGTACTGTAACAACATCCGGC
  AACTGCTGCACAACTTTCCTCCTGACCAGCTCACCAGCTCAGGGGCCCCTTTCTGGTCTGGACCCAAACG
  CTGTCCACACCCACTTACTTTTGATGTTAACAATACATTGCATCTGGATTATGTGATGGCTGCTGCCAAC
  CTTTTTGCCCAGACCTATGGGTTGACTGGATCCCAAGACCGAGCTGCTGTGGCCTCACTCCTGCAGTCAG
  TACAAGTCCCAGAGTTCACCCCCAAGTCTGGTGTCAAGATTCATGTTTCTGACCAGGAGTTGCAGAGTGC
  CAATGCCTCTGTTGATGACAGCCGTCTTGAGGAGCTCAAAGCCACATTGCCCAGCCCAGACAAGTTACCC
  GGATTTAAGATGTACCCCATTGATTTTGAGAAGGATGATGACAGCAATTTCCACATGGATTTCATTGTGG
  CTGCATCCAATCTTCGGGCCGAAAACTATGATATTTCCCCTGCAGACCGACACAAGAGCAAGCTGATTGC
  AGGGAAGATCATCCCAGCCATTGCCACAACCACAGCTGCTGTGGTTGGCCTTGTGTGTCTGGAGCTCTAC
  AAGGTAGTTCAAGGGCACCAACAGCTCGATTCCTACAAAAATGGTTTCCTGAACTTGGCCCTGCCCTTCT
  TTGGGTTTTCTGAACCTCTTGCTGCACCTCGTCACCAGTACTATAATCAAGAGTGGACATTGTGGGATCG
  CTTTGAAGTACAAGGGCTGCAGCCTAATGGTGAGGAGATGACCCTCAAGCAGTTCCTTGATTACTTTAAG
  ACAGAGCACAAATTGGAGATCACCATGCTGTCCCAGGGCGTGTCCATGCTCTATTCTTTCTTCATGCCAG
  CTGCTAAGCTCAAGGAACGATTGGATCAGCCGATGACAGAGATTGTGAGCCGAGTGTCAAAGAGAAAGCT
  GGGCCGCCATGTGCGGGCACTGGTGCTTGAGCTGTGCTGCAACGATGAAAGCGGCGAGGACGTCGAGGTC
  CCTTATGTCCGATATACCATTCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231534 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSSPLSKKRRVSGPDPKPGSNCSPAQSALSEVSSVPTNGMAKNGSEADIDESLYSRQLYVLGHEAMKML
  QTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNS
  YVPVTAYTGPLVEDFLSSFQVVVLTNSPLEAQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTD
  SNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMIQLNGCQPMEIKVLGPYTFSICDT
  SNFSDYIRGGIVSQVKVPKKISFKSLPASLVEPDFVMTDFAKYSRPAQLHIGFQALHQFCALHNQPPRPR
  NEEDATELVGLAQAVNARSPPSVKQNSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPI
  MQWLYFDALECLPEDKEALTEEKCLPRQNRYDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMI
  GLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIY
  DDDFFQNLDGVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIP
  ICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRP
  QTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAAN
  LFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLP
  GFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELY
  KVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFK
  TEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEV
  PYVRYTIR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276317
• ORF Size: 3177 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276317.1, NP_001263246.1
• RefSeq Size: 4068 bp
• RefSeq ORF: 3177 bp
• Locus ID: 22201
• UniProt ID: Q02053
• Cytogenetics: X 16.15 cM
• MW: 117.8 kDa
• Gene Summary: This gene encodes a member of the ubiquitin-activating E1 family. The encoded protein initiates the ubiquitin activation and transfer cascade, catalyzing the first step in ubiquitin conjugation to mark cellular proteins for proteasome degradation. Ubiquitin activating enzymes use ATP to form a thioester between a conserved catalytic cysteine of the enzyme and the C-terminal carboxylate of ubiquitin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]