Repin1 (NM_001079903) Mouse Tagged ORF Clone

CAT#: MR230639

Repin1 (myc-DDK-tagged) - Mouse replication initiator 1 (Repin1), transcript variant 3

Product Images

Circular map for MR230639

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Repin1
• Synonyms: AI425994; Ap4; E430037F08Rik; Zfp-464; Zfp464
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230639 representing NM_001079903
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGGGCGCTGCGTCCCTGGAGGGCACGCGGGAGAGTTTTCTCTGTCATCCGGGGGCTACCGAAGTG
  TGGGCCGAAGCAGCCGAAGTCTCCCCAGGAACATCCCCAGAAGGAGCTGGAAAAAGCCTCACCCCCAGCT
  CTTCAGTCTTCAGGCAGAGGAAGAACCGATGCTGGAACAGCGCTGCAGGGGCCCCACGGCCATGGGCCCA
  GCTCAGCCCTGGCTCTTTTCTGGGCCCTCCCAGGAGTCCTCCCAGCCCGACAGAGGGTTGAGGTACCAGG
  GCAAATCAGCTCAGCCAAGAGGCCAAACCCCAGGCAAGGTCCATCGTTGTGCCCACTGTCGGAAGCGCTT
  CCCGGGCTGGGTGGCCCTGTGGCTTCATGCTCGGCGGTGCCAGGCCCGGCTGCCTCTGCCCTGCCATGAA
  TGCAACCAGCGCTTTCGCCACGCCCCCTTCTTAGCGCTGCATCTTCAGGTTCATGCTTCTGCAGTCCCCG
  ACCTGGGTTTCATCTGCCACCTATGTGGGCATAGCTTCCGAGGATGGGTAGCCCTGGTTCTGCATCTGCG
  GGCTCACTCAGCTTCAAAGCGGCCCATCACTTGCCCTGAATGCGACAGACGCTTCTGGCGACAAAAACAG
  CTTCGAGCTCATCTGCGAAGGTGCCAGCCCCCTGTCCCTGAGGCCCGGCCCTTCATATGCGGCAACTGTG
  GCCGGAGCTTTGCCCAATGGGACCAACTGGTTGTTCACAAGCGGGTGCACGTGGCTGAGGCCTTGGAGGA
  GGCAGCAGCCAAAGCCCTGGGTCCTCGCCCACGAGGACGTCCCGCAGCTCCCAGGCCTGGTGGAGACGCT
  GTGGACCGCCCCTTCCAGTGTGCCTGCTGCGGCAAGCGTTTCCGCCACAAGCCCAATCTGATCGCCCACC
  GCCGCGTGCACACTGGTGAGCGACCACACCAGTGCCCAGAGTGCGGGAAGCGTTTCACCAACAAGCCCTA
  CCTGACCTCGCACCGGCGCATACATACCGGCGAGAAGCCCTACCCATGCACCGAGTGTGGCCGCCGCTTC
  CGCCACAAACCCAACCTGTTGTCGCACAGCAAAATCCACAAGCGCTTGGAGGTCTCAGCGCAGGCTGCCC
  CACACCCCGAGAGTCACCAGATTGCAGCAGAGCCTATGGCACAACCTGCACTTGGGGTGCCCCTGGGGTC
  CCCGCGGACCCCAGCTGAGGCACCTGCGCTCCTGCATAGCTGCTCCGACTGCGGCCGCAGCTTCCGGCTG
  GAGCGCTTCCTGCGGCTACACCAGAGGCAGCACACAGGGGAGAGGCCCTTCGCCTGCACAGAGTGTGGCA
  AGAACTTCGGCAAGAAGACGCACCTGGTGGCGCACTCACGCGTGCACTCCGGCGAACGTCCCTTCGCCTG
  CGAGGAGTGTGGTCGCCGTTTCTCACAGGGCAGCCACCTGGCAGCCCACCGGCGAGACCATGCACCAGAG
  AGGCCCTTCGTGTGCCCGGACTGCGGCAAGGCTTTCCGCCACAAGCCCTACCTGGCTGCGCACCGACGCA
  TCCACACAGGCGAGAAACCCTATGTGTGTCCCGACTGTGGCAAAGCTTTCAGTCAGAAGTCCAACCTGGT
  GTCCCACCGGCGCATCCACACAGGCGAGCGGCCCTACGCCTGCCCCGACTGTGATCGTAGCTTCAGTCAG
  AAGTCCAATCTTATCACACACCGGAAGAGCCACATCCGGGATGGCGCCTTCTGTTGTGCCATCTGTGGCC
  AGACCTTTGATGACGAGGACCGACTCTTGATGCACCAGAAGAAGCATGATGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230639 representing NM_001079903
  Red=Cloning site Green=Tags(s)
  
  MDGRCVPGGHAGEFSLSSGGYRSVGRSSRSLPRNIPRRSWKKPHPQLFSLQAEEEPMLEQRCRGPTAMGP
  AQPWLFSGPSQESSQPDRGLRYQGKSAQPRGQTPGKVHRCAHCRKRFPGWVALWLHARRCQARLPLPCHE
  CNQRFRHAPFLALHLQVHASAVPDLGFICHLCGHSFRGWVALVLHLRAHSASKRPITCPECDRRFWRQKQ
  LRAHLRRCQPPVPEARPFICGNCGRSFAQWDQLVVHKRVHVAEALEEAAAKALGPRPRGRPAAPRPGGDA
  VDRPFQCACCGKRFRHKPNLIAHRRVHTGERPHQCPECGKRFTNKPYLTSHRRIHTGEKPYPCTECGRRF
  RHKPNLLSHSKIHKRLEVSAQAAPHPESHQIAAEPMAQPALGVPLGSPRTPAEAPALLHSCSDCGRSFRL
  ERFLRLHQRQHTGERPFACTECGKNFGKKTHLVAHSRVHSGERPFACEECGRRFSQGSHLAAHRRDHAPE
  RPFVCPDCGKAFRHKPYLAAHRRIHTGEKPYVCPDCGKAFSQKSNLVSHRRIHTGERPYACPDCDRSFSQ
  KSNLITHRKSHIRDGAFCCAICGQTFDDEDRLLMHQKKHDA
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079903
• ORF Size: 1803 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079903.1, NP_001073372.2
• RefSeq Size: 3106 bp
• RefSeq ORF: 1806 bp
• Locus ID: 58887
• UniProt ID: Q5U4E2
• Cytogenetics: 6 B2.3
• MW: 68.5 kDa
• Gene Summary: Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending (By similarity).[UniProtKB/Swiss-Prot Function]