Cyld (NM_001276279) Mouse Tagged ORF Clone

CAT#: MR231117

Cyld (myc-DDK-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld), transcript variant 5

Product Images

Circular map for MR231117

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cyld
• Synonyms: 2010013M14Rik; 2900009M21Rik; C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231117 representing NM_001276279
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCAGGCCTGTGGAGCCAAGAGAAAGTTACTTCACCCTACTGGGAAGAACGGATTTTTTATCTGC
  TTCTTCAAGAATGCAGTGTAACAGACAAACAAACTCAGAAGCTGCTGAAAGTACCCAAAGGGAGTATAGG
  ACAGTACATCCAAGACCGTTCTGTGGGGCATTCAAGAGTTCCTTCCACAAAAGGCAAGAAAAATCAGATT
  GGATTAAAAATCTTGGAGCAACCGCATGCAGTTCTGTTTGTTGATGAAAAGGATGTTGTAGAAATAAATG
  AAAAATTCACAGAGTTACTGTTGGCAATTACCAACTGTGAGGAGAGGCTCAGCCTATTTAGAAACAGACT
  CCGACTAAGTAAAGGCCTCCAGGTAGACGTGGGCAGTCCTGTGAAAGTACAGCTGCGATCTGGGGAAGAG
  AAATTTCCAGGAGTTGTACGCTTCAGAGGACCTTTATTAGCGGAGAGGACGGTGTCGGGGATTTTCTTTG
  GAGTAGAATTATTGGAAGAAGGTCGTGGTCAAGGTTTCACGGATGGGGTATATCAAGGGAAGCAGCTTTT
  CCAGTGTGATGAGGACTGTGGCGTGTTTGTTGCATTGGACAAACTAGAACTTATAGAAGATGATGACAAT
  GGATTGGAAAGTGATTTTGCAGGCCCAGGAGATACAATGCAGGTTGAACCTCCCCCTTTGGAAATAAACT
  CCAGAGTTTCTTTGAAGGTTGGAGAAAGTACAGAATCTGGAACAGTAATATTCTGTGATGTTTTACCAGG
  AAAAGAGAGTCTAGGATATTTTGTTGGTGTGGACATGGATAACCCTATTGGCAACTGGGATGGAAGGTTT
  GATGGAGTACAGCTCTGTAGTTTTGCAAGTGTTGAAAGTACAATTCTCCTGCACATCAATGACATCATCC
  CAGATAGCGTGACACAGGAAAGGAGGCCTCCCAAACTTGCCTTTATGTCAAGAGGTGTAGGTGACAAAGG
  TTCATCTAGTCATAATAAACCAAAGGTTACAGGATCTACCTCAGACCCTGGAAGTAGAAACAGATCTGAA
  TTATTTTATACCTTAAATGGGTCATCTGTTGACTCACAACAATCCAAGTCCAAAAATCCATGGTACATTG
  ATGAAGCATTTGGGGGCTATTTAAGTGAAGTAGTAGAAGAAAATACTCCACCTAAAATGGAAAAGGAAGG
  TTTAGAGATAATGATTGGAAAGAAGAAAGGCATCCAGGGCCATTACAATTCTTGTTACTTAGACTCAACT
  TTATTCTGCTTATTTGCTTTTAGTTCTGCCCTGGACACTGTGTTACTTAGACCCAAAGAGAAGAATGATA
  TAGAGTATTACAGTGAGACTCAGGAGCTACTGAGGACAGAGATAGTCAATCCTCTGAGAATATATGGATA
  TGTGTGTGCCACAAAGATTATGAAACTGAGGAAAATACTTGAAAAAGTTGAGGCTGCATCAGGTTTTACC
  TCTGAAGAAAAAGATCCTGAAGAATTCTTAAATATCCTGTTTCATGATATTTTAAGGGTTGAACCATTGT
  TAAAAATAAGATCAGCAGGTCAAAAAGTTCAAGACTGTAACTTCTATCAAATTTTTATGGAAAAAAATGA
  GAAAGTTGGAGTACCCACAATTCAGCAGTTATTAGAATGGTCTTTTATCAACAGCAACCTGAAATTTGCA
  GAGGCACCATCATGCTTGATTATCCAGATGCCTCGGTTTGGAAAAGACTTTAAACTATTTAAAAAAATTT
  TTCCTTCCCTGGAATTAAATATAACAGATTTACTTGAAGACACTCCCAGGCAGTGCCGCATCTGTGGAGG
  ACTTGCGATGTACGAGTGCAGGGAGTGCTATGACGATCCGGACATCTCAGCTGGGAAGATCAAGCAGTTC
  TGTAAGACCTGCAGCACTCAGGTTCACCTTCATCCCAGGAGGTTGAATCATTCTTATCATCCAGTATCAC
  TTCCCAAAGACTTGCCTGACTGGGACTGGAGACACGGCTGCATCCCCTGTCAGAAGATGGAGTTATTTGC
  TGTTCTCTGCATAGAAACGAGCCACTATGTTGCTTTTGTGAAGTATGGGAAGGATGACTCTGCCTGGCTT
  TTCTTTGACAGCATGGCGGATCGAGATGGTGGTCAGAATGGCTTCAACATTCCACAAGTGACGCCCTGCC
  CAGAAGTGGGAGAGTACTTGAAGATGTCTCTGGAGGACCTGCACTCTTTGGATTCCAGAAGGATTCAAGG
  CTGTGCGCGCAGACTTCTTTGCGATGCATACATGTGCATGTACCAGAGTCCAACCATGAGCCTGTACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231117 representing NM_001276279
  Red=Cloning site Green=Tags(s)
  
