Anapc5 (NM_001289517) Mouse Tagged ORF Clone

CAT#: MR230972

Anapc5 (myc-DDK-tagged) - Mouse anaphase-promoting complex subunit 5 (Anapc5), transcript variant 3

Product Images

Circular map for MR230972

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Anapc5
• Synonyms: 2510006G12Rik; AA408751; AA536819; AA986414; Anpc5; APC5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230972 representing NM_001289517
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGACCAACGGGGTAGTGCACGCCAACTTGTTTGGCATCAAGGACTGGGTGACGCCCTATAAGATCG
  CCGTCCTGGTGCTGCTCAACGAGATGGGCCGCACGGGCGAGGGCGCCGTCAGCCTCGTGGAGCGGCGGAA
  GCTCAACCAGCTGCTCCTGCCCCTGCTGCAGGGCCCAGATATTACACTGTCAAAGCTGTACAAGCTAATT
  GAAGAATCGTGTCCTCAGCTGGCAAATTCAGTGCAGATCAGAATCAAGCTCATGGCTGAAGGCGAACTGA
  AGGATATGGAACAATTTTTTGATGACCTTTCAGATTCTTTTTCTGGAACTGAACCAGAGGTTCACAAAAC
  GAGTGTAGTAGGTCTGTTCCTGCGCCACATGATCTTGGCCTACAGTAAGCTTTCTTTCAGTCAAGTGTTT
  AAGCTGTACACTGCCCTCCAGCAGTACTTCCAGAACGGGGAGAAAAAGACGGTGGAAGATGCTGATATGG
  ACCGAGAGGATGGAGAGAAACAGATGGAGAAGGAAGAGCTCGACGTGTCCGTGAGAGAAGAGGAAGTATC
  TTGCAGTGGTCCTCTGTCCCAAAAACAAGCGGAATTTTTTCTCTCTCAGCAGGCCGCTTTGTTGAAGAAT
  GATGAGACTAAAGCCCTCACCCCAGCTTCCTTGCAGAAGGAATTGAACAACCTGTTGAAATTTAATCCTG
  ATTTTGCTGAAGCTCATTACCTCAGCTACTTAAACAACCTCCGTGTTCAAGATGTTTTCAGCTCAACACA
  CAGCCTCCTGCATTATTTTGACCGCCTGATTCTCACTGGAGCGGAGGGCAAAAGTAATGGGGAAGAGGGT
  TATGGCCGGAGCCTGAGATACGCTGCTCTCAACCTGGCTGCCCTGCACTGCCGCTTCGGTCACTATCAAC
  AGGCAGAGCTCGCCCTGCAGGAGGCAATTAGGATTGCCCAGGAGTCCAACGATCACGTGTGTCTGCAGCA
  CTGTTTGAGCTGGCTTTATGTGCTGGGGCAGAAGAGAGCCGATAGCTATGTTCTGCTGGAGCACTCTGTG
  AAGAAAGCAGTACATTTTGGGTTACCGAGAGCTTTTGCTGGGAAGACGGCCAACAAACTGATGGATGCCC
  TAAAGGACTCTGACCTCCTGCACTGGAAACACAGCCTGTCAGAACTTATCGATATCAGCATTGCACAGAA
  AACGGCCATCTGGAGGCTGTACGGCCGCAGCACCATGGCACTGCAACAAGCCCAGATGTTGCTGAGCATG
  AACAGCCTGGAGTCGCTGAATGCGGGTGTGCAGCAGAACAATACTGAGTCCTTTGCCGTCGCTCTCTGCC
  ATCTTGCAGAGCTCCATGCAGAACAGGGCTGTTTTGCGGCTGCTGGTGAAGTATTAAAGCACTTGAAGGA
  CCGATTTCCACCCAACAGTCAGCACGCCCAGTTATGGATGCTGTGTGATCAAAAAATACAGTTTGACAGA
  GCAATGAATGATGGCAAATTCCATTTGGCTGATTCACTTGTTACAGGAATCACAGCGCTTAATGGCATAG
  AAGGTGTATACAGGAAAGCAGTCGTACTGCAGGCTCAGAACCAAATGACAGAGGCACACAAGCTACTACA
  GAAGTTGCTGACGTACTGTCAGAAGCTAAAGAACACAGAAATGGTCATCAGTGTCCTCCTATCGGTGGCA
  GAGCTGTACTGGCGATCTTCGTCCCCGACCATCGCCATGCCTGTGCTCCTGGAAGCTCTGGCCCTCTCCA
  AAGAATACCGATTGCAGTACTTGGCCTCCGAAACTGTGCTCAACTTGGCTTATGCCCAGGCCTTAACCCT
  TCTCCACATGGCTATCGAGCCCATCCTAGCCGACGGGGCTGTCCTGGACAAAGGTCGTGCCATGTTCTTA
  GTGTCCAAGTGCCAAGTGGCTTCGGCAGCGTCCTATGACCCAGTGAAGAAAGCGGAAGCTCTGGAAGCTG
  CCATTCAGAACCTCAGTGAAGCCAAGAACTACTTTGCACAAGTCGACTGCAGAGAGCGCATCAGGGATGT
  TGCTTACTTCCAGGCCAGGCTGTACCACGCTCTTGGCAAGACCCAGGAGAGGAACCATTGTGCCATGATC
  TTCCGGCAGCTGCACCAGGAGTTGCCCGCCCATGGGGTGCCCTTGATTAACCACCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230972 representing NM_001289517
  Red=Cloning site Green=Tags(s)
  
  MMTNGVVHANLFGIKDWVTPYKIAVLVLLNEMGRTGEGAVSLVERRKLNQLLLPLLQGPDITLSKLYKLI
  EESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVF
  KLYTALQQYFQNGEKKTVEDADMDREDGEKQMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQAALLKN
  DETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAEGKSNGEEG
  YGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRADSYVLLEHSV
  KKAVHFGLPRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSM
  NSLESLNAGVQQNNTESFAVALCHLAELHAEQGCFAAAGEVLKHLKDRFPPNSQHAQLWMLCDQKIQFDR
  AMNDGKFHLADSLVTGITALNGIEGVYRKAVVLQAQNQMTEAHKLLQKLLTYCQKLKNTEMVISVLLSVA
  ELYWRSSSPTIAMPVLLEALALSKEYRLQYLASETVLNLAYAQALTLLHMAIEPILADGAVLDKGRAMFL
  VSKCQVASAASYDPVKKAEALEAAIQNLSEAKNYFAQVDCRERIRDVAYFQARLYHALGKTQERNHCAMI
  FRQLHQELPAHGVPLINHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001289517
• ORF Size: 2157 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289517.1, NP_001276446.1
• RefSeq Size: 2727 bp
• RefSeq ORF: 2160 bp
• Locus ID: 59008
• Cytogenetics: 5 F
• MW: 81.3 kDa
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]