Sun2 (NM_001205345) Mouse Tagged ORF Clone

CAT#: MR231010

Sun2 (myc-DDK-tagged) - Mouse Sad1 and UNC84 domain containing 2 (Sun2), transcript variant 1

Product Images

Circular map for MR231010

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sun2
• Synonyms: B230369L08Rik; C030011B15; Unc84b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR231010 representing NM_001205345
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAGACGAAGCCAGCGCCTCACTCGCTACTCTCAGGATGATAACGATGGCGGCAGCAGCAGCAGTG
  GTGCGAGCTCCGTGGCAGGAAGCCAGGGCACCGTGTTTAAAGACAGTCCTCTCAGGACTTTGAAGAGGAA
  ATCCAGCAACATGAAGCACCTGTCCCCAGCTCCACAGCTGGGCCCCTCCTCTGACTCCCACACCTCCTAC
  TACAGCGAGTCTGTGGTTCGAGAGTCCTACATCGGCAGCCCCCGGGCTGTGTCCCTCGCCAGGAGTGCCC
  TCCTGGATGACCACCTACACAGTGAGCCCTACTGGAGCGGGGACCTTCGGGGGAGGAGGAGGAGAGGAAC
  AGGTGGTTCTGAGAGCAGCAAGGCCAATGGGCTCACCGCGGAGAGCAAGGCCTCAGAAGACTTTTTCGGA
  TCTTCCTCAGGCTATTCTTCAGAGGATGACCTTGCAGGCTACACGGACTCAGACCAGCACAGCTCGGGGT
  CCAGGTTAAGGAGTGCAGCATCTCGGGCCGGCTCCTTTGTCTGGACTCTGGTCACTTTTCCAGGCCGCCT
  CTTTGGTCTTCTCTACTGGTGGATTGGCACCACCTGGTACCGCCTGACAACTGCTGCCTCCCTCCTGGAT
  GTCTTCGTCCTAACCAGGTCCAGGCACTTCTCGCTGAACCTGAAGAGTTTTCTGTGGTTCCTTCTGCTCT
  TGCTACTCCTGACTGGTCTGACCTACGGTGCTTGGCATTTTTACCCCTTAGGGCTGCAGACATTGCAACC
  CGCTGTGGTCTCCTGGTGGGCAGCAAAAGAGAGCAGGAAGCAGCCAGAGGTGTGGGAATCCAGAGACGCC
  TCCCAGCACTTCCAGGCTGAGCAGCGCGTTCTCTCCCGGGTTCACTCTCTGGAGCGGCGTCTGGAAGCCC
  TTGCTGCAGACTTTTCCTCCAACTGGCAGAAGGAGGCCATACGGCTGGAACGCCTGGAGCTGCGGCAGGG
  GGCTGCTGGCCATGGAGGAGGCAGTAGCCTGAGCCATGAAGATGCCCTGTCTCTCCTAGAAGGGTTGGTG
  AGCCGCCGCGAGGCTACCCTGAAGGAGGACTTGCGCAGGGACACAGTGGCTCATATCCAGGAAGAATTGG
  CTACCCTGAGGGCAGAGCATCACCAAGACTCGGAAGATCTCTTCAAGAAGATCGTCCAGGCCTCTCAGGA
  GTCCGAAGCCCGAGTCCAGCAGCTGAAGACAGAATGGAAAAGCATGACCCAGGAGGCCTTCCAGGAGAGC
  TCTGTGAAGGAGCTGGGACGGCTGGAAGCCCAGCTGGCCAGCCTGCGGCAGGAGCTGGCTGCCCTGACTC
  TGAAGCAGAACTCGGTGGCAGATGAAGTGGGCCTGCTGCCACAGAAGATCCAGGCTGCCAGGGCTGATGT
  GGAATCCCAGTTCCCTGACTGGATCAGGCAGTTCCTTCTTGGAGACAGGGGTGCGCGCAGCGGGCTCCTG
  CAGAGAGATGAGATGCACGCTCAGCTGCAGGAGCTGGAGAACAAGATCCTTACCAAGATGGCTGAGATGC
  AGGGCAAGTCAGCCAGGGAGGCCGCAGCGTCCCTGGGACAGATACTGCAGAAAGAAGGCATAGTTGGGGT
  GACAGAGGAGCAGGTGCACCGGATCGTCAAGCAGGCCCTGCAGCGCTACAGTGAGGACAGGATTGGAATG
  GTGGATTACGCCCTGGAATCAGGAGGAGCCAGTGTTATCAGCACCCGCTGCTCTGAGACTTATGAGACCA
  AGACGGCACTCCTCAGCCTCTTTGGCATCCCCCTGTGGTACCACTCCCAGTCACCTCGGGTCATTCTGCA
  GCCAGATGTGCACCCAGGCAACTGCTGGGCCTTCCAGGGGCCCCAGGGCTTTGCAGTGGTCCGCCTCTCT
  GCTCGAATCCGACCTACAGCCGTTACCTTAGAGCACGTGCCCAAGGCCTTGTCACCCAACAGCACTATCT
  CCAGTGCTCCCAAGGACTTCGCCATCTTTGGCTTCGATGAAGACCTGCAGCAGGAAGGGACACTTCTTGG
  CACGTTTGCCTACGACCAGGATGGGGAGCCCATCCAGACCTTCTATTTCCAGGCCTCTAAGATGGCCACA
  TACCAAGTTGTGGAGCTTCGGATCCTGACCAACTGGGGCCACCCTGAGTACACGTGTATCTACCGCTTCC
  GGGTGCACGGAGAGCCTGCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR231010 representing NM_001205345
  Red=Cloning site Green=Tags(s)
  
