Fez2 (NM_001285949) Mouse Tagged ORF Clone

CAT#: MR229390

Fez2 (myc-DDK-tagged) - Mouse fasciculation and elongation protein zeta 2 (zygin II) (Fez2), transcript variant 4

Product Images

Circular map for MR229390

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Fez2
• Synonyms: 9030616F16; D17Ertd315e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR229390 representing NM_001285949
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGACGGGGACTGGCAGGATTTCTATGAGTTCCAGGAGCCGGCCGGGAGTGTCCAGGAACAGG
  AGAACTGTAACGCGAGCCCGGAGGCTGGCGCGGGGGCACACGCGGGCGGCGACAGCTTCCCGGCGCTGGC
  TTCCAGCCTGGAGGAGAAGCTGAGCCTGTGCTTCCGCCCCACGTCGGAGGCCGAGCCCCCACGGGCGGCC
  GTGCGGCCCATCACGGAGTGCAGCCTCCTGCAAGGGGACGAGATTTGGAATGCCCTGACAGATAACTATG
  GGAATGTCATGCCTGTAGACTGGAAGTCGTCCCATACAAGGACCTTGCATTTGCTTACTCTGAACCTCTC
  GGAGAAAGGGATGAACGACGGCTTGCTCTTTGACGCCTCGGATGAGGAGGAGCTGAGGGAGCAGTTGGAC
  ATGCATTCGATCATTGTCTCCTGTGTCAACGAAGAGCCTCTCTTCACTGCAGACCAGGTGATTGAAGAAA
  TTGAAGAAATGATGCAGGAGTCACCAGACCCAGAAGACGACGAGACCCCCACACAGTCAGACCGGCTCTC
  TATGCTCTCCCAGGAGATTCAGACTCTCAAGAGGTCTAGCATGAGCAGCTATGAAGAGCGAGTGAAAAGG
  CTGTCGGTGTCTGAGCTAAACGAACTGCTGGAAGAGATCGAGGCAGCCATTAAGCAGTACTCTGAGGAGC
  TGGTGCAGCAGCTGGCCCTGCGAGATGAACTCGAGTTCGAGAAGGAAGTGGAAAACAGCTTCATTTCCGC
  TCTGATTGAGGTGCAGAACAAGCAGAAAGAACACAAAGAGACAGCCAAGAAGAAGAAGAAGCTCAAAAGT
  GGCAGCTCTCAGAACGGGAGGAGTGAGAGAAGTCACATGCCGGGCACATATCTGACGACAGTCATCCCTT
  ACGAGAAGAAGAGTGGCCCGCCGTCCGTGGAAGATCTTCAGATACTAACCAAAATCCTGCATGCCATGAA
  GGAGGACAGTGAGAAAGTTCCAAGCTTGTTAACCGACTATATACTGAAAGGCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR229390 representing NM_001285949
  Red=Cloning site Green=Tags(s)
  
  MAADGDWQDFYEFQEPAGSVQEQENCNASPEAGAGAHAGGDSFPALASSLEEKLSLCFRPTSEAEPPRAA
  VRPITECSLLQGDEIWNALTDNYGNVMPVDWKSSHTRTLHLLTLNLSEKGMNDGLLFDASDEEELREQLD
  MHSIIVSCVNEEPLFTADQVIEEIEEMMQESPDPEDDETPTQSDRLSMLSQEIQTLKRSSMSSYEERVKR
  LSVSELNELLEEIEAAIKQYSEELVQQLALRDELEFEKEVENSFISALIEVQNKQKEHKETAKKKKKLKS
  GSSQNGRSERSHMPGTYLTTVIPYEKKSGPPSVEDLQILTKILHAMKEDSEKVPSLLTDYILKGW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001285949
• ORF Size: 1035 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001285949.1, NP_001272878.1
• RefSeq Size: 3767 bp
• RefSeq ORF: 1038 bp
• Locus ID: 225020
• Cytogenetics: 17 48.57 cM
• MW: 39.3 kDa
• Gene Summary: Involved in axonal outgrowth and fasciculation.[UniProtKB/Swiss-Prot Function]