Gpsm1 (NM_001199147) Mouse Tagged ORF Clone

CAT#: MR230938

Gpsm1 (myc-DDK-tagged) - Mouse G-protein signalling modulator 1 (AGS3-like, C. elegans) (Gpsm1), transcript variant 3

Product Images

Circular map for MR230938

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gpsm1
• Synonyms: 1810037C22Rik; Ags3; AW107933
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230938 representing NM_001199147
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGCTTCTGCTATGGAGGGCCAGCCTCTGGCCCTGAGCTTGGCAGAGAAGGCTGTGTGCAAGGTGG
  TATATGGTGCCCCTCGCCCTCGCCCATTGCTGCTGCCCGTGGGCCTGGAGCTGTGGCTGTATGTACAGAA
  GATGCGTAACCTCCAGAGAAAGAGGATGGAGGCCTCCTGTCTAGAGCTGGCCCTGGAAGGGGAGCGGCTG
  TGCAAGGCGGGGGACTTCAAGGCAGGTGTGGCTTTCTTCGAGGCTGCTGTGCAGGTGGGCACTGAGGACC
  TGAAGACACTGAGTGCCATCTACAGCCAGCTGGGCAATGCCTACTTCTACCTGAAGGAGTATGCCCGGGC
  CCTGCAGTTCCACAAACATGACTTGCTGCTGGCACGGACCATTGGTGACCGCATGGGGGAGGCCAAGGCT
  AGTGGGAACCTGGGCAACACACTCAAGGTCCTAGGCCGATTCGATGAGGCAATCGTCTGCTGCCAACGAC
  ACTTGGACATTGCCCAGGAGCAGGGGGACAAGGTTGGGGAGGCGAGAGCACTCTACAACATTGGAAATGT
  GTACCACGCCAAGGGCAAACAGCTTTCCTGGAATGCTGCACAGGACCCCGGGCACCTGCCACCTGATGTC
  CGCGAGACACTGCACAGGGCCTCTGAGTTTTATGAGAGGAACCTGTCTTTGGTGAAGGAACTAGGCGACC
  GGGCGGCCCAGGGCAGGGCCTATGGCAACCTGGGTAACACCCACTACCTACTGGGAAACTTCACGGAGGC
  CACAACCTTCCACAAAGAGCGCCTGGCCATCGCCAAGGAGTTTGGGGACAAGGCAGCTGAGCGGAGGGCC
  TACAGCAACCTGGGCAATGCTCACATCTTCTTGGGGCGCTTTGATGTGGCTGCTGAACATTACAAGAAGA
  CGCTGCAGCTGTCTCGGCAGCTCCGGGACCAGGCAGTGGAGGCTCAGGCTTGCTACAGCCTGGGCAACAC
  CTACACACTGCTACAGGACTACGAGCGTGCTGCTGAGTACCACCTGCGGCACCTAGTCATTGCCCAGGAG
  CTGGCTGATAGGGTGGGAGAGGGCCGAGCATGCTGGAGCCTGGGGAACGCCTATGTGTCCATGGGGAGCC
  CTGCACAGGCCTTGACCTTTGCCAAGAAACATCTGCAGATCTCCCAGGAGATTGGAGACCGAAACGGAGA
  ACTGACAGCCCGCATGAATATTGCCCACCTGCAGCTGGCCCTGGGCCGGCTGACTAGCCCAGCAGCAGCG
  GAGAAGCCAGATCTGGCTGGCTATGAGGCACAAGGAGCAAGACCCAAACGGACACAGAGGCTGAGTGCCG
  AGACCTGGGACCTGCTGCGGCTCCCCCTGGACCGGGAGCAGAATGGAGAGACCCACCACACAGGGGACTG
  GAGGGGTCCAGGCAGGGACTCGCTTCCCCTCCCCATGAGGAGCAGGAAGTACCAGGAAGGTCCAGATGCC
  ATTGAGAGGAGACCCCGGGAGGGTAGTCACTCTCCACTGGACAGTGCTGATGTAAGGGTACAGGTGCCTC
  GTACGGGTATTCCCAGGGCCCCGTCTTCTGACGAGGAGTGTTTCTTTGACCTGCTGAGTAAGTTCCAGAG
  CAGTCGCATGGATGACCAGCGCTGTCCCCTGGAGGAAGGCCAGGCTGGGGCTGCTGAGGCCACAGCTGCC
  CCATCCGTGGAGGATAGAGCAGCTCAGTCCTCCGTGACAGCTTCACCACAGACAGAGGAGTTCTTTGACC
  TCATTGCCAGCTCCCAGAGCCGCCGGCTGGACGACCAGAGGGCTAGCGTAGGCAGCCTGCCTGGGCTACG
  CATCACCCTCAACAATGTGGGGCACCTCCGAGGCGACGGGGACGCCCAGGAGCCGGGGGATGAGTTTTTC
  AACATGCTTATCAAATACCAGTCCTCCAGGATTGATGACCAGCGCTGCCCACCCCCTGATGTGCTGCCCC
  GTGGCCCTACCATGCCTGATGAGGATTTCTTCAGCCTTATCCAGAGGGTGCAGGCTAAGCGGATGGATGA
  GCAGCGTGTGGACCTTGCTGGGAGTCCAGAGCAAGAGGCCAGTGGGCTGCCTGATCCCCAGCAGCAGTGT
  CCACCGGGTGCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230938 representing NM_001199147
  Red=Cloning site Green=Tags(s)
  
  MLASAMEGQPLALSLAEKAVCKVVYGAPRPRPLLLPVGLELWLYVQKMRNLQRKRMEASCLELALEGERL
  CKAGDFKAGVAFFEAAVQVGTEDLKTLSAIYSQLGNAYFYLKEYARALQFHKHDLLLARTIGDRMGEAKA
  SGNLGNTLKVLGRFDEAIVCCQRHLDIAQEQGDKVGEARALYNIGNVYHAKGKQLSWNAAQDPGHLPPDV
  RETLHRASEFYERNLSLVKELGDRAAQGRAYGNLGNTHYLLGNFTEATTFHKERLAIAKEFGDKAAERRA
  YSNLGNAHIFLGRFDVAAEHYKKTLQLSRQLRDQAVEAQACYSLGNTYTLLQDYERAAEYHLRHLVIAQE
  LADRVGEGRACWSLGNAYVSMGSPAQALTFAKKHLQISQEIGDRNGELTARMNIAHLQLALGRLTSPAAA
  EKPDLAGYEAQGARPKRTQRLSAETWDLLRLPLDREQNGETHHTGDWRGPGRDSLPLPMRSRKYQEGPDA
  IERRPREGSHSPLDSADVRVQVPRTGIPRAPSSDEECFFDLLSKFQSSRMDDQRCPLEEGQAGAAEATAA
  PSVEDRAAQSSVTASPQTEEFFDLIASSQSRRLDDQRASVGSLPGLRITLNNVGHLRGDGDAQEPGDEFF
  NMLIKYQSSRIDDQRCPPPDVLPRGPTMPDEDFFSLIQRVQAKRMDEQRVDLAGSPEQEASGLPDPQQQC
  PPGAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001199147
• ORF Size: 2115 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001199147.1, NP_001186076.1
• RefSeq Size: 3458 bp
• RefSeq ORF: 2118 bp
• Locus ID: 67839
• UniProt ID: Q6IR34
• Cytogenetics: 2 A3
• MW: 78.6 kDa
• Gene Summary: Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction.[UniProtKB/Swiss-Prot Function]