Adgrg2 (NM_001290445) Mouse Tagged ORF Clone

CAT#: MR231467

Gpr64 (myc-DDK-tagged) - Mouse G protein-coupled receptor 64 (Gpr64), transcript variant 5

Product Images

Circular map for MR231467

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adgrg2
• Synonyms: AW212196; B830041D06Rik; Gpr64; Me6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231467 representing NM_001290445
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTTCTCTGGTGGGCAGTACAGCCCTGTTGGCAGACCTGAAGAGGTTTTACTGATATACAAGATAT
  TCCTTGTCATCATTTGTTTTCATGTCATTCTCGTTACATCCCTGAAAGAAAACGGTAATTCCAGTTTGTT
  ATCACCATCTGATGCTGCTTCAGAAGTTTTGTCGACTTTAAACAAAACAGAAAAATCTAAAATCACTATA
  GTAAAAACCTTCAATGCATCAGGAGTCAAATCCCAGAGAAATATCTGCAATTTGTCATCTCTTTGCAATG
  ACTCAGTATTTTTTAGAGGTGAGATAGTGTTTCAACATGATGAAGACCACAATGTTACCCAGAATCAAGA
  TACAGCTAATGGCACCTTCGCTGGAGTCCTGTCTCTAAGTGAACTGAAGCGATCAGAGCTCAACAAAACT
  CTACAGACCTTAAGTGAGACTTACTTTATAGTGTGTGCTACAGCAGAGGCCCAAAGCACGGTAAACTGTA
  CATTCACAGTAAAACTCAATGAGACCATGAATGTGTGTGCCATGATGGTTACTTTCCAAACTGTACAGAT
  TCGGCCAATGGAACAGTGCTGCTGTTCCCCGAGGACTCCCTGCCCTTCCTCACCAGAAGAGTTAGAAAAA
  CTACAGTGTGAACTGCAGGATCCCATTGTCTGTCTTGCTGATCAACCGCATGGCCCACCGTTATCGTCTT
  CCAGCAAGCCTGTTGTACCTCAGGCCACCATTATTTCCCATGTTGCTAGTGACTTCTCTTTGGCTGAACC
  CCTTGATCATGCCCTTATGACCCCAAGCACACCCTCTCTGACACAAGAAAGTAACCTTCCATCTCCTCAG
  CCTACGATCCCCCTGGCTTCCAGTCCTGCCACTGACTTGCCAGTTCAATCTGTAGTGGTCTCTTCTTTGC
  CTCAAACTGATCTTTCCCACACCCTGTCACCGGTGCAGTCCTCCATTCCCTCTCCTACCACACCAGCCCC
  ATCTGTCCCTACAGAACTGGTCACCATCAGCACACCTCCTGGTGAGACAGTTGTCAACACTAGCACTGTT
  TCTGATCTGGAAGCCCAAGTATCCCAGATGGAGAAAGCTTTGTCTTTGGGTAGCTTAGAGCCTAATCTTG
  CAGGCGAAATGGTAAACCGAGTCAGCAAACTCCTTCACTCTCCACCTGCCTTGCTAGCCCCTCTAGCTCA
  AAGGTTGCTAAAAGTGGTAGATGCCATTGGCTTACAGCTGAATTTTTCATCTACAACTATCAGTCTAACT
  TCACCTTCTTTGGCTCTTGCTGTGATCAGAGTGAATGCCAGTAATTTCAATACCACGACTTTTGCAGCCC
  AAGACCCAACAAATCTCCAGGTTTCTCTGGAAACCCCACCTCCTGAGAATAGTATTGGTGCCATTACTCT
  GCCCTCATCACTGATGAATAATTTGCCAGCTAATGATGTAGAATTGGCTTCAAGGATTCAGTTCAATTTC
  TTTGAAACACCCGCCCTGTTTCAGGATCCTTCCCTGGAGAACCTCACTCTGATAAGCTATGTCATATCAT
  CAAGTGTCACAAACATGACCATCAAGAACTTGACAAGAAACGTGACAGTCGCACTGAAACACATCAACCC
  AAGTCCGGATGACTTAACTGTGAAATGTGTATTCTGGGACTTGGGCAGAAATGGTGGCAAAGGAGGTTGG
  TCATCTGATGGCTGTTCCGTCAAAGACAAGAGAATGAATGAAACCATCTGTACCTGTAGCCATCTTACAA
  GTTTTGGCATCCTATTGGACCTATCTCGAACATCCTTACCACCAAGTCAAATGATGGCTCTGACATTTAT
  CACGTATATTGGCTGTGGGCTTTCATCAATTTTTCTGTCAGTTACTCTTGTAACCTACATCGCCTTTGAA
  AAGATCCGGAGGGATTACCCCTCCAAAATCCTCATCCAGCTGTGTGCTGCCCTGCTTCTGCTCAACCTGA
  TCTTCCTCCTAGACTCCTGGATTGCGCTGTATAATACCCGAGGTTTCTGCATTGCCGTGGCTGTATTTCT
  TCACTATTTTCTCTTGGTCTCATTCACATGGATGGGATTAGAAGCATTCCACATGTACCTAGCACTGGTC
  AAGGTGTTTAATACTTACATCCGAAAGTACATCCTTAAATTCTGCATTGTTGGCTGGGGCATACCAGCTG
  TGGTTGTGTCCATCGTCCTGACTATATCCCCAGATAACTATGGGATTGGATCCTATGGAAAATTCCCCAA
  TGGCACACCAGATGACTTTTGCTGGATCAACAGCAATGTGGTGTTCTATATCACGGTTGTGGGATATTTC
  TGTGTGATATTTCTACTGAACGTCAGCATGTTCATCGTGGTCTTGGTTCAGCTCTGTCGAATTAAAAAGA
  AGAAGCAGCTGGGAGCCCAGCGCAAAACTAGTATTCAAGACCTCAGGAGTATCGCTGGCCTCACATTTTT
  ACTGGGAATTACTTGGGGCTTTGCCTTCTTTGCCTGGGGACCAGTTAATGTCACCTTCATGTATCTCTTT
  GCCATCTTTAACACCTTACAAGGGTTTTTCATATTCATTTTTTACTGTGCAGCAAAAGAGAATGTCAGAA
  AACAGTGGAGGCGGTATCTTTGTTGTGGAAAATTACGGCTGGCTGAAAATTCTGACTGGAGTAAAACTGC
  TACTAATGGTTTAAAGAAGCAGACTGTAAACCAAGGAGTATCCAGCTCTTCAAATTCCTTACAGTCAAGC
  TGTAACTCCACTAACTCCACCACACTCCTAGTGAATAGTGATTGCTCAGTGCACGCAAGCGGGAATGGCA
  ATGCATCTACCGAGAGGAATGGGGTTTCTTTCAGTGTTCAGAATGGAGACGTGTGCCTTCATGATCTCAC
  TGGCAAACAGCATATGTTTAGTGACAAAGAGGACTCCTGCAATGGTAAAAGCCGGATAGCGCTCAGAAGG
  ACTTCGAAGCGGGGAAGCTTACACTTTATCGAGCAAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231467 representing NM_001290445
  Red=Cloning site Green=Tags(s)
  
