Apbb1 (NM_001253885) Mouse Tagged ORF Clone

CAT#: MR230951

Apbb1 (myc-DDK-tagged) - Mouse amyloid beta (A4) precursor protein-binding, family B, member 1 (Apbb1), transcript variant 1

Product Images

Circular map for MR230951

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Apbb1
• Synonyms: Fe65; Rir
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230951 representing NM_001253885
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTTCCATCATCCCTGAGCCAGTCGGCCATTAACGCTAACAGCCACGGAGGCCCTGCACTCAGCT
  TCCCCTTACCCCTGCACGCTGCCCATAACCAGCTGCTCAACGCCAAGCTGCAAGCCACAGCTGTGGTACC
  CAAGGACCTTCGAAGTGCTATGGGAGAGGGTAGTGTGCCTGAACCAGGCCCTGCCAATGCCAAGTGGTTA
  AAGGAAGGCCAGAACCAGCTTCGGAGGGCTGCCACAGCCCACCGAGACCAGAACCGAAATGTGACCTTGA
  CCTTGGCGGAGGAGGCCAGCCAGGAGGCTGAGACGGCACCTTTGGGTCCCAAAGGCTTAATGCATCTATA
  CTCTGAGCTGGAGCTCTCGGCCCACAATGCAGCCAACCGAGGGCTTCATGGATCCGCCTTGATCATCAAC
  ACCCAGGAACAGGGACCAGATGAAGGAGAGGAGAAGGCAGCAGGAGAGGCTGAGGAGGATGATGAAGACG
  AAGAGGAGGAGGAGGAGGAGGAGGACTTGTCTTCTCCTCCAGGGCTACCTGAGCCTCTGGAGAATGTGGA
  AGTCCCCTCTGGGCCCCAGGCCCTCACAGACGGCCCCCGGGAACACAGCAAGAGTGCTAGCCTCCTATTT
  GGCATGCGAAACAGTGCAGCCAGTGATGAGGACTCAAGCTGGGCCACCTTATCACAGGGCAGCCCCTCCT
  ATGGCTCCCCGGAGGACACAGATTCCTTCTGGAACCCCAACGCTTTCGAGACGGATTCCGATCTACCGGC
  TGGATGGATGAGGGTACAGGACACCTCAGGGACCTACTACTGGCACATCCCAACAGGGACCACCCAGTGG
  GAACCCCCAGGCCGGGCCTCCCCCTCACAGGGGAGCAGCCCCCAAGAAGAGTCCCAGCTCACCTGGACTG
  GCTTTGCTCACCAAGAAGGCTTTGAGGAAGGAGAGTTTTGGAAGGATGAACCCAGTGAGGAGGCCCCAAT
  GGAGTTGGGACTGAAGGACCCCGAGGAGGCGACATTGTCCTTCCCAGCTCAGAGCCTCAGCCCAGAACCA
  GTTCCCCAGGAGGAAGAGAAGCTGTCCCAACGGAATGCCAACCCAGGGATCAAGTGTTTCGCTGTGCGCT
  CCCTAGGCTGGGTAGAGATGACCGAGGAGGAGCTGGCCCCAGGACGCAGCAGTGTGGCAGTCAACAATTG
  TATCCGCCAGCTCTCCTACCACAAAAACAATCTACATGATCCGATGGCTGGGGGCTGGGGAGAGGGAAAG
  GATCTGCTGCTCCAGCTGGAGGACGAGACTCTAAAGTTGGTGGAGCCACAGAACCAGACGCTGCTGCATG
  CACAGCCCATCGTCAGCATTCGTGTGTGGGGCGTTGGGCGGGACAGTGGAAGAGAGAGGGACTTTGCCTA
  CGTAGCTCGAGATAAGCTGACCCAGATGCTCAAGTGCCACGTGTTTCGCTGTGAGGCACCTGCCAAGAAC
  ATCGCCACCAGCCTGCATGAGATCTGCTCCAAGATCATGTCTGAACGGCGCAATGCTCGCTGCTTGGTCA
  ATGGACTCTCCCTAGACCACTCTAAACTCGTGGATGTCCCTTTCCAAGTGGAATTCCCAGCACCAAAGAA
  TGAGCTGGTGCAGAAGTTCCAAGTCTATTACCTGGGAAATGTGCCAGTTGCTAAACCTGTTGGGGTAGAC
  GTGATTAATGGGGCCCTGGAGTCAGTCCTGTCTTCCAGTAGCCGTGAGCAGTGGACTCCAAGTCACGTCA
  GCGTGGCCCCTGCCACCCTCACCATCTTGCACCAGCAGACAGAAGCGGTGCTGGGGGAGTGCCGGGTGCG
  GTTTCTCTCCTTCCTGGCTGTGGGCAGAGATGTGCACACATTCGCGTTCATCATGGCTGCCGGCCCAGCC
  TCCTTCTGCTGTCACATGTTTTGGTGTGAGCCCAATGCTGCCAGTCTCTCAGAGGCTGTGCAGGCTGCAT
  GCATGCTCCGCTACCAGAAGTGTCTGGATGCTCGCTCCCAGACCTCCACCTCCTGCCTCCCAGCACCCCC
  TGCGGAGTCAGTTGCAAGACGTGTAGGGTGGACAGTCCGCAGGGGTGTTCAGTCGCTGTGGGGTTCCCTC
  AAGCCCAAACGTCTGGGATCCCAGACCCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230951 representing NM_001253885
  Red=Cloning site Green=Tags(s)
  
  MSVPSSLSQSAINANSHGGPALSFPLPLHAAHNQLLNAKLQATAVVPKDLRSAMGEGSVPEPGPANAKWL
  KEGQNQLRRAATAHRDQNRNVTLTLAEEASQEAETAPLGPKGLMHLYSELELSAHNAANRGLHGSALIIN
  TQEQGPDEGEEKAAGEAEEDDEDEEEEEEEEDLSSPPGLPEPLENVEVPSGPQALTDGPREHSKSASLLF
  GMRNSAASDEDSSWATLSQGSPSYGSPEDTDSFWNPNAFETDSDLPAGWMRVQDTSGTYYWHIPTGTTQW
  EPPGRASPSQGSSPQEESQLTWTGFAHQEGFEEGEFWKDEPSEEAPMELGLKDPEEATLSFPAQSLSPEP
  VPQEEEKLSQRNANPGIKCFAVRSLGWVEMTEEELAPGRSSVAVNNCIRQLSYHKNNLHDPMAGGWGEGK
  DLLLQLEDETLKLVEPQNQTLLHAQPIVSIRVWGVGRDSGRERDFAYVARDKLTQMLKCHVFRCEAPAKN
  IATSLHEICSKIMSERRNARCLVNGLSLDHSKLVDVPFQVEFPAPKNELVQKFQVYYLGNVPVAKPVGVD
  VINGALESVLSSSSREQWTPSHVSVAPATLTILHQQTEAVLGECRVRFLSFLAVGRDVHTFAFIMAAGPA
  SFCCHMFWCEPNAASLSEAVQAACMLRYQKCLDARSQTSTSCLPAPPAESVARRVGWTVRRGVQSLWGSL
  KPKRLGSQTP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001253885
• ORF Size: 2130 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001253885.1, NP_001240814.1
• RefSeq Size: 2922 bp
• RefSeq ORF: 2133 bp
• Locus ID: 11785
• UniProt ID: Q9QXJ1
• Cytogenetics: 7 55.9 cM
• MW: 77.9 kDa
• Gene Summary: Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its transcription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).[UniProtKB/Swiss-Prot Function]