Cdc27 (NM_001285990) Mouse Tagged ORF Clone

CAT#: MR230917

Cdc27 (myc-DDK-tagged) - Mouse cell division cycle 27 (Cdc27), transcript variant 4

Product Images

Circular map for MR230917

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cdc27
• Synonyms: AI452358; APC3; BC023187
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230917 representing NM_001285990
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACCTTGTCACTGAGTTTGGAGATTCAGCTTGCTTCACTCTTTCCTTGTTGGGACATGTGTATTG
  GTGAGAAGCCAGATCCTGACCAAACATTTAAATTAACATCTCTACAGAATTTTAGCAGTTGTCTTCCCAA
  CACTTGTACAACTCTAGTATCTAATCACAGTTTATCTCACAGACAGCCTGAGACAGTCCTTACAGAAACA
  CCCCAAGACACAATTGAATTAAACAGACTGAATTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAG
  ATTCCTCCGTGTCTTACATTGATTCAACTGTAATTTCACCAGATAACGTCCCCCTTGGACCTGGTACTGC
  CATATTATCTAAACAGGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAACTGCTCTT
  AGCCCATTAACCCCAAGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTAC
  AAAACTACACTAATACACCTTCTGTAATTGATGTGGCACCCACCGGAGCACCTACAAAAAAGACTTTTTG
  TGTTTTACAGTCTGTTGCAAGAATGGGCCAAACTGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGT
  CGAGAGGTCACACCAGTACTTGTTGCACAAACACAAAGTTCTGGCCCACAAACAAGTACAACACCTCAGG
  TATTGAGCCCCACTATCACATCTCCCCCAAACGCATTGCCTCGGAGAAGTTCCCGCCTTTTCACTAGTGA
  CAGTTCTACAACCAAGGAGAATAGCAAAAAGTTAAAAATGAAGTTTCCACCTAAAATCCCTAACAGAAAA
  ACAAAAAGTAAAACTAATAAAGGAGGACTAACTCAGCCCAGCATAAACGATAGTCTGGAAATTACAAAAC
  TGGACTCCTCTATCATTTCAGAAGGGAAAATAACCACAGTCACACCTCAGATCCAGGCATTTAACCTACA
  AAAGGCAGCAGCAGAAGGTTTGATGAGCCTTCTTCGTGAAATGGGAAAAGGTTATTTAGCTTTGTGTTCA
  TACAACTGCAAAGAAGCTATCAATATTTTGAGCCATCTACCTTCTCATCACTACAGTACTGGTTGGGTCC
  TATGCCAGATTGGACGGGCTTATTTTGAACTTTCAGAATACATGCAGGCTGAAAGAATATTCTCAGAGGT
  TAGAAGGATTGAGAGTTTCAGAGTTGAAGGAATGGAGATCTACTCTACAACACTCTGGCATCTTCAGAAA
  GACGTTGCTCTTTCAGTTCTTTCAAAAGATTTAACAGACATGGATAAGAATTCACCAGAGGCCTGGTGTG
  CTGCAGGGAATTGTTTCAGTCTACAACGAGAGCATGATATAGCAATTAAATTCTTCCAAAGAGCTATCCA
  GGTCGATCCAAATTACGCTTACGCCTATACTCTATTAGGACATGAGTTTGTGTTAACTGAAGAACTAGAT
  AAAGCATTAGCATGTTTTCGAAATGCTATAAGAGTAAATCCCAGACATTACAATGCATGGTATGGTTTAG
  GAATGATTTATTACAAGCAAGAGAAGTTCAGCCTCGCAGAAATGCATTTCCAGAAAGCACTTGATATCAA
  CCCTCAGAGTTCAGTTTTACTTTGCCACATTGGAGTAGTTCAGCATGCACTAAAGAAGTCTGAGAAGGCT
  TTGGATACCCTAAACAAAGCCATTGTTATCGATCCCAAGAACCCTCTATGCAAATTTCACAGAGCCTCGG
  TTTTATTTGCAAATGAAAAATACAAGTCTGCTTTACAAGAACTCGAGGAACTGAAGCAGATTGTCCCCAA
  AGAGTCCCTCGTTTACTTCCTAATAGGAAAGGTTTACAAGAAGTTGGGTCAAACTCATCTCGCCTTGATG
  AATTTCTCTTGGGCTATGGATTTAGATCCTAAAGGAGCCAATAATCAGATTAAAGAGGCAATTGACAAGC
  GCTACCTTCCAGATGATGAGGAGCCAATAACCCAGGAGGAACAGATCATGGGGACAGATGAATCCCAGGA
  GAGCAGCATGACAGATGCAGATGACACACAACTTCATGCGGCGGAAAGTGACGAATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230917 representing NM_001285990
  Red=Cloning site Green=Tags(s)
  
  MTTLSLSLEIQLASLFPCWDMCIGEKPDPDQTFKLTSLQNFSSCLPNTCTTLVSNHSLSHRQPETVLTET
  PQDTIELNRLNLESSNSKYSLNTDSSVSYIDSTVISPDNVPLGPGTAILSKQVQNKPKTGRSLLGGPTAL
  SPLTPSFGILPLETPSPGDGSYLQNYTNTPSVIDVAPTGAPTKKTFCVLQSVARMGQTGTKSVFSQSGNS
  REVTPVLVAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRK
  TKSKTNKGGLTQPSINDSLEITKLDSSIISEGKITTVTPQIQAFNLQKAAAEGLMSLLREMGKGYLALCS
  YNCKEAINILSHLPSHHYSTGWVLCQIGRAYFELSEYMQAERIFSEVRRIESFRVEGMEIYSTTLWHLQK
  DVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELD
  KALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKA
  LDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALM
  NFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001285990
• ORF Size: 2088 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285990.1, NP_001272919.1
• RefSeq Size: 5703 bp
• RefSeq ORF: 2091 bp
• Locus ID: 217232
• Cytogenetics: 11 67.79 cM
• MW: 77.8 kDa
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]