  MLFSGGQYSPVGRPEEVLLIYKIFLVIICFHVILVTSLKENGNSSLLSPSDAASEVLSTLNKTEKSKITI
  VKTFNASGVKSQRNICNLSSLCNDSVFFRGEIVFQHDEDHNVTQNQDTANGTFAGVLSLSELKRSELNKT
  LQTLSETYFIVCATAEAQSTVNCTFTVKLNETMNVCAMMVTFQTVQIRPMEQCCCSPRTPCPSSPEELEK
  LQCELQDPIVCLADQPHGPPLSSSSKPVVPQATIISHVASDFSLAEPLDHALMTPSTPSLTQESNLPSPQ
  PTIPLASSPATDLPVQSVVVSSLPQTDLSHTLSPVQSSIPSPTTPAPSVPTELVTISTPPGETVVNTSTV
  SDLEAQVSQMEKALSLGSLEPNLAGEMVNRVSKLLHSPPALLAPLAQRLLKVVDAIGLQLNFSSTTISLT
  SPSLALAVIRVNASNFNTTTFAAQDPTNLQVSLETPPPENSIGAITLPSSLMNNLPANDVELASRIQFNF
  FETPALFQDPSLENLTLISYVISSSVTNMTIKNLTRNVTVALKHINPSPDDLTVKCVFWDLGRNGGKGGW
  SSDGCSVKDKRMNETICTCSHLTSFGILLDLSRTSLPPSQMMALTFITYIGCGLSSIFLSVTLVTYIAFE
  KIRRDYPSKILIQLCAALLLLNLIFLLDSWIALYNTRGFCIAVAVFLHYFLLVSFTWMGLEAFHMYLALV
  KVFNTYIRKYILKFCIVGWGIPAVVVSIVLTISPDNYGIGSYGKFPNGTPDDFCWINSNVVFYITVVGYF
  CVIFLLNVSMFIVVLVQLCRIKKKKQLGAQRKTSIQDLRSIAGLTFLLGITWGFAFFAWGPVNVTFMYLF
  AIFNTLQGFFIFIFYCAAKENVRKQWRRYLCCGKLRLAENSDWSKTATNGLKKQTVNQGVSSSSNSLQSS
  CNSTNSTTLLVNSDCSVHASGNGNASTERNGVSFSVQNGDVCLHDLTGKQHMFSDKEDSCNGKSRIALRR
  TSKRGSLHFIEQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001290445
• ORF Size: 2979 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290445.1, NP_001277374.1
• RefSeq Size: 4675 bp
• RefSeq ORF: 2982 bp
• Locus ID: 237175
• UniProt ID: Q8CJ12
• Cytogenetics: X F4
• MW: 109 kDa
• Gene Summary: Orphan receptor. Could be involved in a signal transduction pathway controlling epididymal function and male fertility. May regulate fluid exchange within epididymis.[UniProtKB/Swiss-Prot Function]