Grk6 (NM_001286065) Mouse Tagged ORF Clone

CAT#: MR230449

Grk6 (myc-DDK-tagged) - Mouse G protein-coupled receptor kinase 6 (Grk6), transcript variant 6

Product Images

Circular map for MR230449

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Grk6
• Synonyms: Gprk6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230449 representing NM_001286065
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCGAGAACATCGTAGCGAACACGGTGCTACTCAAGGCCCGGGAAGGTGGTGGCGGGAATCGCA
  AAGGCAAGAGCAAGAAATGGCGCCAGATGCTGCAGTTCCCCCATATCAGCCAGTGTGAGGAGCTTCGACT
  CAGCCTTGAGCGTGACTACCACAGCCTATGTGAGCGCCAGCCCATTGGGCGCCTGTTATTTCGTGAGTTC
  TGTGCTACGAGACCTGAGCTGACCCGGTGTACTGCCTTCCTGGATGGGGTGTCTGAATATGAGGTGACCC
  CTGATGAGAAGCGGAAAGCATGTGGGCGCCGACTAATGCAGAACTTTCTGAGCCACACGGGTCCTGACCT
  CATCCCTGAAGTTCCACGGCAGCTGGTGAGTAACTGTGCCCAGCGGCTAGAGCAGGGACCCTGCAAAGAC
  CTCTTCCAGGAGCTGACCCGGCTGACCCACGAGTACCTGAGCACGGCCCCTTTTGCCGACTACCTCGACA
  GCATCTACTTCAACCGTTTTCTGCAGTGGAAGTGGCTGGAAAGGCAACCAGTGACCAAAAACACCTTCAG
  GCAGTACCGAGTCCTGGGCAAAGGTGGCTTTGGGGAGGTATGTGCCTGCCAGGTGCGGGCAACAGGCAAG
  ATGTACGCATGCAAGAAACTGGAAAAGAAGCGGATAAAGAAGCGAAAGGGGGAGGCCATGGCTCTCAACG
  AGAAACAGATCTTGGAGAAAGTGAACAGTAGGTTTGTAGCTGGCTTTCCTGAAGCACGTGCTGTCTTCTA
  TGCTGCTGAGATCTGCTGTGGCCTGGAAGACCTGCACCGGGAACGCATTGTGTACAGGGATCTAAAGCCA
  GAGAATATCCTTCTGGATGACCATGGCCACATTCGGATCTCGGACCTGGGACTGGCCGTGCATGTGCCTG
  AGGGCCAGACCATCAAAGGCCGTGTGGGCACTGTGGGCTACATGGCTCCAGAGGTGGTGAGGAATGAGCG
  CTACACGTTCAGTCCTGACTGGTGGGCGCTAGGCTGCCTCCTGTACGAGATGATCGCAGGTCAGTCGCCC
  TTCCAGCAGAGGAAGAAGAAGATAAAGCGCGAAGAGGTGGAGCGGCTGGTCAAGGAAGTGGCCGAGGAGT
  ACACAGACCGCTTTTCCTCACAGGCGCGCTCACTCTGTTCTCAGCTTCTTAGCAAGGACCCTGCTGAGCG
  CCTGGGGTGTCGTGGAGGTGGCGCCCGTGAGGTAAAGGAGCACCCCCTTTTCAAGAAACTGAATTTCAAG
  CGGCTGGGAGCTGGCATGCTAGAGCCACCTTTTAAGCCTGATCCCCAGGCTATTTATTGCAAGGATGTCC
  TGGACATTGAACAGTTCTCTACAGTTAAAGGTGTGGATCTGGAGCCCACAGACCAAGACTTCTACCAGAA
  GTTTGCCACAGGCAGTGTGTCCATCCCCTGGCAGAATGAGATGGTGGAGACCGAGTGCTTCCAGGAACTC
  AATGTCTTTGGGCTGGATGGGTCTGTTCCCCCAGACCTGGACTGGAAGGGCCAGCCCACTGCACCCCCCA
  AGAAGGGATTGCTACAGAGACTCTTCAGTCGCCAAGATTGCTGTGGGAACTGCAGCGACAGTGAGGAAGA
  GCTCCCCACCCGCCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230449 representing NM_001286065
  Red=Cloning site Green=Tags(s)
  
  MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREF
  CATRPELTRCTAFLDGVSEYEVTPDEKRKACGRRLMQNFLSHTGPDLIPEVPRQLVSNCAQRLEQGPCKD
  LFQELTRLTHEYLSTAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGK
  MYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKP
  ENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVRNERYTFSPDWWALGCLLYEMIAGQSP
  FQQRKKKIKREEVERLVKEVAEEYTDRFSSQARSLCSQLLSKDPAERLGCRGGGAREVKEHPLFKKLNFK
  RLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVDLEPTDQDFYQKFATGSVSIPWQNEMVETECFQEL
  NVFGLDGSVPPDLDWKGQPTAPPKKGLLQRLFSRQDCCGNCSDSEEELPTRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001286065
• ORF Size: 1626 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286065.1, NP_001272994.1
• RefSeq Size: 2912 bp
• RefSeq ORF: 1629 bp
• Locus ID: 26385
• UniProt ID: O70293
• Cytogenetics: 13 30.06 cM
• MW: 62.6 kDa
• Gene Summary: Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]