Vsx2 (NM_001301427) Mouse Tagged ORF Clone

CAT#: MR229606

Vsx2 (myc-DDK-tagged) - Mouse visual system homeobox 2 (Vsx2), transcript variant 1

Product Images

Circular map for MR229606

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vsx2
• Synonyms: Chx10; Hox-1; Hox-10; Hox1; Hox10; or
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229606 representing NM_001301427
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGGGAAAGCGGGGGAAGCGCTGAGCAAGCCCAAATCCGAGACAGTGGCCAAGAGTACCTCGGGGG
  GCGCCCCGGCCAGGTGCACAGGGTTCGGCATCCAGGAGATCCTAGGCTTGAACAAGGAGCCTCCCAGCTC
  CCACCCGCGGGCTGCCCTCGACGGCCTGGCCCCTGGGCATTTGTTAGCTGCGCGCTCCGTTCTCAGCCCG
  GCAGGAGTGGGCAGCATGGGGCTGCTGGGGCCCGGGGGACTTCCCGGCTTCTACACACAGCCCACCTTCT
  TGGAAGTGCTGTCCGATCCGCAGAGCGTCCACTTGCAGCCACTAGGCAGAGCGTCGGGGCCGCTGGACAC
  CAGCCAGACGGCCAGCTCCGATTCCGAAGATGTTTCCTCCAGTGACCGAAAAATGTCCAAATCGGCTTTA
  AACCAGACCAAGAAGCGTAAGAAGCGGCGACACAGGACAATCTTTACTTCCTACCAGCTAGAGGAGCTGG
  AGAAAGCATTCAATGAAGCCCACTACCCAGATGTCTACGCCCGGGAGATGCTGGCCATGAAAACGGAGCT
  CCCAGAAGACAGGATACAGGTGTGGTTCCAGAACCGCAGAGCCAAGTGGAGGAAGAGGGAGAAGTGCTGG
  GGCCGGAGCAGCGTCATGGCTGAGTATGGTCTCTATGGAGCCATGGTGAGGCACTCGATCCCCCTGCCAG
  AGTCTATCCTCAAGTCTGCCAAGGATGGCATAATGGATTCCTGTGCCCCATGGCTACTGGTTCAAGATGG
  CTTTCCCAGGCGCTTTTCTAAACCCGAATACCAACAATTCTTTTTAGGGATGCACAAAAAGTCGCTGGAG
  GCAGCAGCTGAGTCGGGAAGGAAGCCCGAGGTGGAACGCCAGGCCCTGCCCAAGCTCGACAAGATGGAGC
  AGGAGGAACGGGCCCCAGAGGCCCAGGCAGCCATCTCCCAGGAAGAACTGAGGGAGAACAGCATTGCCGC
  CCTCCGAGCCAAAGCTCAGGAACACAGCACCAAGGTTCTGGGGACTGTATCTGGGCCTGACAGCCTGGCC
  AGAAATGCTGAGAAGCCAGAAGAAGAGGACGCCACGGAGGAAGACAGGCCAGCTGAGAAGCTCAGCCCAC
  CGCAGCTGGAGGACATGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229606 representing NM_001301427
  Red=Cloning site Green=Tags(s)
  
  MTGKAGEALSKPKSETVAKSTSGGAPARCTGFGIQEILGLNKEPPSSHPRAALDGLAPGHLLAARSVLSP
  AGVGSMGLLGPGGLPGFYTQPTFLEVLSDPQSVHLQPLGRASGPLDTSQTASSDSEDVSSSDRKMSKSAL
  NQTKKRKKRRHRTIFTSYQLEELEKAFNEAHYPDVYAREMLAMKTELPEDRIQVWFQNRRAKWRKREKCW
  GRSSVMAEYGLYGAMVRHSIPLPESILKSAKDGIMDSCAPWLLVQDGFPRRFSKPEYQQFFLGMHKKSLE
  AAAESGRKPEVERQALPKLDKMEQEERAPEAQAAISQEELRENSIAALRAKAQEHSTKVLGTVSGPDSLA
  RNAEKPEEEDATEEDRPAEKLSPPQLEDMA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001301427
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001301427.1, NP_001288356.1
• RefSeq Size: 3276 bp
• RefSeq ORF: 1143 bp
• Locus ID: 12677
• Cytogenetics: 12 39.28 cM
• MW: 42.3 kDa
• Gene Summary: This gene encodes a member of the Vsx (visual system homeobox) family which belongs to the larger PRD homeobox class. The encoded protein is required for eye organogenesis and controls retinal development. Disruption of this gene is associated with ocular retardation J (orJ), a mouse disease which causes microphthalmia, retinal degeneration and optic nerve aplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]