Sec23b (NM_001252544) Mouse Tagged ORF Clone

CAT#: MR231104

Sec23b (myc-DDK-tagged) - Mouse SEC23B (S. cerevisiae) (Sec23b), transcript variant 3

Product Images

Circular map for MR231104

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sec23b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231104 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAACGTATCTGGAATTCATTCAGCAGAATGAAGAACGAGATGGTGTGCGTTTCAGTTGGAACGTGT
  GGCCTTCTAGCCGCCTTGAAGCCACAAGAATGGTTGTGCCCTTGGCTTGTCTCCTCACTCCTTTAAAAGA
  ACGCCCAGACTTACCTCCTGTACAGTATGAACCTGTGCTTTGCAGTAGGCCTACCTGCAAAGCTATTCTC
  AATCCACTTTGTCAAGTGGATTACCGAGCAAAACTCTGGGCCTGCAATTTCTGTTTTCAAAGAAATCAGT
  TCCCTCCAGCATATGCAGGCATATCTGAGGTGAATCAACCTGCTGAATTGATGCCCCAGTTCTCCACAAT
  TGAGTACATGATCCAGAGAGGTGCTCGGTCTCCTCTGATCTTTCTCTATGTGGTTGACACGTGTTTGGAG
  GAAGATGACCTTCAAGCACTCAAAGAGTCCCTGCAGATGTCTCTGAGTCTCCTTCCTCCAGATGCCCTGG
  TTGGTCTCATCACTTTTGGGAGGATGGTGCAGGTTCATGAGCTAAGCTGTGAAGGAATCTCCAAGAGCTA
  TGTCTTCCGAGGGACCAAGGATTTAACTGCAAAGCAAATACAGGAGATGCTGGGCCTGACCAAGTCAGCC
  ATGCCTGTGCAGCAAGCCAGACCTGCTCAACCTCAGGAGCAGCCTTTTGTTTCCAGCAGATTTCTACAGC
  CCATTCACAAGATTGACATGAACCTCACAGATCTTCTCGGGGAGCTGCAGAGGGACCCATGGCCAGTAAC
  TCAGGGGAAGAGACCACTGCGGTCCACTGGTGTTGCTTTGTCCATTGCTGTTGGCTTGTTGGAGGGGACT
  TTCCCAAATACTGGAGCCAGGATCATGCTGTTCACTGGCGGGCCCCCGACCCAAGGACCTGGCATGGTGG
  TTGGAGATGAGTTGAAGACTCCTATTCGTTCCTGGCACGACATTGAGAAAGATAACGCACGATTCATGAA
  GAAGGCAACCAAGCATTATGAGATGCTTGCTAATCGAACTGCTACAAATGGCCACTGCATTGATATTTAT
  GCTTGTGCCCTTGACCAAACTGGACTTCTGGAGATGAAGTGCTGTCCAAATCTTACAGGAGGCCACATGG
  TGATGGGAGATTCTTTCAACACTTCTCTTTTCAAGCAGACATTCCAAAGAATTTTTAGTAAAGACTTCAA
  CGGAGATTTCAGAATGGCGTTTGGTGCTACGTTGGATGTAAAGACCTCTCGGGAACTGAAGATTGCAGGA
  GCCATTGGTCCATGCGTGTCTCTGAATGTGAAAGGGCCATGCGTGTCAGAAAATGAGCTTGGTGTTGGTG
  GCACAAGTCAGTGGAAGATCTGTGGCCTGGATCCCTCATCTACTCTTGGCATCTACTTTGAAGTTGTCAA
  TCAGCACAATGCTCCAGTTCCCCAGGGAGGCAGAGGTGCCATCCAGTTTGTCACACAGTATCAGCATTCC
  AGCACTCAGAAGCGCATCCGAGTGACCACCATTGCCCGCAATTGGGCAGATGCACAGAGTCAGCTGAGGC
  ACATAGAAGCAGCATTTGACCAGGAGGCCGCAGCAGTCCTGATGGCACGGCTTGGAGTGTTCCGCGCAGA
