Zscan21 (NM_011757) Mouse Tagged ORF Clone

CAT#: MR230495

Zscan21 (myc-DDK-tagged) - Mouse zinc finger and SCAN domain containing 21 (Zscan21), transcript variant 1

Product Images

Circular map for MR230495

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Zscan21
• Synonyms: AI326272; CTfin51; RU49; Zfp-38; Zfp38; Zipro1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230495 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAAGGTGGTGGGCATGGCCACAGTTCTGGGCCCCAGGCCACCTCAGGAATCTATGGGACCTTCGC
  CCATTAAAGTAGAAGAGGATGAAGAGAAAGACAAGTGCTGCCCTACCCTAGAGCTATCCCATAAGCACTT
  CAGACAGTCTGGGAACCAAGATACCCTTGAGCCAATGGGTCCTTCGACCATTAAAGCTGAAGAGGATGAA
  AGCAAAGACAAGTGCCGCCCTAACCTAGAGATATCCCGTAAGAGCTTCAAGCAGTTTGGGTACCAAGACA
  CTCTTGAGCAGTTGGGACCTTCGACCATTAAAGCTGAAGAGGATGATGAGAAGGATAAGGGCCACCCTAG
  CCCAGAGATATCTCGACAGCGCTTCAGGCAATTTGGGTACCATGACACTCCTGGGCCCCGAGAGGCACTG
  AGCCAGCTTCGGGTGCTCTGCTGTGAGTGGCTACAGCCCGAGATCCACACCAAAGAGCAGATTCTAGAGC
  TACTGGTTCTGGAGCAGTTCCTGACCATCCTGCCCCGAGAGCTCCAGACCTGGGTACAGCAGCACTGCCC
  TGAAAGTGCAGAGGAGGCCGTCACTCTCCTGGAAGACCTGGAGCAAGAACTTGATGAGCCAGGACTGCAG
  GTCTCATCTCCTCCAAATGAACAGAAGCAGTCTTGGGAGAAAATGTCAACTTCAGGAACGGCAATGGAGT
  CCTTAAGCAGTACTGAGACCCAACATGTGGATGCCAGCCCTAAATATGAGTTTTGGGGGCCCCTGTACAT
  CCAAGAGACTGGTGAGGAGGAGGTTTTTACTCAGGATCCAAGAAAGCGCCAAGGTTTTAAATCGAATCCC
  CAGAAGGAGGACTCAGCAGATGAGCACAGAAGTTCTGAAGAAGAGTCTCATGCAGACGGACTCAAAAGAA
  CCGTCATCCCCATGATTCCTGCCAATAAGTATGGGTCACGGTCAGAAAGGCAGTGGGCCAACAACCTGGA
  GAGGGAGAGAGGGACAAAAGCCTCTCTTCAAGACACAGGTTCCAGGAAAGGGGCAGAGCCAGCGTCTACT
  AGGCCTGCCCCAGGAGAGAAACGTTACATATGTGCAGAGTGTGGGAAGGCCTTTAGCAATAGCTCAAACC
  TCACTAAGCACCGGAGAACACACACTGGGGAGAAGCCTTATGTGTGTACAAAGTGTGGGAAGGCTTTCAG
  CCACAGCTCCAACCTTACCCTTCATTACCGAACTCACCTGGTGGACCGGCCCTATGACTGTAAGTGTGGG
  AAAGCCTTTGGGCAGAGCTCAGACCTCCTTAAACATCAAAGGATGCACACGGAAGAGGCGCCCTATCAGT
  GTAAAGACTGTGGGAAAGCCTTTAGTGGGAAGGGCAGCCTCATTCGACACTATCGCATCCACACAGGGGA
  GAAGCCCTATCAGTGTAATGAGTGTGGAAAGAGTTTTAGTCAGCATGCAGGTCTCAGTTCTCATCAGCGT
  CTGCATACAGGGGAGAAACCCTATAAGTGTAAGGAGTGTGGCAAAGCCTTCAACCATAGTTCAAATTTTA
  ATAAGCATCATAGAATCCATACTGGCGAAAAGCCCTATTGGTGTAGCCACTGTGGGAAAACCTTCTGTAG
  CAAGTCCAATCTGTCCAAGCATCAGAGAGTTCACACTGGAGAGGGAGAAGTCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230495 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTKVVGMATVLGPRPPQESMGPSPIKVEEDEEKDKCCPTLELSHKHFRQSGNQDTLEPMGPSTIKAEEDE
  SKDKCRPNLEISRKSFKQFGYQDTLEQLGPSTIKAEEDDEKDKGHPSPEISRQRFRQFGYHDTPGPREAL
  SQLRVLCCEWLQPEIHTKEQILELLVLEQFLTILPRELQTWVQQHCPESAEEAVTLLEDLEQELDEPGLQ
  VSSPPNEQKQSWEKMSTSGTAMESLSSTETQHVDASPKYEFWGPLYIQETGEEEVFTQDPRKRQGFKSNP
  QKEDSADEHRSSEEESHADGLKRTVIPMIPANKYGSRSERQWANNLERERGTKASLQDTGSRKGAEPAST
  RPAPGEKRYICAECGKAFSNSSNLTKHRRTHTGEKPYVCTKCGKAFSHSSNLTLHYRTHLVDRPYDCKCG
  KAFGQSSDLLKHQRMHTEEAPYQCKDCGKAFSGKGSLIRHYRIHTGEKPYQCNECGKSFSQHAGLSSHQR
  LHTGEKPYKCKECGKAFNHSSNFNKHHRIHTGEKPYWCSHCGKTFCSKSNLSKHQRVHTGEGEVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011757
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011757.3, NP_035887.2
• RefSeq Size: 2321 bp
• RefSeq ORF: 1668 bp
• Locus ID: 22697
• UniProt ID: Q07231
• Cytogenetics: 5 76.96 cM
• MW: 63 kDa
• Gene Summary: Strong transcriptional activator. Associated with meiosis in both male and female gametogenesis. May have different functions in somatic cells.[UniProtKB/Swiss-Prot Function]