Osgepl1 (NM_001285839) Mouse Tagged ORF Clone

CAT#: MR229781

Osgepl1 (myc-DDK-tagged) - Mouse O-sialoglycoprotein endopeptidase-like 1 (Osgepl1), transcript variant 1

Product Images

Circular map for MR229781

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Osgepl1
• Synonyms: 2610001M19Rik; AA416452
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229781 representing NM_001285839
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAATGTTAAGAAGGACAGCAGGAGCTATTCCCAAGCCCCCAAAGAGTAAAGTTTATGGATTTTTAA
  GAAGATTTAGTGTTCATCCCAGAACTCTCTCTTGTCATAAACTGGTCCTGGGAATTGAAACCAGCTGTGA
  TGACACAGGAGCGGCTGTGGTGGATGAAACTGGGAATGTGCTGGGGGAAGCACTGCACTCCCAAACTCAG
  GTTCATCTGAAAACAGGTGGGATTGTTCCTCCAGTAGCTCAACAACTTCACAGAGAAAATATTCAACGAA
  TAGTAGAAGAAACTCTTTCTGCCTGTAGAATCACCCCAAGCGATCTCTCAGCAATTGCAACTACCATCAA
  ACCGGGACTGGCCCTAAGCTTGGGAGTTGGCTTATCCTTTAGCTTACAGCTAGTAAATCAGTTTAAAAAG
  CCATTCATCCCGATTCATCACATGGAGGCTCACGCACTGACTATTAGGCTCACCAATAAAGTAGAATTTC
  CTTTTTTAGTTCTTTTGATTTCTGGCGGTCACTGCCTGTTGGCATTAGTCCAAGGTGTTTCCGATTTCCT
  GCTCCTTGGGAAGTCTTTGGACATAGCACCAGGCGACATGCTTGACAAGGTGGCAAGAAGACTTTCTTTA
  ATCAAACATCCAGAATGTTCTACAATGAGTGGTGGAAAAGCTATAGAACAGTTGGCCAAAGACGGAAATA
  GATTCCATTTTACTATCAATCCACCTATGCAGAATGCTAAGAATTGCGATTTTTCTTTCACGGGACTTCA
  ACATATTACTGATAAGCTAATAACACACAAGGAAAAAGAAGAAGGCATTGAGAAGGGGCAAATCCTGTCA
  TCAGCTGCAGACATTGCTGCTGCGGTACAGCATGCAACAGCGTGCCACCTTGCGAAAAGAACACATCGCG
  CTATTCTGTTTTGCAAGCAGAAAAATTTGCTCTCTCCAGCTAACGCAGTATTAGTTGTATCTGGAGGTGT
  TGCAAGTAACTTGTACATCCGAAAAGCATTGGAAATTGTCGCAAATGCAACGCAGTGCACGTTGTTGTGT
  CCACCTCCAAGACTGTGCACTGACAATGGCATCATGATTGCATGGAATGGAATTGAAAGATTACGTGCTG
  GCTTGGGCGTTTTACATGATGTAGAAGACATCCGATATGAACCAAAATGTCCTCTTGGAGTAGACATATC
  CAGAGAAGTTGCAGAAGCTGCCATAAAAGTACCGCGATTAAAAATGGCACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229781 representing NM_001285839
  Red=Cloning site Green=Tags(s)
  
  MLMLRRTAGAIPKPPKSKVYGFLRRFSVHPRTLSCHKLVLGIETSCDDTGAAVVDETGNVLGEALHSQTQ
  VHLKTGGIVPPVAQQLHRENIQRIVEETLSACRITPSDLSAIATTIKPGLALSLGVGLSFSLQLVNQFKK
  PFIPIHHMEAHALTIRLTNKVEFPFLVLLISGGHCLLALVQGVSDFLLLGKSLDIAPGDMLDKVARRLSL
  IKHPECSTMSGGKAIEQLAKDGNRFHFTINPPMQNAKNCDFSFTGLQHITDKLITHKEKEEGIEKGQILS
  SAADIAAAVQHATACHLAKRTHRAILFCKQKNLLSPANAVLVVSGGVASNLYIRKALEIVANATQCTLLC
  PPPRLCTDNGIMIAWNGIERLRAGLGVLHDVEDIRYEPKCPLGVDISREVAEAAIKVPRLKMAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001285839
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285839.1, NP_001272768.1
• RefSeq Size: 1868 bp
• RefSeq ORF: 1245 bp
• Locus ID: 72085
• UniProt ID: Q6PEB4
• Cytogenetics: 1 26.99 cM
• MW: 44.9 kDa
• Gene Summary: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance.[UniProtKB/Swiss-Prot Function]