  MSSGLWSQEKVTSPYWEERIFYLLLQECSVTDKQTQKLLKVPKGSIGQYIQDRSVGHSRVPSTKGKKNQI
  GLKILEQPHAVLFVDEKDVVEINEKFTELLLAITNCEERLSLFRNRLRLSKGLQVDVGSPVKVQLRSGEE
  KFPGVVRFRGPLLAERTVSGIFFGVELLEEGRGQGFTDGVYQGKQLFQCDEDCGVFVALDKLELIEDDDN
  GLESDFAGPGDTMQVEPPPLEINSRVSLKVGESTESGTVIFCDVLPGKESLGYFVGVDMDNPIGNWDGRF
  DGVQLCSFASVESTILLHINDIIPDSVTQERRPPKLAFMSRGVGDKGSSSHNKPKVTGSTSDPGSRNRSE
  LFYTLNGSSVDSQQSKSKNPWYIDEAFGGYLSEVVEENTPPKMEKEGLEIMIGKKKGIQGHYNSCYLDST
  LFCLFAFSSALDTVLLRPKEKNDIEYYSETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFT
  SEEKDPEEFLNILFHDILRVEPLLKIRSAGQKVQDCNFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFA
  EAPSCLIIQMPRFGKDFKLFKKIFPSLELNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQF
  CKTCSTQVHLHPRRLNHSYHPVSLPKDLPDWDWRHGCIPCQKMELFAVLCIETSHYVAFVKYGKDDSAWL
  FFDSMADRDGGQNGFNIPQVTPCPEVGEYLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276279
• ORF Size: 2310 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276279.1, NP_001263208.1
• RefSeq Size: 7599 bp
• RefSeq ORF: 2313 bp
• Locus ID: 74256
• Cytogenetics: 8 C3
• MW: 87.5 kDa
• Gene Summary: This gene encodes a protein that is a member of the ubiquitin C-terminal hydrolase subfamily of the deubiquitinating enzyme family. Members of this family catalyze the removal of ubiquitin from a substrate or another ubiquitin molecule and thereby play important roles in regulating signaling pathways, recycling ubiquitin and regulating protein stability. This protein removes ubiquitin from K-63-linked ubiquitin chains from proteins involved in NF-kappaB signaling and thus acts as a negative regulator of this pathway. In humans mutations in this gene have been associated with cylindromatosis, an autosomal dominant predisposition to tumors of skin appendages. In mouse deficiency of this gene impairs thymocyte development and increases susceptibility to skin and colon tumors. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]