Snap91 (NM_001277986) Mouse Tagged ORF Clone

CAT#: MR231344

Snap91 (myc-DDK-tagged) - Mouse synaptosomal-associated protein 91 (Snap91), transcript variant 2

Product Images

Circular map for MR231344

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Snap91
• Synonyms: 91kDa; AP180; F1-20; mKIAA0656
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231344 representing NM_001277986
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGCCAAACGCTCACGGATCGGATCGCCGCCGCTCAGTACAGCGTGACTGGCTCTGCTGTAGCAA
  GAGCAGTCTGCAAAGCCACCACTCATGAGGTGATGGGCCCCAAGAAGAAGCACCTGGACTATTTGATCCA
  GGCTACCAATGAGACCAATGTCAATATCCCTCAGATGGCCGACACCCTCTTTGAGCGGGCGACAAACAGT
  AGCTGGGTGGTGGTATTTAAAGCTTTAGTGACCACACACCATCTCATGGTGCATGGAAATGAGAGATTTA
  TTCAGTATTTGGCCTCTAGGAATACGCTATTCAATCTCAGCAACTTTCTAGATAAAAGTGGATCCCACGG
  TTATGATATGTCTACGTTCATACGGCGTTACAGTAGATACTTGAATGAAAAGGCTTTCTCCTACAGACAG
  ATGGCATTTGACTTTGCCAGAGTGAAGAAAGGGGCTGACGGTGTCATGAGGACGATGGTTCCTGAAAAGC
  TCCTGAAGAGTATGCCAATCCTGCAGGGGCAGATCGATGCACTGCTGGAGTTTGATGTGCATCCAAATGA
  ACTAACCAATGGTGTCATAAATGCTGCATTTATGCTTCTTTTCAAAGATCTTATCAAACTGTTTGCTTGC
  TACAATGACGGCGTCATTAACTTACTTGAAAAATTTTTCGAGATGAAGAAAGGTCAATGCAAAGACGCGC
  TAGAAATTTACAAGCGATTTCTAACTAGAATGACGAGGGTGTCTGAATTCCTCAAGGTCGCCGAGCAAGT
  TGGTATTGATAAAGGTGACATTCCCGACCTCACACAGGCTCCCAGCAGTCTTATGGAGACCCTTGAACAA
  CATCTAAATACCCTAGAAGGAAAGAAACCTGGAAACAATGAAGGATCTGGTGCTCCCTCTCCACTAAGTA
  AGTCTTCTCCAGCCACAACTGTTACATCTCCTAATTCTACACCAGCTAAAACTATCGACACATCCCCGCC
  AGTTGACATATTTGCAACAGCATCCGCGGCTGCCCCAGTCAGCTCTGCTAAGCCATCAAGCGATCTCCTT
  GATCTTCAGCCCGACTTCTCTGGAGCAGCTGCGGGGGCAGCAGCACCTGTAGTGCCTCCTTCTGGGGGTG
  CGACCGCCTGGGGAGACCTTTTGGGAGAGGATTCCTTGGCTGCACTTTCCTCTGTTCCCTGTGAAGCACC
  GATTTCAGACCCATTTGCACCAGAGCCTTCCCCTCCTACTACAACCACTGAGCCTGCTTCAGCCTCTGCC
  TCGACCACCACAGCTGTGACGGCTGTCACTACGGAAGTGGATCTCTTTGGAGATGCCTTTGCAGCTTCTC
  CTGGGGAGGCCCCTGCAGCATCCGAAGGGGCTACCGCACCAGCTACCCCGGCCCCAGTGGCTGCAGCTCT
  TGATGCATGCTCAGGAAATGACCCTTTTGCCCCATCTGAAGGTAGCGCAGAGGCTGCACCTGAGCTGGAC
  CTCTTTGCAATGAAGCCACCTGAGACCAGCGCTCCTGTAGTTACCCCTACAGCTAGCACAGCCCCTCCAG
  TTCCCGCAACTGCTCCTTCTCCTGCTCCCACGGCTGTGGCAGCCACTGCTGCCACCACCACCGCCGCCGC
  TGCAGCTACCACCACTGCCACCACCTCTGCTGCTGCTGCCACCACCGCCGCTGCTCCTCCTGCTCTAGAT
  ATCTTTGGTGATTTGTTTGATTCTGCTCCTGAAGTTGCTGCAGCACCTAAGCCAGACGCGGCTCCTAGCA
  TAGACCTGTTTGGCACAGATGCTTTCTCCTCCCCGCCACGAGGGGCCTCTCCGGTGCCTGAGAGTTCTCT
  CACTGCTGACCTCTTATCTGTGGACGCATTTGCAGCGCCGTCTCCTGCATCCACTGCCTCTCCTGCAAAG
  GCGGAGTCCTCGGGTGTCATAGACCTTTTTGGGGATGCGTTTGGAAGTGGTGCTTCTGAAACCCAGCCAG
  CACCACAGGCTGTTTCTAGTTCATCAGCATCGGCAGATCTACTAGCTGGATTTGGGGGTTCTTTCATGGC
  CCCTTCTACAACGCCAGTGACTCCAGCTCAGAATAACCTGCTGCAACCCAGTTTCGAGGCAGCTTTTGGA
  ACGACGCCTTCGACTTCAAGCAGCAGCTCTTTTGACCCATCAGGCGATCTTCTGATGCCAACCATGGCAC
  CATCCGGGCAGCCTGCCCCTGTCTCAATGGTCCCACCCAGTCCTGCAATGGCAGCCAGCAAAGGCCTCGG
  AAGTGACCTTGACTCGTCTCTGGCCAGTTTAGTAGGCAATCTTGGGATTTCTGGTACCACATCAAAAAAG
  GGAGATCTCCAGTGGAATGCTGGGGAGAAAAAGCTGACTGGTGGAGCCAACTGGCAACCGAAAGTCACTC
  CAGCCACATGGTCAGCGGGTGTTCCCCCGCAAGGCACTGTTCCACCAACCAGCTCAGTCCCTCCGGGTGC
  CGGGGCCCCGTCCGTTGGGCAACCTGGAGCAGGATTTGGAATGCCTCCTTCAGGGACAGGCATGACCATG
  ATGTCTCAGCAGCCAGTCATGTTTGCACAGCCCATGATGAGGCCACCCTTTGGAGCTGCAGCTGTGCCCG
  GCACACAGCTTTCTCCAAGCCCTACACCTGCCACTCAGAGTCCCAAGAAACCTCCAGCCAAGGACCCGTT
  AGCGGATCTTAACATCAAGGATTTCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231344 representing NM_001277986
  Red=Cloning site Green=Tags(s)
  
