Rbpj (NM_001277116) Mouse Tagged ORF Clone

CAT#: MR230298

Rbpj (myc-DDK-tagged) - Mouse recombination signal binding protein for immunoglobulin kappa J region (Rbpj), transcript variant 4

Product Images

Circular map for MR230298

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rbpj
• Synonyms: AI843960; CBF1; Igkjrb; Igkrsbp; RBP-J; RBP-J kappa; RBP-Jkappa; RBPjk; Rbpsuh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR230298 representing NM_001277116
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGTTGCTGGGATTTGAACTCCTGACCTTCAGAAGAGCAGTCGGGTGCTCTTACCCATTGAGCCATC
  TCACCAGCCCGAAGTTTGGTGAGCGGCCTCCACCCAAACGACTCACTAGGGAAGCTATGCGAAATTATTT
  AAAAGAACGAGGGGATCAAACAGTGCTCATTCTTCATGCAAAAGTTGCACAGAAGTCTTACGGAAATGAA
  AAACGATTTTTTTGCCCTCCTCCTTGTGTGTATCTTATGGGCAGTGGTTGGAAGAAAAAAAAAGAGCAAA
  TGGAACGAGATGGATGTTCTGAACAAGAGTCTCAACCCTGTGCGTTTATTGGAATAGGAAACAGTGACCA
  AGAAATGCAGCAGCTGAACTTGGAAGGGAAGAACTACTGTACAGCCAAAACATTGTACATATCTGATTCA
  GACAAGAGAAAACATTTCATGTTGTCTGTAAAGATGTTCTATGGCAACAGCGATGACATTGGTGTGTTCC
  TCAGCAAGCGGATAAAGGTCATCTCCAAACCCTCCAAAAAGAAGCAGTCACTGAAGAATGCTGACTTGTG
  CATTGCTTCAGGAACGAAGGTGGCACTGTTCAATCGCCTTCGGTCCCAGACAGTTAGTACCAGGTACCTG
  CATGTAGAAGGAGGGAATTTCCACGCCAGTTCACAACAGTGGGGAGCATTTTACATCCATCTCTTGGACG
  ACGACGAGTCGGAAGGAGAGGAGTTCACAGTTAGAGATGGCTACATCCATTACGGGCAGACTGTCAAGCT
  TGTGTGCTCAGTGACTGGCATGGCACTCCCAAGATTGATAATTAGGAAAGTTGATAAGCAGACGGCATTA
  CTGGATGCAGACGACCCTGTATCACAACTCCACAAATGTGCATTTTACCTTAAGGATACAGAAAGAATGT
  ACTTGTGCCTTTCTCAAGAAAGAATAATCCAATTTCAGGCCACTCCATGTCCAAAAGAACAAAATAAGGA
  AATGATAAACGATGGAGCTTCCTGGACAATCATTAGCACAGACAAGGCCGAGTACACGTTCTATGAGGGA
  ATGGGCCCCGTCCTTGCCCCAGTCACCCCTGTGCCTGTCGTAGAAAGTCTTCAGTTGAATGGCGGCGGGG
  ACGTAGCAATGCTTGAACTTACAGGACAAAACTTTACTCCAAATTTAAGAGTGTGGTTTGGGGATGTAGA
  AGCCGAAACAATGTACAGATGTGGAGAGAGCATGCTCTGTGTGGTCCCAGACATTTCTGCATTCCGGGAA
  GGTTGGAGATGGGTCCGCCAGCCAGTCCAGGTTCCAGTGACTTTGGTCCGAAATGACGGGGTCATTTACT
  CCACCAGCCTTACCTTCACCTACACACCAGAGCCAGGGCCGAGGCCACACTGCAGCGCTGCAGGAGCGAT
  TCTCAGAGCCAACTCCAGCCAAGTGCCCTCCAATGAGTCAAACACAAACAGCGAGGGGAATTACACAAAT
  GCCAGCACAAATTCTACCAGTGTCACATCGTCCACAGCAACCGTGGTGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR230298 representing NM_001277116
  Red=Cloning site Green=Tags(s)
  
  MWLLGFELLTFRRAVGCSYPLSHLTSPKFGERPPPKRLTREAMRNYLKERGDQTVLILHAKVAQKSYGNE
  KRFFCPPPCVYLMGSGWKKKKEQMERDGCSEQESQPCAFIGIGNSDQEMQQLNLEGKNYCTAKTLYISDS
  DKRKHFMLSVKMFYGNSDDIGVFLSKRIKVISKPSKKKQSLKNADLCIASGTKVALFNRLRSQTVSTRYL
  HVEGGNFHASSQQWGAFYIHLLDDDESEGEEFTVRDGYIHYGQTVKLVCSVTGMALPRLIIRKVDKQTAL
  LDADDPVSQLHKCAFYLKDTERMYLCLSQERIIQFQATPCPKEQNKEMINDGASWTIISTDKAEYTFYEG
  MGPVLAPVTPVPVVESLQLNGGGDVAMLELTGQNFTPNLRVWFGDVEAETMYRCGESMLCVVPDISAFRE
  GWRWVRQPVQVPVTLVRNDGVIYSTSLTFTYTPEPGPRPHCSAAGAILRANSSQVPSNESNTNSEGNYTN
  ASTNSTSVTSSTATVVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001277116
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001277116.1, NP_001264045.1
• RefSeq Size: 5590 bp
• RefSeq ORF: 1524 bp
• Locus ID: 19664
• Cytogenetics: 5 29.37 cM
• MW: 57.2 kDa
• Gene Summary: Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations (PubMed:7566092). Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen) (By similarity). Negatively regulates the phagocyte oxidative burst in response to bacterial infection by repressing transcription of NADPH oxidase subunits (PubMed:26194095).[UniProtKB/Swiss-Prot Function]