Slc33a1 (NM_001272035) Mouse Tagged ORF Clone

CAT#: MR230472

Slc33a1 (myc-DDK-tagged) - Mouse solute carrier family 33 (acetyl-CoA transporter), member 1 (Slc33a1), transcript variant 2

Product Images

Circular map for MR230472

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Slc33a1
• Synonyms: Acatn; AI315656; AI788741; D630022N01Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230472 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACCGACCATCTCCCACAAGGACAGCAGTCGGCAGCGGCGGTCAGGCATGTTTAGCCACGCTCTGG
  ATATGAAGAGTGGCCCGCTGCCACCGGGCGGCTGGGATGACAGTCGCCGAGACTCGGTGGGCGGGGAGGG
  GGACCGGGAAGTTCTTCTAGGTGATGCCGGCCCTGGTGACTTACCTAAAGCCCCGCGGAGCTATAGATCC
  GAACTAAGTAGCATTTTGTTACTACTCTTTCTTTACGTGCTTCAGGGCATTCCCTTAGGCCTGGCGGGGA
  GCATCCCCCTCATTTTGCAAAGCAAAAATGTTAGCTATACAGATCAGGCTTTCTTCAGTTTTGTCTTCTG
  GCCATTCAGTCTTAAGTTGCTCTGGGCTCCCTTGGTTGATGCCGTCTACTTTAAGAACTTTGGTCGTCGC
  AAGTCCTGGCTTGTCCCTACTCAGTATACACTAGGAATCTTCATGATATATTTATCCACTCAAGTGGACC
  GTTTGCTCGGAAATATTGATGGCAGAACACCGGATGTAGTTGCTCTCACTGTGACATTCTTCTTGTTTGA
  ATTCCTGGCTGCCACACAGGACATCGCTGTGGATGGTTGGGCATTAACTATGTTATCCCGGGAAAACGTG
  GGCTATGCTTCGACATGCAATTCTGTGGGCCAAACAGCTGGCTACTTTTTGGGCAATGTTTTATTTTTGG
  CTCTTGAATCTGCTGACTTTTGTAACAAATACTTGCGGTTTCAGCCTCAACCCAGGGGAATCGTTACCCT
  TTCAGATTTTCTATTTTTCTGGGGAACTGTATTTTTAATAACAACAACATTAGTAGCCCTTCTGAAAAAA
  GAAAACAGAGAAGCGTCAATAGTAAAAGAAGAGACCCAAGGGATCACAGACACTTACAAGCTGCTATTCT
  CAATTATAAAAATGCCAGCAGTTCTGGCCTTTTGCCTTCTGATTCTAACGTCAAAGATTGGCTTCTCAGC
  AGCTGATGCTGTGACAGGCCTGAAGCTGGTGGAAGAAGGGGTGCCTAAAGAGCACCTGGCCTTACTAGCT
  GTCCCAATGGTCCCTCTGCAGATAATCCTGCCACTCCTCATCAGCAAGTATACTGCAGGTCCCCAGCCTC
  TGAACATATTTTACAAAGCCATGCCCTACAGATTATTGCTTGGATTAGAATATGCTCTACTTGTTTGGTG
  GACTCCTAAAGTAGAGCATCAAGGAGGATTCCCTCTATATTACTATATTATAGTGCTGCTGAGTTATGCA
  TTACATCAGGTCACTCTGTACAGCATGTATGTGTCGATAATGGCTTTCAATGCCAAGGTCAGTGATCCTC
  TTATTGGGGGAACATACATGACCCTTTTAAACACTGTGTCTAATCTGGGAGGAAACTGGCCTTCTACAGT
  GGCTCTTTGGCTAGTGGATCCCCTCACAGTGAAGGAATGTGTAGGAGCTTCAAACCAGAATTGTCGGACA
  CCTGATGCTATCGAACTTTGCAAAAAACTTGGAGGCTCGTGTGTTACAGCTCTGGATGGTTACTATGTGG
  AATCCATTATCTGTGTTCTCATTGGATTTGGTTGGTGGTTCTTTCTTGGTCCAAAATTTAAAAAGTTACA
  GGATGAAGGACCATCCTCATGGAAGTGCAAAAGGAACAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230472 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSPTISHKDSSRQRRSGMFSHALDMKSGPLPPGGWDDSRRDSVGGEGDREVLLGDAGPGDLPKAPRSYRS
  ELSSILLLLFLYVLQGIPLGLAGSIPLILQSKNVSYTDQAFFSFVFWPFSLKLLWAPLVDAVYFKNFGRR
  KSWLVPTQYTLGIFMIYLSTQVDRLLGNIDGRTPDVVALTVTFFLFEFLAATQDIAVDGWALTMLSRENV
  GYASTCNSVGQTAGYFLGNVLFLALESADFCNKYLRFQPQPRGIVTLSDFLFFWGTVFLITTTLVALLKK
  ENREASIVKEETQGITDTYKLLFSIIKMPAVLAFCLLILTSKIGFSAADAVTGLKLVEEGVPKEHLALLA
  VPMVPLQIILPLLISKYTAGPQPLNIFYKAMPYRLLLGLEYALLVWWTPKVEHQGGFPLYYYIIVLLSYA
  LHQVTLYSMYVSIMAFNAKVSDPLIGGTYMTLLNTVSNLGGNWPSTVALWLVDPLTVKECVGASNQNCRT
  PDAIELCKKLGGSCVTALDGYYVESIICVLIGFGWWFFLGPKFKKLQDEGPSSWKCKRNN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001272035
• ORF Size: 1653 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001272035.1, NP_001258964.1
• RefSeq Size: 2430 bp
• RefSeq ORF: 1653 bp
• Locus ID: 11416
• UniProt ID: Q99J27
• Cytogenetics: 3 E1
• MW: 61.1 kDa
• Gene Summary: Probable acetyl-CoA transporter necessary for O-acetylation of gangliosides (PubMed:10570973). Negatively regulates BMP signaling (By similarity).[UniProtKB/Swiss-Prot Function]