Lcp1 (NM_001247984) Mouse Tagged ORF Clone

CAT#: MR230727

Lcp1 (myc-DDK-tagged) - Mouse lymphocyte cytosolic protein 1 (Lcp1), transcript variant 1

Product Images

Circular map for MR230727

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Lcp1
• Synonyms: AW536232; D14Ertd310e; LCP-1; Pls2; pp65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230727 representing NM_001247984
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGAGGATCCGTGTCTGACGAAGAAATGATGGAGCTCAGAGAGGCTTTTGCCAAAGTTGATACCG
  ATGGCAACGGATACATCAGCTGCAATGAGCTAAATGACTTGTTCAAGGCCGCCTGCCTGCCTCTGCCTGG
  GTACCGAGTGAGAGAAATCACAGAAAACCTGATGGCCACAGGTGATCTGGACCAAGATGGAAAGATCAGC
  TTTGATGAGTTTATCAAGGTCTTCCATGGCTTAAAAAGCACCGAGGTTGCCAAAACCTTCCGAAAAGCTA
  TCAACAAGAAGGAAGGGATCTGTGCGATTGGCGGCACCTCTGAGCAGTCCAGCGTTGGTACCCAGCACTC
  TTACTCAGAGGAAGAAAAGTATGCCTTTGTCAACTGGATAAACAAAGCCCTGGAGAATGACCCCGACTGC
  CGGCATGTCATCCCCATGAACCCCAACACCGACGATCTCTTCAATGCTGTAGGCGATGGCATAGTTCTTT
  GTAAAATGATCAACCTGTCTGTGCCAGACACGATTGACGAGAGAACGATCAACAAGAAAAAGCTCACACC
  ATTCACCATTCAGGAAAACTTGAACTTGGCTCTGAACTCTGCCTCTGCCATTGGGTGCCACGTGGTTAAT
  ATAGGGGCCGAGGACCTGAAGGAGGGCAAGCCTTACCTGGTCCTGGGACTTTTGTGGCAAGTCATCAAGA
  TTGGGTTGTTTGCTGACATTGAACTCAGCAGAAATGAAGCTCTGATTGCTCTTTTGAGAGAAGGAGAGAG
  CCTAGAGGATTTGATGAAGTTGTCTCCTGAAGAACTCCTGCTGCGGTGGGCTAACTACCACCTAGAAAAC
  GCAGGCTGCACCAAAATCACCAACTTCAGCACCGACATCAAGGACTCCAAAGCTTATTACCACCTGCTCG
  AGCAAGTGGCTCCAAAAGGAGATGAAGAAGGGATCCCGGCGGTTGTGATTGACATGTCAGGACTGAGGGA
  GAAGGATGACATCCAGAGGGCAGAGTGCATGCTGCAACAGGCGGAGAGGCTGGGCTGCCGGCAGTTTGTC
  ACAGCTACTGATGTTGTCCGAGGGAACCCCAAGTTGAACCTGGCCTTCATTGCCAACCTCTTCAACAAAT
  ACCCTGCCTTACACAAACCAGAGAACCAGGACATTGACTGGGGGGCTCTCGAAGGTGAGACGAGGGAAGA
  GCGGACCTTCAGGAATTGGATGAACTCCCTGGGCGTTAACCCGCGCGTCAATCACTTGTACAGCGACTTA
  TCGGATGCCTTAGTCATCTTCCAGCTCTATGAGAAGATCAAAGTCCCTGTTGATTGGAACAGAGTAAACA
  AGCCTCCATACCCCAAGCTGGGGGGCAATATGAAAAAGCTGGAGAACTGTAATTATGCAGTGGACCTGGG
  GAAGAATCAAGCTAAATTCTCCCTGGTTGGCATCGCAGGACAAGACCTCAATGAAGGAAACCGAACTCTC
  ACGCTGGCATTGGTTTGGCAGCTCATGAGAAGGTACACACTGAATATCCTGGAAGATATCGGAGGTGGAC
  AGAAGGTCAATGATGACATTATTGTCAACTGGGTGAATACGACCTTGAAGGAGGCACAGAAAAGCTCATC
  CATTGCTAGCTTCAAGGACCCAAAGATCAGTACCAGCCTCCCGGTTCTGGATCTCATTGACGCCATTCAG
  CCAGGTTCCATAAACTATGACCTTCTAAAGACAGAAAACCTGGATGATGAAGAGAAACTCAACAATGCAA
  AGTATGCCATCTCTATGGCCAGAAAAATCGGAGCAAGGGTGTACGCCCTCCCAGAAGACCTGGTTGAAGT
  GAACCCCAAAATGGTCATGACAGTGTTTGCCTGCCTCATGGGGAAAGGGATGAAGAGGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230727 representing NM_001247984
  Red=Cloning site Green=Tags(s)
  
  MARGSVSDEEMMELREAFAKVDTDGNGYISCNELNDLFKAACLPLPGYRVREITENLMATGDLDQDGKIS
  FDEFIKVFHGLKSTEVAKTFRKAINKKEGICAIGGTSEQSSVGTQHSYSEEEKYAFVNWINKALENDPDC
  RHVIPMNPNTDDLFNAVGDGIVLCKMINLSVPDTIDERTINKKKLTPFTIQENLNLALNSASAIGCHVVN
  IGAEDLKEGKPYLVLGLLWQVIKIGLFADIELSRNEALIALLREGESLEDLMKLSPEELLLRWANYHLEN
  AGCTKITNFSTDIKDSKAYYHLLEQVAPKGDEEGIPAVVIDMSGLREKDDIQRAECMLQQAERLGCRQFV
  TATDVVRGNPKLNLAFIANLFNKYPALHKPENQDIDWGALEGETREERTFRNWMNSLGVNPRVNHLYSDL
  SDALVIFQLYEKIKVPVDWNRVNKPPYPKLGGNMKKLENCNYAVDLGKNQAKFSLVGIAGQDLNEGNRTL
  TLALVWQLMRRYTLNILEDIGGGQKVNDDIIVNWVNTTLKEAQKSSSIASFKDPKISTSLPVLDLIDAIQ
  PGSINYDLLKTENLDDEEKLNNAKYAISMARKIGARVYALPEDLVEVNPKMVMTVFACLMGKGMKRV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001247984
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001247984.1, NP_001234913.1
• RefSeq Size: 4007 bp
• RefSeq ORF: 1884 bp
• Locus ID: 18826
• UniProt ID: Q61233
• Cytogenetics: 14 39.63 cM
• MW: 70.6 kDa
• Gene Summary: Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.[UniProtKB/Swiss-Prot Function]