  MSGQTLTDRIAAAQYSVTGSAVARAVCKATTHEVMGPKKKHLDYLIQATNETNVNIPQMADTLFERATNS
  SWVVVFKALVTTHHLMVHGNERFIQYLASRNTLFNLSNFLDKSGSHGYDMSTFIRRYSRYLNEKAFSYRQ
  MAFDFARVKKGADGVMRTMVPEKLLKSMPILQGQIDALLEFDVHPNELTNGVINAAFMLLFKDLIKLFAC
  YNDGVINLLEKFFEMKKGQCKDALEIYKRFLTRMTRVSEFLKVAEQVGIDKGDIPDLTQAPSSLMETLEQ
  HLNTLEGKKPGNNEGSGAPSPLSKSSPATTVTSPNSTPAKTIDTSPPVDIFATASAAAPVSSAKPSSDLL
  DLQPDFSGAAAGAAAPVVPPSGGATAWGDLLGEDSLAALSSVPCEAPISDPFAPEPSPPTTTTEPASASA
  STTTAVTAVTTEVDLFGDAFAASPGEAPAASEGATAPATPAPVAAALDACSGNDPFAPSEGSAEAAPELD
  LFAMKPPETSAPVVTPTASTAPPVPATAPSPAPTAVAATAATTTAAAAATTTATTSAAAATTAAAPPALD
  IFGDLFDSAPEVAAAPKPDAAPSIDLFGTDAFSSPPRGASPVPESSLTADLLSVDAFAAPSPASTASPAK
  AESSGVIDLFGDAFGSGASETQPAPQAVSSSSASADLLAGFGGSFMAPSTTPVTPAQNNLLQPSFEAAFG
  TTPSTSSSSSFDPSGDLLMPTMAPSGQPAPVSMVPPSPAMAASKGLGSDLDSSLASLVGNLGISGTTSKK
  GDLQWNAGEKKLTGGANWQPKVTPATWSAGVPPQGTVPPTSSVPPGAGAPSVGQPGAGFGMPPSGTGMTM
  MSQQPVMFAQPMMRPPFGAAAVPGTQLSPSPTPATQSPKKPPAKDPLADLNIKDFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277986
• ORF Size: 2688 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277986.1, NP_001264915.1
• RefSeq Size: 4353 bp
• RefSeq ORF: 2691 bp
• Locus ID: 20616
• UniProt ID: Q61548
• Cytogenetics: 9 E3.1
• MW: 91.8 kDa
• Gene Summary: Adaptins are components of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats.[UniProtKB/Swiss-Prot Function]