Acox1 (NM_001271898) Mouse Tagged ORF Clone

CAT#: MR230813

Acox1 (myc-DDK-tagged) - Mouse acyl-Coenzyme A oxidase 1, palmitoyl (Acox1), transcript variant 2

Product Images

Circular map for MR230813

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Acox1
• Synonyms: A; Acox; AOX; D130055E20Rik; Paox
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230813 representing NM_001271898
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCCGATCTGCGCAAGGAGCGGGCCGCCGCCACCTTCAATCCAGAGTTAATCACGCACATCTTGG
  ATGGTAGTCCGGAGAACACCCGGCGCCGTCGAGAAATCGAGAACTTGATTCTGAACGATCCAGACTTCCA
  ACATGAGGACTATAACTTCCTCACTCGAAGCCAGCGTTACGAGGTGGCTGTTAAGAAGAGTGCCACCATG
  GTGAAGAAGATGAGGGAATTTGGCATCGCAGACCCTGAAGAAATCATGTGGTTTAAAAAACTACATATGG
  TCAATTTTGTGGAACCTGTTGGCCTCAATTACTCCATGTTTATCCCTACCTTGCTGAATCAGGGCACCAC
  TGCTCAGCAGGAGAAATGGATGCACCCGTCCCAAGAACTCCAGATAATTGGCACCTACGCCCAGACGGAG
  ATGGGCCACGGAACTCATCTTCGAGGCTTGGAAACCACTGCCACATATGACCCCAAGACCCAAGAGTTCA
  TTCTCAACAGCCCAACTGTGACTTCCATCAAGTGGTGGCCTGGGGGGCTTGGGAAGACTTCCAATCATGC
  GATAGTCCTGGCTCAGCTCATCACTCGAGGGGAGTGCTACGGGTTACATGCCTTTGTTGTCCCTATCCGT
  GAGATTGGGACCCACAAGCCTCTGCCAGGCATCACTGTTGGGGATATCGGCCCCAAGTTTGGTTATGAAG
  AGATGGATAATGGCTACCTGAAGATGGACAATTACCGTATTCCTAGAGAGAACATGTTGATGAAATATGC
  CCAGGTGAAGCCTGACGGCACGTATGTAAAACCTCTGAGTAACAAGCTGACATATGGGACCATGGTTTTC
  GTAAGGTCCTTCCTCGTGGGAAGTGCAGCTCAGAGTCTGTCCAAGGCATGCACCATTGCCATTCGATACA
  GTGCTGTGAGGCGCCAGTCTGAAATCAAGAGAAGCGAGCCAGAGCCCCAGATTTTGGATTTTCAGACGCA
  GCAGTATAAACTCTTCCCGCTCCTGGCCACCGCCTATGCCTTCCACTTTCTCGGAAGATACATAAAGGAG
  ACCTACATGCGGATTAATGAGAGCATTGGCCAAGGCGACCTGAGTGAGCTGCCTGAGCTTCATGCCCTCA
  CAGCTGGGCTGAAGGCTTTTACTACCTGGACAGCCAATGCTGGTATCGAAGAATGTCGGATGGCTTGCGG
  TGGGCACGGCTATTCTCACAGCAGTGGGATTCCAAATATTTACGTCACGTTTACCCCGGCCTGCACCTTC
  GAGGGGGAGAACACTGTTATGATGCTGCAGACGGCCAGGTTCTTGATGAAAATCTATGACCAGGTTCAGT
  CGGGGAAGCTGGTGGGTGGTATGGTGTCGTACTTGAATGACCTGCCGAGCCAGCGTATCCAGCCGCAGCA
  GGTGGCAGTCTGGCCAACTCTGGTGGACATTAACAGCCTGGACAGCCTGACAGAAGCCTACAAGCTACGT
  GCAGCCAGATTGGTAGAAATTGCTGCAAAAAACCTTCAGGCCCAAGTGAGTCACAGGAAGAGCAAGGAAG
  TGGCGTGGAACTTGACTTCTGTCGACCTTGTTCGCGCAAGTGAGGCGCACTGCCACTACGTGACCGTTAA
  GGTCTTTGCAGATAAACTCCCCAAGATTCAAGACAGAGCCGTGCAAGCCGTGCTGAGGAACCTGTGTCTC
  TTATATTCTCTCTATGGGATCAGCCAGAAAGGAGGGGATTTTCTTGAGGGGAACATCATCACAGGGGCTC
  AGATGTCACAGGTAAACAGTCGGATCCTGGAGCTGCTCACAGTGACTCGCCCCAACGCTGTGGCTTTGGT
  GGATGCCTTTGACTTTAAGGATGTGACCCTTGGCTCTGTTCTCGGCCGCTATGATGGCAATGTGTATGAA
  AACTTGTTTGAGTGGGCCAAGAAGTCCCCACTGAACAAGACAGAGGTCCACGAATCTTACTACAAGCACT
  TGAAGCCCCTGCAGTCGAAGCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230813 representing NM_001271898
  Red=Cloning site Green=Tags(s)
  
  MNPDLRKERAAATFNPELITHILDGSPENTRRRREIENLILNDPDFQHEDYNFLTRSQRYEVAVKKSATM
  VKKMREFGIADPEEIMWFKKLHMVNFVEPVGLNYSMFIPTLLNQGTTAQQEKWMHPSQELQIIGTYAQTE
  MGHGTHLRGLETTATYDPKTQEFILNSPTVTSIKWWPGGLGKTSNHAIVLAQLITRGECYGLHAFVVPIR
  EIGTHKPLPGITVGDIGPKFGYEEMDNGYLKMDNYRIPRENMLMKYAQVKPDGTYVKPLSNKLTYGTMVF
  VRSFLVGSAAQSLSKACTIAIRYSAVRRQSEIKRSEPEPQILDFQTQQYKLFPLLATAYAFHFLGRYIKE
  TYMRINESIGQGDLSELPELHALTAGLKAFTTWTANAGIEECRMACGGHGYSHSSGIPNIYVTFTPACTF
  EGENTVMMLQTARFLMKIYDQVQSGKLVGGMVSYLNDLPSQRIQPQQVAVWPTLVDINSLDSLTEAYKLR
  AARLVEIAAKNLQAQVSHRKSKEVAWNLTSVDLVRASEAHCHYVTVKVFADKLPKIQDRAVQAVLRNLCL
  LYSLYGISQKGGDFLEGNIITGAQMSQVNSRILELLTVTRPNAVALVDAFDFKDVTLGSVLGRYDGNVYE
  NLFEWAKKSPLNKTEVHESYYKHLKPLQSKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001271898
• ORF Size: 1983 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001271898.1, NP_001258827.1
• RefSeq Size: 3992 bp
• RefSeq ORF: 1986 bp
• Locus ID: 11430
• UniProt ID: Q9R0H0
• Cytogenetics: 11 E2
• MW: 75.1 kDa
• Gene Summary: This gene encodes a member of the acyl-coenzyme A oxidase family. The encoded protein is localized to peroxisomes and is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-coenzyme A to 2-trans-enoyl-coenzyme A. Disruption of this gene results in microvesicular steatohepatitis, spontaneous peroxisome proliferation, and the eventual development of hepatocellular carcinomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]