  GTCAGAGGAGGGGCCTGACGTGCTTCGGTGGCTGGATCGGCAACTCATCAGACTGTGTCAGAAGTTTGGG
  CAGTATAACAAAGAAGACCCTACGTCTTTCAGATTGTCAGACTCCTTTTCTCTGTATCCTCAGTTCATGT
  TCCATCTGAGAAGATCTCCATTTCTTCAAGTATTTAACAACAGTCCTGATGAGTCCTCCTATTATAGACA
  CCATTTTGCCCGGCAGGATCTGACTCAGTCCCTCATTATGATCCAGCCCATTCTCTATTCTTACTCCTTC
  CACGGACCACCAGAGCCTGTACTCCTGGACAGCAGTAGCATTCTAGCTGACAGAATTCTGCTGATGGATA
  CTTTCTTCCAAATTGTCATTTACCTTGGTGAGACCATAGCCCAGTGGCGGAAGGCTGGATACCAGGACAT
  GCCCGAGTATGAAAACTTCAAGCACCTTCTTCAGGCGCCCCTGGATGATGCCCAAGAAATTCTGCAAGCA
  CGCTTCCCAATGCCACGTTATATCAACACAGAGCATGGCGGCAGTCAGGCTCGGTTCCTGTTGTCTAAAG
  TTAACCCATCTCAGACACACAATAACCTGTATGCCTGGGGGCAGGAAACTGGAGCGCCCATCCTGACAGA
  CGATGTCAGCCTACAGGTGTTCATGGATCACCTGAAGAAGCTGGCTGTGTCTAGTGCCTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231104 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATYLEFIQQNEERDGVRFSWNVWPSSRLEATRMVVPLACLLTPLKERPDLPPVQYEPVLCSRPTCKAIL
  NPLCQVDYRAKLWACNFCFQRNQFPPAYAGISEVNQPAELMPQFSTIEYMIQRGARSPLIFLYVVDTCLE
  EDDLQALKESLQMSLSLLPPDALVGLITFGRMVQVHELSCEGISKSYVFRGTKDLTAKQIQEMLGLTKSA
  MPVQQARPAQPQEQPFVSSRFLQPIHKIDMNLTDLLGELQRDPWPVTQGKRPLRSTGVALSIAVGLLEGT
  FPNTGARIMLFTGGPPTQGPGMVVGDELKTPIRSWHDIEKDNARFMKKATKHYEMLANRTATNGHCIDIY
  ACALDQTGLLEMKCCPNLTGGHMVMGDSFNTSLFKQTFQRIFSKDFNGDFRMAFGATLDVKTSRELKIAG
  AIGPCVSLNVKGPCVSENELGVGGTSQWKICGLDPSSTLGIYFEVVNQHNAPVPQGGRGAIQFVTQYQHS
  STQKRIRVTTIARNWADAQSQLRHIEAAFDQEAAAVLMARLGVFRAESEEGPDVLRWLDRQLIRLCQKFG
  QYNKEDPTSFRLSDSFSLYPQFMFHLRRSPFLQVFNNSPDESSYYRHHFARQDLTQSLIMIQPILYSYSF
  HGPPEPVLLDSSSILADRILLMDTFFQIVIYLGETIAQWRKAGYQDMPEYENFKHLLQAPLDDAQEILQA
  RFPMPRYINTEHGGSQARFLLSKVNPSQTHNNLYAWGQETGAPILTDDVSLQVFMDHLKKLAVSSAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252544
• ORF Size: 2301 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252544.1, NP_001239473.1
• RefSeq Size: 2792 bp
• RefSeq ORF: 2304 bp
• Locus ID: 27054
• UniProt ID: Q9D662
• Cytogenetics: 2 G1
• MW: 86.4 kDa
• Gene Summary: Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex.[UniProtKB/Swiss-Prot Function]