Tjap1 (NM_001252474) Mouse Tagged ORF Clone

CAT#: MR230438

Tjap1 (myc-DDK-tagged) - Mouse tight junction associated protein 1 (Tjap1), transcript variant 3

Product Images

Circular map for MR230438

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tjap1
• Synonyms: 0610041D19Rik; AI415281; AW121008; Pilt; Tjp4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230438 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGTGCTGCCCCTGCTAAGAAACCTTACCGTAAGGCGCCTCCAGAGCACCGAGAGCTACGCCTGG
  AGATTCCCGTGTCCCGGCTCGAGCAAGAGGAGTCCCTGACTGACGCAGAGCGGATGAAACTTTTGCAGCA
  GGAGAATGAAGAACTTCGCAAACGCCTGGCCTCAGCTACCAGACGCACTGAGGCCTTGGAGCGGGAGCTG
  GAAATAGGACAAGACTGCCTAGAACTGGAGTTAGGACAGAGCCGTGAGGAGCTGGACAAGTTTAAGGACA
  AATTCCGCAGGCTACAGAACAGCTACACGGCATCCCAGCGGACCAACCAGGAACTGGAGGACAAGCTGCA
  CGCACTGATCAAGAAGGCTGAGATGGACAGGAAGACGCTGGACTGGGAGATTGTGGAGCTGACCAACAAG
  CTACTGGATGCCAGGAACACCATCAATAAACTGGAAGAGCTCAATGAGCGGTACCGGCTGGACTGCAACC
  TGGCGGTGCAGCTTCTCAAGTGCAACAAGTCCCACTTCCGGAACCACAAGCTTGCTGACCTACCCTGTGA
  GCTCCAGGACATGGTTCGAAAACATCTGCGCAGTGGGCAGGAGGTTGCCAGCCCCAGCCCCAGCCCTTCT
  TCCAGCCTGTCCCCAGGGGCTGTGGTGCCTACCTCAGTCATTGCCCGGGTGTTGGAGAAACCTGAGTCTC
  TCTTGCTCAATTCAGCTCAGTCTGGCAGTGCTGGGCGCCCCTTGGCTGAGGATGTATTCGTGCATGTGGA
  CATGAGTGGGGGTGACCCAGCCAGCCCCCCTGCCCCGGGCAGCCCCAATGGGGAGTGTTGCTCAGTGAGT
  ACTGCAGGGGGCTCCCCGGAGGAGGAGCTGCCCCTGCCAGCCTTCGACAAGTTGAGTCCCTACCCTACCC
  CGTCTCCACCACACCCATTATACCCTGGTAGGAAGGTGATAGAATTCTCTGAGGACAAGATCCGAATTCC
  TCGGAACAGCCCCCTCCCCAACTGTACCTATGCCACCCGGCAGGCCATCTCCTTGAGCCTGGTGGAGGAC
  GGCAGTGAGCGGGCCCACCGCAGCTCAGTGCCTAGCAGTCCTGCCTCTGCCCAAGGGTCGCCCCATCACC
  AGCCTAGTCCAGCTCCCTCAGCACTGAGTGCCCCAGCTAGTTCGGCCAGCTCGGAGGAGGACCTGCTGGC
  CAGCTGGCAGCGTGCATTTGTGGACCGCACCCCGCCCCCTGCTGCCGTGGTCCAGCGCACGGCCTTTGGA
  CGAGACTCACTGCCCGAGCTACAATTGCATTTCTCCCCGGGCCACTCCACAGCCCCACCGCCTTCCCCGC
  ACCGTGAGCGGGGACTTGTGCTGCCAGCAGAACCTGACTCTGGCTTTCCTCAAGACGAGGAAGAGGAAAT
  GCTGAACCTGCCTGTCAGCCCCGAGGAAGAGCGTCAGAGCCTGCTGCCTGATAAAGAGGGCACCGAGGAG
  GCCTCTGGCCCTTCTCATGTTGATGGCAGGGCCTGGCCTCTCCCCAGTCCCAGCCGCCCCCAGCGCAGCC
  CCAAGAGAATGGGAGTACACCATCTCCACCGCAAGGACAGCCTGACGCAGGCACAGGAGCAGGGCACTGT
  GCTCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230438 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSAAPAKKPYRKAPPEHRELRLEIPVSRLEQEESLTDAERMKLLQQENEELRKRLASATRRTEALEREL
  EIGQDCLELELGQSREELDKFKDKFRRLQNSYTASQRTNQELEDKLHALIKKAEMDRKTLDWEIVELTNK
  LLDARNTINKLEELNERYRLDCNLAVQLLKCNKSHFRNHKLADLPCELQDMVRKHLRSGQEVASPSPSPS
  SSLSPGAVVPTSVIARVLEKPESLLLNSAQSGSAGRPLAEDVFVHVDMSGGDPASPPAPGSPNGECCSVS
  TAGGSPEEELPLPAFDKLSPYPTPSPPHPLYPGRKVIEFSEDKIRIPRNSPLPNCTYATRQAISLSLVED
  GSERAHRSSVPSSPASAQGSPHHQPSPAPSALSAPASSASSEEDLLASWQRAFVDRTPPPAAVVQRTAFG
  RDSLPELQLHFSPGHSTAPPPSPHRERGLVLPAEPDSGFPQDEEEEMLNLPVSPEEERQSLLPDKEGTEE
  ASGPSHVDGRAWPLPSPSRPQRSPKRMGVHHLHRKDSLTQAQEQGTVLS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252474
• ORF Size: 1617 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252474.1, NP_001239403.1
• RefSeq Size: 2462 bp
• RefSeq ORF: 1620 bp
• Locus ID: 74094
• UniProt ID: Q9DCD5
• Cytogenetics: 17 C
• MW: 59.4 kDa
• Gene Summary: Plays a role in regulating the structure of the Golgi apparatus.[UniProtKB/Swiss-Prot Function]