Syt6 (NM_001276679) Mouse Tagged ORF Clone

CAT#: MR229843

Syt6 (myc-DDK-tagged) - Mouse synaptotagmin VI (Syt6), transcript variant 4

Product Images

Circular map for MR229843

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Syt6
• Synonyms: 3110037A08Rik; AW048930; sytVI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229843 representing NM_001276679
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTGGAGGAAGAAAGAGGCCTCCAGCCCTTCTTCTGCTAACCCTGCTTCAGAGACTCTCCAGAGCC
  CCAGCTCCAGAGGCAACATGGCGGATAAGCTGAAGGACCCCAGTGCCCTGGGCTTCCTGGAGGCAGCTGT
  GAAGATCAGCCACACTTCCCCAGACATCCCAGCCGAGGTGCAGATGTCGGTCAAAGAGCACATCATGCGC
  CACACAAAGCTGCAGCGACAGACCACAGAGCCAGCATCCTCCACCAGGCACACATCCTTCAAGCGCCACC
  TGCCACGACAGATGCATGTCTCCAGCGTGGACTATGGCAATGAGCTGCCGCCGGCTGCCGCCGAGCAGCC
  CACCAGTATTGGCCGTATCAAGCCTGAGCTTTACAAGCAGAAGTCAGTGGATGGGGATGATGCCAAGTCG
  GAGGCCGCCAAGAGCTGTGGGAAGATCAACTTCAGCCTCCGCTATGACTATGAAAGCGAGACGCTGATTG
  TGCGCATCCTGAAGGCCTTTGACCTCCCTGCCAAGGACTTTTGCGGAAGTTCTGACCCTTATGTTAAGAT
  CTACCTCCTGCCTGACCGCAAGTGCAAGCTGCAGACCCGGGTGCACCGCAAGACCCTGAACCCCACCTTT
  GATGAGAACTTCCACTTCCCCGTGCCCTACGAGGAGCTGGCTGACCGCAAGCTGCATCTCAGTGTCTTTG
  ACTTTGACCGCTTCTCCCGCCATGACATGATTGGGGAGGTCATCCTGGACAATCTCTTTGAGGCCTCTGA
  CCTGTCCCGGGAGACCTCCATCTGGAAGGACATCCAGTACGCTACTAGTGAAAGTGTGGACTTGGGAGAG
  ATCATGTTCTCCCTCTGCTACCTGCCCACAGCAGGCAGGCTCACCCTCACAGTGATCAAGTGTCGAAATC
  TCAAAGCAATGGACATCACAGGCTACTCAGATCCCTACGTCAAAGTATCCCTACTGTGTGATGGACGGAG
  GCTGAAAAAGAAGAAAACGACCATAAAAAAGAACACCCTAAACCCCATCTACAACGAGGCCATCATCTTT
  GACATCCCCCCAGAAAACATGGACCAAGTGAGCCTGCTCATCTCCGTTATGGACTATGATAGAGTTGGCC
  ACAACGAGATCATAGGAGTCTGTAGAGTGGGGATCAATGCTGAAGGCCTTGGCAGGGACCACTGGAATGA
  GATGTTGGCGTACCCACGAAAGCCCATTGCGCACTGGCACTCCTTGGTGGAGGTAAAGAAATCCTTCAAA
  GAGGGGACCCCTCGGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229843 representing NM_001276679
  Red=Cloning site Green=Tags(s)
  
  MPWRKKEASSPSSANPASETLQSPSSRGNMADKLKDPSALGFLEAAVKISHTSPDIPAEVQMSVKEHIMR
  HTKLQRQTTEPASSTRHTSFKRHLPRQMHVSSVDYGNELPPAAAEQPTSIGRIKPELYKQKSVDGDDAKS
  EAAKSCGKINFSLRYDYESETLIVRILKAFDLPAKDFCGSSDPYVKIYLLPDRKCKLQTRVHRKTLNPTF
  DENFHFPVPYEELADRKLHLSVFDFDRFSRHDMIGEVILDNLFEASDLSRETSIWKDIQYATSESVDLGE
  IMFSLCYLPTAGRLTLTVIKCRNLKAMDITGYSDPYVKVSLLCDGRRLKKKKTTIKKNTLNPIYNEAIIF
  DIPPENMDQVSLLISVMDYDRVGHNEIIGVCRVGINAEGLGRDHWNEMLAYPRKPIAHWHSLVEVKKSFK
  EGTPRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276679
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276679.1, NP_001263608.1
• RefSeq Size: 1599 bp
• RefSeq ORF: 1281 bp
• Locus ID: 54524
• Cytogenetics: 3 F2.2
• MW: 48.8 kDa
• Gene Summary: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (By similarity). May mediate Ca(2+)-regulation of exocytosis in acrosomal reaction in sperm (PubMed:15774481).[UniProtKB/Swiss-Prot Function]