Cttn (NM_001252572) Mouse Tagged ORF Clone

CAT#: MR230309

Cttn (myc-DDK-tagged) - Mouse cortactin (Cttn), transcript variant 2

Product Images

Circular map for MR230309

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Cttn
• Synonyms: 1110020L01Rik; Ems1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230309 representing NM_001252572
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAAAGCCTCTGCAGGCCATGCTGTGTCCATCACGCAGGATGATGGAGGAGCTGATGACTGGGAGA
  CTGATCCTGATTTTGTGAATGATGTGAGTGAAAAGGAGCAGAGATGGGGTGCTAAAACCGTGCAGGGATC
  GGGGCACCAGGAACACATCAACATTCACAAGCTTCGAGAGAATGTCTTCCAAGAACACCAGACGCTCAAG
  GAGAAGGAGCTGGAAACGGGACCCAAGGCTTCCCACGGCTATGGCGGGAAGTTCGGTGTGGAGCAGGATA
  GGATGGACAGATCAGCCGTGGGCCATGAGTACCAGTCGAAGCTTTCCAAGCACTGCTCACAAGTGGACTC
  GGTCCGGGGCTTCGGAGGCAAGTTCGGTGTCCAGATGGACAGGGTGGATCAGTCTGCTGTAGGCTTTGAA
  TACCAGGGGAAGACTGAGAAGCATGCCTCCCAGAAAGACTACTCTAGTGGCTTCGGTGGCAAATACGGTG
  TGCAAGCTGACCGTGTAGACAAGAGTGCCGTGGGCTTTGACTACCAGGGCAAGACGGAGAAGCATGAGTC
  TCAGAAAGATTACTCCAAAGGTTTTGGTGGCAAATATGGGATTGACAAGGACAAGGTGGATAAAAGTGCT
  GTGGGCTTTGAGTATCAAGGCAAGACAGAGAAGCACGAATCCCAGAAAGACTATGTAAAAGGCTTTGGAG
  GAAAGTTTGGTGTGCAGACAGACAGACAGGACAAGTGTGCCCTTGGCTGGGACCATCAGGAGAAGCTGCA
  GCTGCATGAATCCCAAAAAGACTATGCCAAAGGATTCGGCGGGAAGTATGGGGTGCAGAAGGATCGGATG
  GACAAGAATGCATCCACCTTTGAAGAAGTGGTCCAGGTGCCATCTGCCTATCAGAAGACTGTCCCCATTG
  AGGCCGTAACCAGCAAAACCAGTAATATCCGTGCTAACTTTGAAAACCTGGCAAAGGAGAGAGAGCAGGA
  GGACAGGCGGAAGGCAGAAGCCGAGAGAGCTCAGCGGATGGCCAAAGAAAGACAGGAGCAGGAGGAGGCG
  CGCAGGAAGCTGGAAGAGCAAGCCAGAGCCAAGAAGCAGACGCCCCCTGCATCCCCTAGTCCTCAACCAA
  TTGAAGACAGACCACCCTCCAGCCCCATCTATGAGGATGCAGCTCCGTTCAAGGCCGAGCCGAGCTACCG
  AGGTAGCGAACCTGAGCCTGAGTACAGCATCGAGGCCGCAGGCATTCCTGAGGCTGGCAGCCAGCAAGGC
  CTGACCTATACATCAGAGCCCGTGTACGAGACTACAGAGGCTCCTGGCCACTATCAAGCAGAGGATGACA
  CCTACGATGGGTATGAGAGTGACCTGGGCATCACAGCCATCGCCCTGTATGACTACCAGGCTGCTGGCGA
  TGATGAGATCTCCTTTGACCCTGATGACATCATCACCAACATAGAAATGATTGACGATGGCTGGTGGCGT
  GGGGTGTGCAAGGGCAGATACGGGCTCTTCCCAGCCAACTATGTGGAGCTGCGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230309 representing NM_001252572
  Red=Cloning site Green=Tags(s)
  
  MWKASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLK
  EKELETGPKASHGYGGKFGVEQDRMDRSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFE
  YQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQKDYSKGFGGKYGIDKDKVDKSA
  VGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYAKGFGGKYGVQKDRM
  DKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFENLAKEREQEDRRKAEAERAQRMAKERQEQEEA
  RRKLEEQARAKKQTPPASPSPQPIEDRPPSSPIYEDAAPFKAEPSYRGSEPEPEYSIEAAGIPEAGSQQG
  LTYTSEPVYETTEAPGHYQAEDDTYDGYESDLGITAIALYDYQAAGDDEISFDPDDIITNIEMIDDGWWR
  GVCKGRYGLFPANYVELRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001252572
• ORF Size: 1527 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001252572.1, NP_001239501.1
• RefSeq Size: 3112 bp
• RefSeq ORF: 1530 bp
• Locus ID: 13043
• Cytogenetics: 7 F5
• MW: 57.5 kDa
• Gene Summary: Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:17403031). Plays a role in the formation of lamellipodia and in cell migration (By similarity). Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (PubMed:22262902). Plays a role in the invasiveness of cancer cells, and the formation of metastases (By similarity). Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (By similarity). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane (By similarity).[UniProtKB/Swiss-Prot Function]