  MSRRSQRLTRYSQDDNDGGSSSSGASSVAGSQGTVFKDSPLRTLKRKSSNMKHLSPAPQLGPSSDSHTSY
  YSESVVRESYIGSPRAVSLARSALLDDHLHSEPYWSGDLRGRRRRGTGGSESSKANGLTAESKASEDFFG
  SSSGYSSEDDLAGYTDSDQHSSGSRLRSAASRAGSFVWTLVTFPGRLFGLLYWWIGTTWYRLTTAASLLD
  VFVLTRSRHFSLNLKSFLWFLLLLLLLTGLTYGAWHFYPLGLQTLQPAVVSWWAAKESRKQPEVWESRDA
  SQHFQAEQRVLSRVHSLERRLEALAADFSSNWQKEAIRLERLELRQGAAGHGGGSSLSHEDALSLLEGLV
  SRREATLKEDLRRDTVAHIQEELATLRAEHHQDSEDLFKKIVQASQESEARVQQLKTEWKSMTQEAFQES
  SVKELGRLEAQLASLRQELAALTLKQNSVADEVGLLPQKIQAARADVESQFPDWIRQFLLGDRGARSGLL
  QRDEMHAQLQELENKILTKMAEMQGKSAREAAASLGQILQKEGIVGVTEEQVHRIVKQALQRYSEDRIGM
  VDYALESGGASVISTRCSETYETKTALLSLFGIPLWYHSQSPRVILQPDVHPGNCWAFQGPQGFAVVRLS
  ARIRPTAVTLEHVPKALSPNSTISSAPKDFAIFGFDEDLQQEGTLLGTFAYDQDGEPIQTFYFQASKMAT
  YQVVELRILTNWGHPEYTCIYRFRVHGEPAH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001205345
• ORF Size: 2193 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001205345.1, NP_001192274.1
• RefSeq Size: 3809 bp
• RefSeq ORF: 2196 bp
• Locus ID: 223697
• UniProt ID: Q8BJS4
• Cytogenetics: 15 E1
• MW: 82.1 kDa
• Gene Summary: As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Required for nuclear migration in retinal photoreceptor progenitors implicating association with cytoplasmic dynein-dynactin and kinesin motor complexes, and probably B-type lamins; SUN1 and SUN2 seem to act redundantly. The SUN1/2:KASH5 LINC complex couples telomeres to microtubules during meiosis; SUN1 and SUN2 seem to act at least partial redundantly. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for Rab5-GDP and participate in the activation of Rab5.[UniProtKB/Swiss-Prot Function]